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Interleukin-1β secretion in hippocampal sclerosis patients with mesial temporal lobe epilepsy.

Dundar NO, Aktekin B, Ekinci NC, Sahinturk D, Yavuzer U, Yegin O, Haspolat S - Neurol Int (2013)

Bottom Line: However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated.Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05).Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Neurology, Izmir Katip Celebi University , Izmir.

ABSTRACT
Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is a common medically intractable epilepsy syndrome. Although pathogenesis of HS still remains highly controversial, genetics may play a role as a predisposing factor. Previous evidence in a Japanese population revealed that the homozygotes for allele T at position -511 of the interleukin (IL)-1β gene promoter region (IL-1β-511 T/T) confers susceptibility to the development of HS. However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated. This study aimed to analyze the distribution of this particular polymorphism in a group of Turkish HS patients and correlate the polymorphism with IL-1β secretion from the lymphocytes, thus revealing a functional role for IL-1β in the etiopathogenesis of HS. A single base pair polymorphism at position -511 in the promoter region of the IL-1β gene was analyzed. The spontaneous and 1 ng/mL lipopolysaccharidestimulated production of IL-1β by peripheral blood mononuclear cells after 4 and 24 h of incubation were measured by ELISA method. The heterozygous type (-511 C/T) was the most common genotype. There was no difference in frequency of allele -511 T between patients and controls. Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05). Nevertheless, it was seen that patients who carry a T allele at position -511 of the IL-1β gene had increased IL-1β levels. T-allele carriage may be important. Only IL-1β secretion from the lymphocytes has been assessed in this study. Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.

No MeSH data available.


Related in: MedlinePlus

Spontaneous and stimulated levels of IL-1β in patients who carry and who do not carry T allele (pg/mL). Sti, stimulated; T+, patients who carry T allele; T-, patients who do not carry T allele.
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fig002: Spontaneous and stimulated levels of IL-1β in patients who carry and who do not carry T allele (pg/mL). Sti, stimulated; T+, patients who carry T allele; T-, patients who do not carry T allele.

Mentions: Levels of secreted IL-1β from the cultured lymphocytes incubated either with or without LPS were measured at three different time frames during the culture period (basal, 4 h and 24 h). However, no statistically significant difference was observed between the groups (Table 3). Since IL-1β-511 T allele has been suggested to be associated with increased in vitro production of IL-1β and that IL-1β is involved in HS pathogenesis,13,16 we also analyzed the IL-1β secretion between the patients and control individuals who carry the T allele at position -511 of the IL-1β gene. However, although spontaneous secretion levels of IL-1β were higher in MTLE-HS patients, there was no significant difference between the groups (Figure 1). In addition, despite the fact that both spontaneous and stimulated secretion levels of IL-1β were higher in MTLE-HS patients who carry the T allele with respect to those patients who do not, the difference was not statistically significant (Figure 2).


Interleukin-1β secretion in hippocampal sclerosis patients with mesial temporal lobe epilepsy.

Dundar NO, Aktekin B, Ekinci NC, Sahinturk D, Yavuzer U, Yegin O, Haspolat S - Neurol Int (2013)

Spontaneous and stimulated levels of IL-1β in patients who carry and who do not carry T allele (pg/mL). Sti, stimulated; T+, patients who carry T allele; T-, patients who do not carry T allele.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3794452&req=5

fig002: Spontaneous and stimulated levels of IL-1β in patients who carry and who do not carry T allele (pg/mL). Sti, stimulated; T+, patients who carry T allele; T-, patients who do not carry T allele.
Mentions: Levels of secreted IL-1β from the cultured lymphocytes incubated either with or without LPS were measured at three different time frames during the culture period (basal, 4 h and 24 h). However, no statistically significant difference was observed between the groups (Table 3). Since IL-1β-511 T allele has been suggested to be associated with increased in vitro production of IL-1β and that IL-1β is involved in HS pathogenesis,13,16 we also analyzed the IL-1β secretion between the patients and control individuals who carry the T allele at position -511 of the IL-1β gene. However, although spontaneous secretion levels of IL-1β were higher in MTLE-HS patients, there was no significant difference between the groups (Figure 1). In addition, despite the fact that both spontaneous and stimulated secretion levels of IL-1β were higher in MTLE-HS patients who carry the T allele with respect to those patients who do not, the difference was not statistically significant (Figure 2).

Bottom Line: However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated.Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05).Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Neurology, Izmir Katip Celebi University , Izmir.

ABSTRACT
Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is a common medically intractable epilepsy syndrome. Although pathogenesis of HS still remains highly controversial, genetics may play a role as a predisposing factor. Previous evidence in a Japanese population revealed that the homozygotes for allele T at position -511 of the interleukin (IL)-1β gene promoter region (IL-1β-511 T/T) confers susceptibility to the development of HS. However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated. This study aimed to analyze the distribution of this particular polymorphism in a group of Turkish HS patients and correlate the polymorphism with IL-1β secretion from the lymphocytes, thus revealing a functional role for IL-1β in the etiopathogenesis of HS. A single base pair polymorphism at position -511 in the promoter region of the IL-1β gene was analyzed. The spontaneous and 1 ng/mL lipopolysaccharidestimulated production of IL-1β by peripheral blood mononuclear cells after 4 and 24 h of incubation were measured by ELISA method. The heterozygous type (-511 C/T) was the most common genotype. There was no difference in frequency of allele -511 T between patients and controls. Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05). Nevertheless, it was seen that patients who carry a T allele at position -511 of the IL-1β gene had increased IL-1β levels. T-allele carriage may be important. Only IL-1β secretion from the lymphocytes has been assessed in this study. Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.

No MeSH data available.


Related in: MedlinePlus