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S100A11 is a migration-related protein in laryngeal squamous cell carcinoma.

Wang C, Zhang Z, Li L, Zhang J, Wang J, Fan J, Jiao B, Zhao S - Int J Med Sci (2013)

Bottom Line: We found that both protein and mRNA levels of S100A11 were overexpressed in laryngeal tumor tissues when compared to the corresponding noncancerous tissues.Additionally, S100A11 altered a series of intracellular events, including the down-regulation of epidermal growth factor receptor (EGFR), CD44 and MMP2.These results highlight the significance of S100A11 in LSCC progression and suggest that the dysregulation of S100A11 might contribute to the metastatic progression of LSCC.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Otolaryngology-Head and Neck Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;

ABSTRACT

Objective: As a member of the S100 proteins family, the involvement of S100A11 has been suggested in a wide range of biological processes such as cell growth and motility, cell-cycle progression, transcription, differentiation and smooth muscle cell migration. However, the expression of S100A11 and its exact function in laryngeal squamous cell carcinoma (LSCC) have not been elucidated.

Methods: The protein and mRNA expression levels of S100A11 were analyzed in primary tumors and matched tumor-adjacent tissues of LSCC by western blotting and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) or quantitative real time PCR (Q-RT-PCR), respectively. Cell proliferation, colony formation, migration and wound-healing assays were performed to assess whether the knockdown of S100A11 by small interfering RNA (siRNA) could influence the biological behavior of human laryngeal carcinoma Hep-2 cells in vitro.

Results: We found that both protein and mRNA levels of S100A11 were overexpressed in laryngeal tumor tissues when compared to the corresponding noncancerous tissues. Further, it was demonstrated that the expression of S100A11 could induce migration but not proliferation of Hep-2 cells. Additionally, S100A11 altered a series of intracellular events, including the down-regulation of epidermal growth factor receptor (EGFR), CD44 and MMP2.

Conclusions: These results highlight the significance of S100A11 in LSCC progression and suggest that the dysregulation of S100A11 might contribute to the metastatic progression of LSCC.

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The effects of S100A11 silencing on the expression of migration related proteins in Hep-2 cells. A: The protein expression levels of EGFR, CD44 and MMP2 were analyzed by western blotting in Hep-2 cells that had been transfected with S100A11 siRNA. B: Histograms represent the intensities of the EGFR/GAPDH, CD44/GAPDH and MMP2/GAPDH protein expression ratios.
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Figure 5: The effects of S100A11 silencing on the expression of migration related proteins in Hep-2 cells. A: The protein expression levels of EGFR, CD44 and MMP2 were analyzed by western blotting in Hep-2 cells that had been transfected with S100A11 siRNA. B: Histograms represent the intensities of the EGFR/GAPDH, CD44/GAPDH and MMP2/GAPDH protein expression ratios.

Mentions: The epidermal growth factor receptor (EGFR), CD44 and MMP2 proteins play important roles in cancer cell invasion and metastasis 24-26. Therefore, we tested the EGFR, CD44 and MMP2 protein expression levels in Hep-2 cells that had been treated with S100A11 or negative control siRNA. The relative protein expression levels of EGFR, CD44 and MMP2 decreased 2.17-fold, 3.93-fold and 3.90-fold, respectively in the S100A11 siRNA group when compared to the levels in the negative control siRNA group (P<0.05; Figure 5). The conclusion was that S100A11 induced the downregulation of these migration-related proteins.


S100A11 is a migration-related protein in laryngeal squamous cell carcinoma.

Wang C, Zhang Z, Li L, Zhang J, Wang J, Fan J, Jiao B, Zhao S - Int J Med Sci (2013)

The effects of S100A11 silencing on the expression of migration related proteins in Hep-2 cells. A: The protein expression levels of EGFR, CD44 and MMP2 were analyzed by western blotting in Hep-2 cells that had been transfected with S100A11 siRNA. B: Histograms represent the intensities of the EGFR/GAPDH, CD44/GAPDH and MMP2/GAPDH protein expression ratios.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3775114&req=5

Figure 5: The effects of S100A11 silencing on the expression of migration related proteins in Hep-2 cells. A: The protein expression levels of EGFR, CD44 and MMP2 were analyzed by western blotting in Hep-2 cells that had been transfected with S100A11 siRNA. B: Histograms represent the intensities of the EGFR/GAPDH, CD44/GAPDH and MMP2/GAPDH protein expression ratios.
Mentions: The epidermal growth factor receptor (EGFR), CD44 and MMP2 proteins play important roles in cancer cell invasion and metastasis 24-26. Therefore, we tested the EGFR, CD44 and MMP2 protein expression levels in Hep-2 cells that had been treated with S100A11 or negative control siRNA. The relative protein expression levels of EGFR, CD44 and MMP2 decreased 2.17-fold, 3.93-fold and 3.90-fold, respectively in the S100A11 siRNA group when compared to the levels in the negative control siRNA group (P<0.05; Figure 5). The conclusion was that S100A11 induced the downregulation of these migration-related proteins.

Bottom Line: We found that both protein and mRNA levels of S100A11 were overexpressed in laryngeal tumor tissues when compared to the corresponding noncancerous tissues.Additionally, S100A11 altered a series of intracellular events, including the down-regulation of epidermal growth factor receptor (EGFR), CD44 and MMP2.These results highlight the significance of S100A11 in LSCC progression and suggest that the dysregulation of S100A11 might contribute to the metastatic progression of LSCC.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Otolaryngology-Head and Neck Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;

ABSTRACT

Objective: As a member of the S100 proteins family, the involvement of S100A11 has been suggested in a wide range of biological processes such as cell growth and motility, cell-cycle progression, transcription, differentiation and smooth muscle cell migration. However, the expression of S100A11 and its exact function in laryngeal squamous cell carcinoma (LSCC) have not been elucidated.

Methods: The protein and mRNA expression levels of S100A11 were analyzed in primary tumors and matched tumor-adjacent tissues of LSCC by western blotting and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) or quantitative real time PCR (Q-RT-PCR), respectively. Cell proliferation, colony formation, migration and wound-healing assays were performed to assess whether the knockdown of S100A11 by small interfering RNA (siRNA) could influence the biological behavior of human laryngeal carcinoma Hep-2 cells in vitro.

Results: We found that both protein and mRNA levels of S100A11 were overexpressed in laryngeal tumor tissues when compared to the corresponding noncancerous tissues. Further, it was demonstrated that the expression of S100A11 could induce migration but not proliferation of Hep-2 cells. Additionally, S100A11 altered a series of intracellular events, including the down-regulation of epidermal growth factor receptor (EGFR), CD44 and MMP2.

Conclusions: These results highlight the significance of S100A11 in LSCC progression and suggest that the dysregulation of S100A11 might contribute to the metastatic progression of LSCC.

Show MeSH
Related in: MedlinePlus