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In vitro and in vivo survival and colonic adhesion of Pediococcus acidilactici MTCC5101 in human gut.

Balgir PP, Kaur B, Kaur T, Daroch N, Kaur G - Biomed Res Int (2013)

Bottom Line: Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers.The effect on wellness was assessed by studying blood biochemical parameters of the volunteers.The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Punjabi University, Punjab, Patiala 147 002, India.

ABSTRACT
The present study aims to investigate the probiotic nature of Pediococcus acidilactici MTCC5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples. The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.

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Related in: MedlinePlus

2% agarose gel showing 323 bp DNA fragment amplified using plasmids from fecal samples (a) M: marker pUC 19/Msp Digest, C(+): positive control, Lane 1–10: 5th day samples, C(−): negative control. (b) M: marker pUC 19/Msp digest, C(+): positive control, lane 1–10: 10th day's samples, C(−): Negative control. (c) M: Marker pUC 19/Msp Digest, C(+): Positive control, Lane 1–10: 20th day's samples, C(−): negative control. (d) M: marker pUC 19/Msp Digest, C(+): positive control, lane 1–10: 30th day's samples, C(−): negative control.
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fig2: 2% agarose gel showing 323 bp DNA fragment amplified using plasmids from fecal samples (a) M: marker pUC 19/Msp Digest, C(+): positive control, Lane 1–10: 5th day samples, C(−): negative control. (b) M: marker pUC 19/Msp digest, C(+): positive control, lane 1–10: 10th day's samples, C(−): Negative control. (c) M: Marker pUC 19/Msp Digest, C(+): Positive control, Lane 1–10: 20th day's samples, C(−): negative control. (d) M: marker pUC 19/Msp Digest, C(+): positive control, lane 1–10: 30th day's samples, C(−): negative control.

Mentions: Results of the PCR analysis coincide with count data that indicates detectable levels of P. acidilactici MTCC5101 in all the volunteers after a 4-week intervention. Figures 2(a) and 2(b) depict agarose gel analysis of 323 bp fragment of partial pedA gene amplified from isolated whole LAB plasmids of 5th and 10th day's fecal samples. Intensity of the PCR amplicon is lesser in the 5th day's samples which show that the establishment of the probiotic strain requires a little longer time to persist and colonize in the human gut. The fragment of the expected size was obtained in nearly all 10th day's samples and the bands in agarose gels were more prominent as compared to 5th day which confirms that the pediococci have started gut colonization. A similar pattern is observed in the 20th and 30th day's fecal samples on 2% agarose (Figures 2(c) and 2(d)). The band intensity was more prominent as compared to 5th and 10th day samples which indicate that the strain has colonized in the GI tract of volunteers, hence proving that colonization progresses in a time-dependent manner.


In vitro and in vivo survival and colonic adhesion of Pediococcus acidilactici MTCC5101 in human gut.

Balgir PP, Kaur B, Kaur T, Daroch N, Kaur G - Biomed Res Int (2013)

2% agarose gel showing 323 bp DNA fragment amplified using plasmids from fecal samples (a) M: marker pUC 19/Msp Digest, C(+): positive control, Lane 1–10: 5th day samples, C(−): negative control. (b) M: marker pUC 19/Msp digest, C(+): positive control, lane 1–10: 10th day's samples, C(−): Negative control. (c) M: Marker pUC 19/Msp Digest, C(+): Positive control, Lane 1–10: 20th day's samples, C(−): negative control. (d) M: marker pUC 19/Msp Digest, C(+): positive control, lane 1–10: 30th day's samples, C(−): negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794518&req=5

fig2: 2% agarose gel showing 323 bp DNA fragment amplified using plasmids from fecal samples (a) M: marker pUC 19/Msp Digest, C(+): positive control, Lane 1–10: 5th day samples, C(−): negative control. (b) M: marker pUC 19/Msp digest, C(+): positive control, lane 1–10: 10th day's samples, C(−): Negative control. (c) M: Marker pUC 19/Msp Digest, C(+): Positive control, Lane 1–10: 20th day's samples, C(−): negative control. (d) M: marker pUC 19/Msp Digest, C(+): positive control, lane 1–10: 30th day's samples, C(−): negative control.
Mentions: Results of the PCR analysis coincide with count data that indicates detectable levels of P. acidilactici MTCC5101 in all the volunteers after a 4-week intervention. Figures 2(a) and 2(b) depict agarose gel analysis of 323 bp fragment of partial pedA gene amplified from isolated whole LAB plasmids of 5th and 10th day's fecal samples. Intensity of the PCR amplicon is lesser in the 5th day's samples which show that the establishment of the probiotic strain requires a little longer time to persist and colonize in the human gut. The fragment of the expected size was obtained in nearly all 10th day's samples and the bands in agarose gels were more prominent as compared to 5th day which confirms that the pediococci have started gut colonization. A similar pattern is observed in the 20th and 30th day's fecal samples on 2% agarose (Figures 2(c) and 2(d)). The band intensity was more prominent as compared to 5th and 10th day samples which indicate that the strain has colonized in the GI tract of volunteers, hence proving that colonization progresses in a time-dependent manner.

Bottom Line: Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers.The effect on wellness was assessed by studying blood biochemical parameters of the volunteers.The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Punjabi University, Punjab, Patiala 147 002, India.

ABSTRACT
The present study aims to investigate the probiotic nature of Pediococcus acidilactici MTCC5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples. The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.

Show MeSH
Related in: MedlinePlus