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The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

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Relative expression levels of Ta-SGT1 in spikes of scab-resistant wheat variety Wangshuibai (WSB) and its susceptible mutant NAUH117 at different times after inoculation with Fg.(A) Ta-SGT1 was constitutively expressed in both WSB and NAUH117, and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. (B) The FHB symptom of spikes of Yangmai158 and transgenic plants at 21 days after Fusarium inoculation, scale bar represents 1cm.
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pone-0072571-g009: Relative expression levels of Ta-SGT1 in spikes of scab-resistant wheat variety Wangshuibai (WSB) and its susceptible mutant NAUH117 at different times after inoculation with Fg.(A) Ta-SGT1 was constitutively expressed in both WSB and NAUH117, and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. (B) The FHB symptom of spikes of Yangmai158 and transgenic plants at 21 days after Fusarium inoculation, scale bar represents 1cm.

Mentions: Unlike the inducible expression pattern of Hv-SGT1 in spikes of H. villosa after inoculation with Fg, Ta-SGT1 was constitutively expressed in both the FHB-resistant Wangshuibai (WSB) cultivar and its susceptible mutant NAUH117 (Figure 9A), and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. To determine whether higher expression levels of SGT1 was required for the resistance to Fg, we further investigated the over-expression plants transformed to express Hv-SGT1 under the regulation of the CAMV35S promoter for resistance to infection by the hemi-biotrophic Fg.


The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Relative expression levels of Ta-SGT1 in spikes of scab-resistant wheat variety Wangshuibai (WSB) and its susceptible mutant NAUH117 at different times after inoculation with Fg.(A) Ta-SGT1 was constitutively expressed in both WSB and NAUH117, and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. (B) The FHB symptom of spikes of Yangmai158 and transgenic plants at 21 days after Fusarium inoculation, scale bar represents 1cm.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3760960&req=5

pone-0072571-g009: Relative expression levels of Ta-SGT1 in spikes of scab-resistant wheat variety Wangshuibai (WSB) and its susceptible mutant NAUH117 at different times after inoculation with Fg.(A) Ta-SGT1 was constitutively expressed in both WSB and NAUH117, and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. (B) The FHB symptom of spikes of Yangmai158 and transgenic plants at 21 days after Fusarium inoculation, scale bar represents 1cm.
Mentions: Unlike the inducible expression pattern of Hv-SGT1 in spikes of H. villosa after inoculation with Fg, Ta-SGT1 was constitutively expressed in both the FHB-resistant Wangshuibai (WSB) cultivar and its susceptible mutant NAUH117 (Figure 9A), and no obvious change was detected after inoculation with Fg. Nonetheless, an approximately 6-fold lower expression level of Ta-SGT1 transcript was detected in the susceptible mutant NAUH117 than that in resistant WSB. To determine whether higher expression levels of SGT1 was required for the resistance to Fg, we further investigated the over-expression plants transformed to express Hv-SGT1 under the regulation of the CAMV35S promoter for resistance to infection by the hemi-biotrophic Fg.

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

Show MeSH
Related in: MedlinePlus