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Recent Advance in Biosensors for microRNAs Detection in Cancer.

Catuogno S, Esposito CL, Quintavalle C, Cerchia L, Condorelli G, De Franciscis V - Cancers (Basel) (2011)

Bottom Line: MicroRNAs (miRNAs) are short non-protein-coding RNA molecules that regulate the expression of a wide variety of genes.Recent studies have implicated miRNAs in a wide range of biological processes and diseases including development, metabolism and cancer, and revealed that expression levels of individual miRNAs may serve as reliable molecular biomarkers for cancer diagnosis and prognosis.Therefore, a major challenge is to develop innovative tools able to couple high sensitivity and specificity for rapid detection of miRNAs in a given cell or tissue.

View Article: PubMed Central - PubMed

Affiliation: Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR ''G. Salvatore'', Via S. Pansini 5, 80131 Naples, Italy. defranci@unina.it.

ABSTRACT
MicroRNAs (miRNAs) are short non-protein-coding RNA molecules that regulate the expression of a wide variety of genes. They act by sequence-specific base pairing in the 3' untranslated region (3'UTR) of the target mRNA leading to mRNA degradation or translation inhibition. Recent studies have implicated miRNAs in a wide range of biological processes and diseases including development, metabolism and cancer, and revealed that expression levels of individual miRNAs may serve as reliable molecular biomarkers for cancer diagnosis and prognosis. Therefore, a major challenge is to develop innovative tools able to couple high sensitivity and specificity for rapid detection of miRNAs in a given cell or tissue. In this review, we focus on the latest innovative approaches proposed for miRNA profiling in cancer and discuss their advantages and disadvantages.

No MeSH data available.


Related in: MedlinePlus

Main methods used for circulating miRNA detection.
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f2-cancers-03-01877: Main methods used for circulating miRNA detection.

Mentions: Recently, circulating miRNAs are emerging as very promising biomarkers for cancer [29,30], since they are abundant, tissue specific and relatively stable. Thus, several methods have been recently proposed to detect miRNAs in serum, plasma, urine and other human body fluids (Figure 2). Noteworthy the use of body fluids as biological materials for miRNA assays has the main advantage to be noninvasive or minimally invasive approaches.


Recent Advance in Biosensors for microRNAs Detection in Cancer.

Catuogno S, Esposito CL, Quintavalle C, Cerchia L, Condorelli G, De Franciscis V - Cancers (Basel) (2011)

Main methods used for circulating miRNA detection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3757395&req=5

f2-cancers-03-01877: Main methods used for circulating miRNA detection.
Mentions: Recently, circulating miRNAs are emerging as very promising biomarkers for cancer [29,30], since they are abundant, tissue specific and relatively stable. Thus, several methods have been recently proposed to detect miRNAs in serum, plasma, urine and other human body fluids (Figure 2). Noteworthy the use of body fluids as biological materials for miRNA assays has the main advantage to be noninvasive or minimally invasive approaches.

Bottom Line: MicroRNAs (miRNAs) are short non-protein-coding RNA molecules that regulate the expression of a wide variety of genes.Recent studies have implicated miRNAs in a wide range of biological processes and diseases including development, metabolism and cancer, and revealed that expression levels of individual miRNAs may serve as reliable molecular biomarkers for cancer diagnosis and prognosis.Therefore, a major challenge is to develop innovative tools able to couple high sensitivity and specificity for rapid detection of miRNAs in a given cell or tissue.

View Article: PubMed Central - PubMed

Affiliation: Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR ''G. Salvatore'', Via S. Pansini 5, 80131 Naples, Italy. defranci@unina.it.

ABSTRACT
MicroRNAs (miRNAs) are short non-protein-coding RNA molecules that regulate the expression of a wide variety of genes. They act by sequence-specific base pairing in the 3' untranslated region (3'UTR) of the target mRNA leading to mRNA degradation or translation inhibition. Recent studies have implicated miRNAs in a wide range of biological processes and diseases including development, metabolism and cancer, and revealed that expression levels of individual miRNAs may serve as reliable molecular biomarkers for cancer diagnosis and prognosis. Therefore, a major challenge is to develop innovative tools able to couple high sensitivity and specificity for rapid detection of miRNAs in a given cell or tissue. In this review, we focus on the latest innovative approaches proposed for miRNA profiling in cancer and discuss their advantages and disadvantages.

No MeSH data available.


Related in: MedlinePlus