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The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

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Related in: MedlinePlus

Chromosomal location of Hv-SGT1 determined using the wheat–H. villosa addition lines as the templates.The arrow shows the specific amplicon from H. villosa.. M: DNA marker DL2000. HV:H. villosa, CS: wheat cv. Chinese spring, ABV: T. durum-H. villosa amphiploid, 2n=42, genome AABBVV, Add1V to Add7V: T. aestivum-H. villosa addition line, each contains one pair of chromosomes of H. villosa from 1V to 7V in the common wheat background, 2n=44, genome AABBDD add 1V1V~7V7V.
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pone-0072571-g002: Chromosomal location of Hv-SGT1 determined using the wheat–H. villosa addition lines as the templates.The arrow shows the specific amplicon from H. villosa.. M: DNA marker DL2000. HV:H. villosa, CS: wheat cv. Chinese spring, ABV: T. durum-H. villosa amphiploid, 2n=42, genome AABBVV, Add1V to Add7V: T. aestivum-H. villosa addition line, each contains one pair of chromosomes of H. villosa from 1V to 7V in the common wheat background, 2n=44, genome AABBDD add 1V1V~7V7V.

Mentions: The chromosomal location of Hv-SGT1 was determined following PCR-based amplification using DNA from the wheat-H. villosa addition lines as template. A 402-bp product was found in H. villosa, a T. durum–H. villosa amphiploid, and a wheat-H. villosa addition line DA3V, but not in Chinese spring and the remaining addition lines tested. This indicated that Hv-SGT1 was located on chromosome 3V (Figure 2).


The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.).

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M, Jin X, Hu J, Zhang Y, Wang X, Chen P - PLoS ONE (2013)

Chromosomal location of Hv-SGT1 determined using the wheat–H. villosa addition lines as the templates.The arrow shows the specific amplicon from H. villosa.. M: DNA marker DL2000. HV:H. villosa, CS: wheat cv. Chinese spring, ABV: T. durum-H. villosa amphiploid, 2n=42, genome AABBVV, Add1V to Add7V: T. aestivum-H. villosa addition line, each contains one pair of chromosomes of H. villosa from 1V to 7V in the common wheat background, 2n=44, genome AABBDD add 1V1V~7V7V.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3760960&req=5

pone-0072571-g002: Chromosomal location of Hv-SGT1 determined using the wheat–H. villosa addition lines as the templates.The arrow shows the specific amplicon from H. villosa.. M: DNA marker DL2000. HV:H. villosa, CS: wheat cv. Chinese spring, ABV: T. durum-H. villosa amphiploid, 2n=42, genome AABBVV, Add1V to Add7V: T. aestivum-H. villosa addition line, each contains one pair of chromosomes of H. villosa from 1V to 7V in the common wheat background, 2n=44, genome AABBDD add 1V1V~7V7V.
Mentions: The chromosomal location of Hv-SGT1 was determined following PCR-based amplification using DNA from the wheat-H. villosa addition lines as template. A 402-bp product was found in H. villosa, a T. durum–H. villosa amphiploid, and a wheat-H. villosa addition line DA3V, but not in Chinese spring and the remaining addition lines tested. This indicated that Hv-SGT1 was located on chromosome 3V (Figure 2).

Bottom Line: The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa.Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling.Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

View Article: PubMed Central - PubMed

Affiliation: The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldiavillosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeriagraminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusariumgraminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance.

Show MeSH
Related in: MedlinePlus