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The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

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Real-time qPCR analysis of the expression patterns of ten genes related to pathogenesis pathway in the wild-type Yangmai 158 and Hv-SGT1-over-expressing transgenic plants (OX-323) before inoculation of Bgt and 24 h post inoculation with Bgt.* p < 0.05, ** p < 0.01 compared with the wild type. Over-expression Hv-SGT1 regulates the expression of genes related to the H2O2, SA and JA pathways.
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pone-0072571-g010: Real-time qPCR analysis of the expression patterns of ten genes related to pathogenesis pathway in the wild-type Yangmai 158 and Hv-SGT1-over-expressing transgenic plants (OX-323) before inoculation of Bgt and 24 h post inoculation with Bgt.* p < 0.05, ** p < 0.01 compared with the wild type. Over-expression Hv-SGT1 regulates the expression of genes related to the H2O2, SA and JA pathways.

Mentions: The H2O2-producing gene PR9 was significantly up-regulated in the transgenic plants after Bgt inoculation. Interestingly, another gene responsible for H2O2 production, NADPHOX, was down-regulated following inoculation with Bgt. The H2O2-scavenging gene, APX, was significantly up-regulated in the transgenic plants after inoculation with Bgt, and the CAT gene, which has a similar function, was only slightly up-regulated. Unlike enzymatic antioxidative system genes, GST (marker gene of non-enzymatic antioxidative system) was significantly down-regulated in the transgenic plants after inoculation with Bgt, but the GPOX gene, which has a similar function, was not regulated by SGT1 (Figure 10). After Bgt inoculation, the expression levels of genes related to the JA-signaling pathway, including those involved in JA synthesis (OPDA), JA signal transduction (COI1 and ERF), and response to the JA signal (PR-10) were substantially higher (Figure 10) in OX-323 transgenic plants than in WT plats after inoculation with Bgt. However, expression of marker genes of the SA pathway, such as NPR1, PR5 andPR2, was higher in the transgenic plants than in the WT plants, but down-regulated after inoculation with Bgt (Figure 10). Meanwhile, we found expression of PR3 (marker gene of ET pathway) was hardly changed in the transgenic plants both before and after inoculation with Bgt. We presumed that the over-expression of Hv-SGT1 might regulate the balance between the production and scavenging of H2O2, stimulate JA signaling, and suppress the SA defense pathway, but was not directly involved in the ET defense pathway.


The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Real-time qPCR analysis of the expression patterns of ten genes related to pathogenesis pathway in the wild-type Yangmai 158 and Hv-SGT1-over-expressing transgenic plants (OX-323) before inoculation of Bgt and 24 h post inoculation with Bgt.* p < 0.05, ** p < 0.01 compared with the wild type. Over-expression Hv-SGT1 regulates the expression of genes related to the H2O2, SA and JA pathways.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3760960&req=5

pone-0072571-g010: Real-time qPCR analysis of the expression patterns of ten genes related to pathogenesis pathway in the wild-type Yangmai 158 and Hv-SGT1-over-expressing transgenic plants (OX-323) before inoculation of Bgt and 24 h post inoculation with Bgt.* p < 0.05, ** p < 0.01 compared with the wild type. Over-expression Hv-SGT1 regulates the expression of genes related to the H2O2, SA and JA pathways.
Mentions: The H2O2-producing gene PR9 was significantly up-regulated in the transgenic plants after Bgt inoculation. Interestingly, another gene responsible for H2O2 production, NADPHOX, was down-regulated following inoculation with Bgt. The H2O2-scavenging gene, APX, was significantly up-regulated in the transgenic plants after inoculation with Bgt, and the CAT gene, which has a similar function, was only slightly up-regulated. Unlike enzymatic antioxidative system genes, GST (marker gene of non-enzymatic antioxidative system) was significantly down-regulated in the transgenic plants after inoculation with Bgt, but the GPOX gene, which has a similar function, was not regulated by SGT1 (Figure 10). After Bgt inoculation, the expression levels of genes related to the JA-signaling pathway, including those involved in JA synthesis (OPDA), JA signal transduction (COI1 and ERF), and response to the JA signal (PR-10) were substantially higher (Figure 10) in OX-323 transgenic plants than in WT plats after inoculation with Bgt. However, expression of marker genes of the SA pathway, such as NPR1, PR5 andPR2, was higher in the transgenic plants than in the WT plants, but down-regulated after inoculation with Bgt (Figure 10). Meanwhile, we found expression of PR3 (marker gene of ET pathway) was hardly changed in the transgenic plants both before and after inoculation with Bgt. We presumed that the over-expression of Hv-SGT1 might regulate the balance between the production and scavenging of H2O2, stimulate JA signaling, and suppress the SA defense pathway, but was not directly involved in the ET defense pathway.

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

Show MeSH
Related in: MedlinePlus