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Circulating microparticles from Crohn's disease patients cause endothelial and vascular dysfunctions.

Leonetti D, Reimund JM, Tesse A, Viennot S, Martinez MC, Bretagne AL, Andriantsitohaina R - PLoS ONE (2013)

Bottom Line: Circulating MP levels did not differ between HS and inactive CD patients except for an increase in leukocyte-derived MPs in CD.A significant correlation was found between total levels of MPs, those from platelets and endothelial cells, and the Harvey-Bradshaw clinical activity index.CDMPs induced vascular hypo-reactivity in aorta that was prevented by a nitric oxide (NO)-synthase inhibitor, and was associated with a subtle alteration of the balance between NO, reactive oxygen species and the release of COX metabolites.

View Article: PubMed Central - PubMed

Affiliation: LUNAM Université, Angers, France ; INSERM, U1063, Angers, France.

ABSTRACT

Background: Microparticles (MPs) are small vesicles released during cell activation or apoptosis. They are involved in coagulation, inflammation and vascular dysfunction in several diseases. We characterized circulating MPs from Crohn's Disease (CD) patients and evaluated their effects on endothelial function and vascular reactivity after in vivo injection into mice.

Methods: Circulating MPs and their cellular origins were examined by flow cytometry from blood samples from healthy subjects (HS) and inactive or active CD patients. MPs were intravenously injected into mice. After 24 hours, endothelial function and vascular reactivity were assessed.

Results: Circulating MP levels did not differ between HS and inactive CD patients except for an increase in leukocyte-derived MPs in CD. Active CD patients compared to HS displayed increased total circulating MPs, pro-coagulant MPs and those from platelets, endothelium, erythrocytes, leukocytes, activated leukocytes and activated platelets. A significant correlation was found between total levels of MPs, those from platelets and endothelial cells, and the Harvey-Bradshaw clinical activity index. MPs from CD, but not from HS, impaired endothelium-dependent relaxation in mice aorta and flow-induced dilation in mice small mesenteric arteries, MPs from inactive CD patients being more effective than those from active patients. CDMPs induced vascular hypo-reactivity in aorta that was prevented by a nitric oxide (NO)-synthase inhibitor, and was associated with a subtle alteration of the balance between NO, reactive oxygen species and the release of COX metabolites.

Conclusions: We provide evidence that MPs from CD patients significantly alter endothelial and vascular function and therefore, may play a role in CD pathophysiology, at least by contributing to uncontrolled vascular-dependent intestinal damage.

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Related in: MedlinePlus

Correlations between microparticle (MP) levels and disease activity.Correlations between total circulating MPs, CD41+ MPs, CD146+ MPs and Harvey-Bradshaw index (A–C).
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pone-0073088-g002: Correlations between microparticle (MP) levels and disease activity.Correlations between total circulating MPs, CD41+ MPs, CD146+ MPs and Harvey-Bradshaw index (A–C).

Mentions: Total MP concentration was not correlated to disease duration (r’ = 0.104, p = 0.55). Correlation analysis for disease activity showed that total circulating MPs and those from platelets and endothelial cells were positively correlated to the Harvey-Bradshaw index (Figure 2A–2C).


Circulating microparticles from Crohn's disease patients cause endothelial and vascular dysfunctions.

Leonetti D, Reimund JM, Tesse A, Viennot S, Martinez MC, Bretagne AL, Andriantsitohaina R - PLoS ONE (2013)

Correlations between microparticle (MP) levels and disease activity.Correlations between total circulating MPs, CD41+ MPs, CD146+ MPs and Harvey-Bradshaw index (A–C).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3760904&req=5

pone-0073088-g002: Correlations between microparticle (MP) levels and disease activity.Correlations between total circulating MPs, CD41+ MPs, CD146+ MPs and Harvey-Bradshaw index (A–C).
Mentions: Total MP concentration was not correlated to disease duration (r’ = 0.104, p = 0.55). Correlation analysis for disease activity showed that total circulating MPs and those from platelets and endothelial cells were positively correlated to the Harvey-Bradshaw index (Figure 2A–2C).

Bottom Line: Circulating MP levels did not differ between HS and inactive CD patients except for an increase in leukocyte-derived MPs in CD.A significant correlation was found between total levels of MPs, those from platelets and endothelial cells, and the Harvey-Bradshaw clinical activity index.CDMPs induced vascular hypo-reactivity in aorta that was prevented by a nitric oxide (NO)-synthase inhibitor, and was associated with a subtle alteration of the balance between NO, reactive oxygen species and the release of COX metabolites.

View Article: PubMed Central - PubMed

Affiliation: LUNAM Université, Angers, France ; INSERM, U1063, Angers, France.

ABSTRACT

Background: Microparticles (MPs) are small vesicles released during cell activation or apoptosis. They are involved in coagulation, inflammation and vascular dysfunction in several diseases. We characterized circulating MPs from Crohn's Disease (CD) patients and evaluated their effects on endothelial function and vascular reactivity after in vivo injection into mice.

Methods: Circulating MPs and their cellular origins were examined by flow cytometry from blood samples from healthy subjects (HS) and inactive or active CD patients. MPs were intravenously injected into mice. After 24 hours, endothelial function and vascular reactivity were assessed.

Results: Circulating MP levels did not differ between HS and inactive CD patients except for an increase in leukocyte-derived MPs in CD. Active CD patients compared to HS displayed increased total circulating MPs, pro-coagulant MPs and those from platelets, endothelium, erythrocytes, leukocytes, activated leukocytes and activated platelets. A significant correlation was found between total levels of MPs, those from platelets and endothelial cells, and the Harvey-Bradshaw clinical activity index. MPs from CD, but not from HS, impaired endothelium-dependent relaxation in mice aorta and flow-induced dilation in mice small mesenteric arteries, MPs from inactive CD patients being more effective than those from active patients. CDMPs induced vascular hypo-reactivity in aorta that was prevented by a nitric oxide (NO)-synthase inhibitor, and was associated with a subtle alteration of the balance between NO, reactive oxygen species and the release of COX metabolites.

Conclusions: We provide evidence that MPs from CD patients significantly alter endothelial and vascular function and therefore, may play a role in CD pathophysiology, at least by contributing to uncontrolled vascular-dependent intestinal damage.

Show MeSH
Related in: MedlinePlus