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The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

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Responses of SGT1 to treatments of biotic and abiotic stresses, as well as phytohormone applications.Expression patterns of SGT1 (A) in different organs of H. villosa, (B) in H. villosa leaves after Bgt inoculation, (C) in H. villosa immature spikes after Fg inoculation, and (D) in H. villosa leaves fter treatments with ET, ABA, SA, MeJA, and H2O2.
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pone-0072571-g004: Responses of SGT1 to treatments of biotic and abiotic stresses, as well as phytohormone applications.Expression patterns of SGT1 (A) in different organs of H. villosa, (B) in H. villosa leaves after Bgt inoculation, (C) in H. villosa immature spikes after Fg inoculation, and (D) in H. villosa leaves fter treatments with ET, ABA, SA, MeJA, and H2O2.

Mentions: Expression profiling of Hv-SGT1 using different tissues of H. villosa showed that the highest expression was observed in leaves and immature spikes, with lower levels of expression observed in stems and roots (Figure 4A). Expression of the Hv-SGT1 gene was rapidly induced during infection of the leaves of seedlings of Bgt-resistant H. villosa, with levels of transcripts peaking 12 h after infection (Figure 4B). Similar inducible expression patterns were also observed in the immature spike of H. villosa after infection with Fg (Figure 4C). Treatment of the leaves of H. villosa seedlings with different phytohormones revealed that Hv-SGT1 transcripts were strongly induced by H2O2 and methyl jasmonate (MeJA), slightly induced by ethepon (ET) and abscisic acid (ABA), but not by salicylic acid (SA) (Figure 4D). The inducible expression of Hv-SGT1 following pathogen infection and treatment with phytohormones suggested its involvement in disease resistance reactions and defense pathways.


The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Responses of SGT1 to treatments of biotic and abiotic stresses, as well as phytohormone applications.Expression patterns of SGT1 (A) in different organs of H. villosa, (B) in H. villosa leaves after Bgt inoculation, (C) in H. villosa immature spikes after Fg inoculation, and (D) in H. villosa leaves fter treatments with ET, ABA, SA, MeJA, and H2O2.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3760960&req=5

pone-0072571-g004: Responses of SGT1 to treatments of biotic and abiotic stresses, as well as phytohormone applications.Expression patterns of SGT1 (A) in different organs of H. villosa, (B) in H. villosa leaves after Bgt inoculation, (C) in H. villosa immature spikes after Fg inoculation, and (D) in H. villosa leaves fter treatments with ET, ABA, SA, MeJA, and H2O2.
Mentions: Expression profiling of Hv-SGT1 using different tissues of H. villosa showed that the highest expression was observed in leaves and immature spikes, with lower levels of expression observed in stems and roots (Figure 4A). Expression of the Hv-SGT1 gene was rapidly induced during infection of the leaves of seedlings of Bgt-resistant H. villosa, with levels of transcripts peaking 12 h after infection (Figure 4B). Similar inducible expression patterns were also observed in the immature spike of H. villosa after infection with Fg (Figure 4C). Treatment of the leaves of H. villosa seedlings with different phytohormones revealed that Hv-SGT1 transcripts were strongly induced by H2O2 and methyl jasmonate (MeJA), slightly induced by ethepon (ET) and abscisic acid (ABA), but not by salicylic acid (SA) (Figure 4D). The inducible expression of Hv-SGT1 following pathogen infection and treatment with phytohormones suggested its involvement in disease resistance reactions and defense pathways.

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

Show MeSH
Related in: MedlinePlus