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Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

Olme CH, Finnon R, Brown N, Kabacik S, Bouffler SD, Badie C - Leuk. Res. (2013)

Bottom Line: A specific interstitial deletion of chromosome 2 found in a high proportion of rAML is recognised as the initiating event.Although the deletion can be detected early following ionising radiation exposure by cytogenetic techniques, precise characterisation of the haematopoietic cells carrying the deletion and the study of their fate in vivo cannot be achieved.This study presents the first experimental evidence for the detection of this leukaemia initiating event in live leukemic cells.

View Article: PubMed Central - PubMed

Affiliation: Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, Oxfordshire, UK.

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Related in: MedlinePlus

Location of the GFP construct and a detailed view of the some of its features. The GFP construct is situated in the 3′ untranslated region of the Sfpi1 gene, located on mouse chromosome 2. The Sfpi1 gene is located in the AML minimal deleted region (mdr) between D2Mit126 and D2Mit185. Exons of the gene are denoted as boxes with the coding region in grey and introns as a black line, where exon 5 is specifically denoted. The direction of transcription is marked by arrows. Three primer binding sites forward A, reverse B and forward C are indicated. Stop: Stop codon, IRES: internal ribosome entry site, Neo: neomycin cassette.
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fig0010: Location of the GFP construct and a detailed view of the some of its features. The GFP construct is situated in the 3′ untranslated region of the Sfpi1 gene, located on mouse chromosome 2. The Sfpi1 gene is located in the AML minimal deleted region (mdr) between D2Mit126 and D2Mit185. Exons of the gene are denoted as boxes with the coding region in grey and introns as a black line, where exon 5 is specifically denoted. The direction of transcription is marked by arrows. Three primer binding sites forward A, reverse B and forward C are indicated. Stop: Stop codon, IRES: internal ribosome entry site, Neo: neomycin cassette.

Mentions: Both CBA/H Sfpi1GFP/GFP and CBA/H Sfpi1GFP/+ animals (generated from a homozygous male and wild type CBA/H female) were utilised in this study. All animals were bred and handled according to UK Home Office Animals (Scientific Procedures) Act 1986 and with guidance from the local ethical review committee on animal experiments. Details of the original construct, its localisation on mouse chromosome 2 and position of primers used for genotyping are presented in Fig. 1.


Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

Olme CH, Finnon R, Brown N, Kabacik S, Bouffler SD, Badie C - Leuk. Res. (2013)

Location of the GFP construct and a detailed view of the some of its features. The GFP construct is situated in the 3′ untranslated region of the Sfpi1 gene, located on mouse chromosome 2. The Sfpi1 gene is located in the AML minimal deleted region (mdr) between D2Mit126 and D2Mit185. Exons of the gene are denoted as boxes with the coding region in grey and introns as a black line, where exon 5 is specifically denoted. The direction of transcription is marked by arrows. Three primer binding sites forward A, reverse B and forward C are indicated. Stop: Stop codon, IRES: internal ribosome entry site, Neo: neomycin cassette.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3775122&req=5

fig0010: Location of the GFP construct and a detailed view of the some of its features. The GFP construct is situated in the 3′ untranslated region of the Sfpi1 gene, located on mouse chromosome 2. The Sfpi1 gene is located in the AML minimal deleted region (mdr) between D2Mit126 and D2Mit185. Exons of the gene are denoted as boxes with the coding region in grey and introns as a black line, where exon 5 is specifically denoted. The direction of transcription is marked by arrows. Three primer binding sites forward A, reverse B and forward C are indicated. Stop: Stop codon, IRES: internal ribosome entry site, Neo: neomycin cassette.
Mentions: Both CBA/H Sfpi1GFP/GFP and CBA/H Sfpi1GFP/+ animals (generated from a homozygous male and wild type CBA/H female) were utilised in this study. All animals were bred and handled according to UK Home Office Animals (Scientific Procedures) Act 1986 and with guidance from the local ethical review committee on animal experiments. Details of the original construct, its localisation on mouse chromosome 2 and position of primers used for genotyping are presented in Fig. 1.

Bottom Line: A specific interstitial deletion of chromosome 2 found in a high proportion of rAML is recognised as the initiating event.Although the deletion can be detected early following ionising radiation exposure by cytogenetic techniques, precise characterisation of the haematopoietic cells carrying the deletion and the study of their fate in vivo cannot be achieved.This study presents the first experimental evidence for the detection of this leukaemia initiating event in live leukemic cells.

View Article: PubMed Central - PubMed

Affiliation: Biological Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, Oxfordshire, UK.

Show MeSH
Related in: MedlinePlus