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Analysis of immunoglobulin and T cell receptor gene rearrangement in the bone marrow of lymphoid neoplasia using BIOMED-2 multiplex polymerase chain reaction.

Shin S, Kim AH, Park J, Kim M, Lim J, Kim Y, Han K, Lee SA, Cho SG - Int J Med Sci (2013)

Bottom Line: We evaluated of immunoglobulin (Ig) and/or T-cell receptor (TCR) gene rearrangements in the BM using the standardized BIOMED-2 multiplex PCR clonality assays and compared the results with microscopic findings such as histology and CD10, CD20, CD79a, CD3 and CD5 immunohistochemistry.Two cases among them turned into microscopic BM involvement during a close follow up.Therefore, comprehensive analysis with morphologic analysis should be important to reach a final diagnosis.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine, Seoul, Korea;

ABSTRACT
The evaluation of bone marrow (BM) involvement is important for diagnosis and staging in patients with lymphoid neoplasia. We evaluated of immunoglobulin (Ig) and/or T-cell receptor (TCR) gene rearrangements in the BM using the standardized BIOMED-2 multiplex PCR clonality assays and compared the results with microscopic findings such as histology and CD10, CD20, CD79a, CD3 and CD5 immunohistochemistry. A total of 151 samples were enrolled; 119 B cell neoplasia, 29 T cell neoplasia, and 3 Hodgkin's lymphoma. The molecular clonality assay and microscopic diagnosis were concordant in 66.9% (n=101) and discordant in 33.1 % (n=50). Ig/TCR gene clonality assay detected 43 cases of BM involvement which was not presented in the morphology. Two cases among them turned into microscopic BM involvement during a close follow up. Clonal TCR gene rearrangements were detected in 12.6% of B cell neoplasia and Ig gene rearrangement were found in 3.4% of T cell neoplasia. This molecular clonality assay is valuable particularly in diagnosing BM involvement of lymphoid neoplasia if it is morphologically uncertain. But it should be carefully interpreted because molecular clonality may be present in the reactive lymphoproliferation. Therefore, comprehensive analysis with morphologic analysis should be important to reach a final diagnosis.

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Related in: MedlinePlus

Examples of cases with identical clones in the bone marrow (top) and lymphoma tissue(bottom). Clonal analysis of TCRBA of T lymphoblastic lymphoma (a) and IGHA of mantle cell lymphoma(b).
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Figure 1: Examples of cases with identical clones in the bone marrow (top) and lymphoma tissue(bottom). Clonal analysis of TCRBA of T lymphoblastic lymphoma (a) and IGHA of mantle cell lymphoma(b).

Mentions: We compared BM Ig/TCR gene rearrangement results with the molecular results of the original lymphoma tissues in some cases and showed the profiles in Fig.1.


Analysis of immunoglobulin and T cell receptor gene rearrangement in the bone marrow of lymphoid neoplasia using BIOMED-2 multiplex polymerase chain reaction.

Shin S, Kim AH, Park J, Kim M, Lim J, Kim Y, Han K, Lee SA, Cho SG - Int J Med Sci (2013)

Examples of cases with identical clones in the bone marrow (top) and lymphoma tissue(bottom). Clonal analysis of TCRBA of T lymphoblastic lymphoma (a) and IGHA of mantle cell lymphoma(b).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3775108&req=5

Figure 1: Examples of cases with identical clones in the bone marrow (top) and lymphoma tissue(bottom). Clonal analysis of TCRBA of T lymphoblastic lymphoma (a) and IGHA of mantle cell lymphoma(b).
Mentions: We compared BM Ig/TCR gene rearrangement results with the molecular results of the original lymphoma tissues in some cases and showed the profiles in Fig.1.

Bottom Line: We evaluated of immunoglobulin (Ig) and/or T-cell receptor (TCR) gene rearrangements in the BM using the standardized BIOMED-2 multiplex PCR clonality assays and compared the results with microscopic findings such as histology and CD10, CD20, CD79a, CD3 and CD5 immunohistochemistry.Two cases among them turned into microscopic BM involvement during a close follow up.Therefore, comprehensive analysis with morphologic analysis should be important to reach a final diagnosis.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine, Seoul, Korea;

ABSTRACT
The evaluation of bone marrow (BM) involvement is important for diagnosis and staging in patients with lymphoid neoplasia. We evaluated of immunoglobulin (Ig) and/or T-cell receptor (TCR) gene rearrangements in the BM using the standardized BIOMED-2 multiplex PCR clonality assays and compared the results with microscopic findings such as histology and CD10, CD20, CD79a, CD3 and CD5 immunohistochemistry. A total of 151 samples were enrolled; 119 B cell neoplasia, 29 T cell neoplasia, and 3 Hodgkin's lymphoma. The molecular clonality assay and microscopic diagnosis were concordant in 66.9% (n=101) and discordant in 33.1 % (n=50). Ig/TCR gene clonality assay detected 43 cases of BM involvement which was not presented in the morphology. Two cases among them turned into microscopic BM involvement during a close follow up. Clonal TCR gene rearrangements were detected in 12.6% of B cell neoplasia and Ig gene rearrangement were found in 3.4% of T cell neoplasia. This molecular clonality assay is valuable particularly in diagnosing BM involvement of lymphoid neoplasia if it is morphologically uncertain. But it should be carefully interpreted because molecular clonality may be present in the reactive lymphoproliferation. Therefore, comprehensive analysis with morphologic analysis should be important to reach a final diagnosis.

Show MeSH
Related in: MedlinePlus