Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.
Bottom Line: A specific interstitial deletion of chromosome 2 found in a high proportion of rAML is recognised as the initiating event.Although the deletion can be detected early following ionising radiation exposure by cytogenetic techniques, precise characterisation of the haematopoietic cells carrying the deletion and the study of their fate in vivo cannot be achieved.This study presents the first experimental evidence for the detection of this leukaemia initiating event in live leukemic cells.
Affiliation: Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, Oxfordshire, UK.Show MeSH
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Mentions: Both CBA/H Sfpi1GFP/GFP and CBA/H Sfpi1GFP/+ animals (generated from a homozygous male and wild type CBA/H female) were utilised in this study. All animals were bred and handled according to UK Home Office Animals (Scientific Procedures) Act 1986 and with guidance from the local ethical review committee on animal experiments. Details of the original construct, its localisation on mouse chromosome 2 and position of primers used for genotyping are presented in Fig. 1.
Affiliation: Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, Oxfordshire, UK.