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Magnetoresistive emulsion analyzer.

Lin G, Baraban L, Han L, Karnaushenko D, Makarov D, Cuniberti G, Schmidt OG - Sci Rep (2013)

Bottom Line: We realize a magnetoresistive emulsion analyzer capable of detection, multiparametric analysis and sorting of ferrofluid-containing nanoliter-droplets.The operation of the device in a cytometric mode provides high throughput and quantitative information about the dimensions and magnetic content of the emulsion.Our method offers important complementarity to conventional optical approaches involving ferrofluids, and paves the way to the development of novel compact tools for diagnostics and nanomedicine including drug design and screening.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstrasse 20, Dresden, Germany.

ABSTRACT
We realize a magnetoresistive emulsion analyzer capable of detection, multiparametric analysis and sorting of ferrofluid-containing nanoliter-droplets. The operation of the device in a cytometric mode provides high throughput and quantitative information about the dimensions and magnetic content of the emulsion. Our method offers important complementarity to conventional optical approaches involving ferrofluids, and paves the way to the development of novel compact tools for diagnostics and nanomedicine including drug design and screening.

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Multiparametric density plots for the detection of magnetic droplets which are produced with (a) different volume of ferrofluid droplets (concentration: 15 mg/ml) and (b) different concentrations of ferrofluid in droplets (volume: 160 nl). The flow rate of mineral oil is Qoil  = 500 nl/s. Circles are guide to the eyes. Histograms of the voltage amplitude change with (c) different volume of ferrofluid droplets and (d) different concentrations of ferrofluid in droplets. These plots collect measurement data of about 100 droplets.
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f2: Multiparametric density plots for the detection of magnetic droplets which are produced with (a) different volume of ferrofluid droplets (concentration: 15 mg/ml) and (b) different concentrations of ferrofluid in droplets (volume: 160 nl). The flow rate of mineral oil is Qoil = 500 nl/s. Circles are guide to the eyes. Histograms of the voltage amplitude change with (c) different volume of ferrofluid droplets and (d) different concentrations of ferrofluid in droplets. These plots collect measurement data of about 100 droplets.

Mentions: The multiparametric density plots produced for different volumes of ferrofluid droplets (concentration: 15 mg/ml) are summarized in Figure 2a. The broad distribution of the FWHM and amplitude for the smallest droplets (black cloud, Fig. 2a) are consistent with the real time detection results (Supplementary Figure 1a). The corresponding broad standard deviations of the fitted histograms in Figure 2c reflect predominantly the clear polydispersity of droplet sizes, and is to lesser extent ascribed to the inhomogeneity of the ferrofluid concentration between droplets. The polydispersity is partly related to the spontaneous fusion of droplets smaller than the channel dimension. With increasing size (compare red and green clouds), the droplets completely fill the channel cross-section resulting in a more uniform droplet train as reflected by the narrower distribution of the scattered data points around the cloud center (Supplementary Figure 2i). Once the droplet is longer than the width of the magnetic sensor, the sensor voltage change (amplitude) reaches its saturation value and becomes insensitive to the size of the droplets. However, the information about the size of droplets can still be extracted from the FWHM (compare green and blue clouds, Figure 2a). An increase of the FWHM magnitude is observed when the droplet volume increases from 120 nl to 270 nl.


Magnetoresistive emulsion analyzer.

Lin G, Baraban L, Han L, Karnaushenko D, Makarov D, Cuniberti G, Schmidt OG - Sci Rep (2013)

Multiparametric density plots for the detection of magnetic droplets which are produced with (a) different volume of ferrofluid droplets (concentration: 15 mg/ml) and (b) different concentrations of ferrofluid in droplets (volume: 160 nl). The flow rate of mineral oil is Qoil  = 500 nl/s. Circles are guide to the eyes. Histograms of the voltage amplitude change with (c) different volume of ferrofluid droplets and (d) different concentrations of ferrofluid in droplets. These plots collect measurement data of about 100 droplets.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3757360&req=5

f2: Multiparametric density plots for the detection of magnetic droplets which are produced with (a) different volume of ferrofluid droplets (concentration: 15 mg/ml) and (b) different concentrations of ferrofluid in droplets (volume: 160 nl). The flow rate of mineral oil is Qoil = 500 nl/s. Circles are guide to the eyes. Histograms of the voltage amplitude change with (c) different volume of ferrofluid droplets and (d) different concentrations of ferrofluid in droplets. These plots collect measurement data of about 100 droplets.
Mentions: The multiparametric density plots produced for different volumes of ferrofluid droplets (concentration: 15 mg/ml) are summarized in Figure 2a. The broad distribution of the FWHM and amplitude for the smallest droplets (black cloud, Fig. 2a) are consistent with the real time detection results (Supplementary Figure 1a). The corresponding broad standard deviations of the fitted histograms in Figure 2c reflect predominantly the clear polydispersity of droplet sizes, and is to lesser extent ascribed to the inhomogeneity of the ferrofluid concentration between droplets. The polydispersity is partly related to the spontaneous fusion of droplets smaller than the channel dimension. With increasing size (compare red and green clouds), the droplets completely fill the channel cross-section resulting in a more uniform droplet train as reflected by the narrower distribution of the scattered data points around the cloud center (Supplementary Figure 2i). Once the droplet is longer than the width of the magnetic sensor, the sensor voltage change (amplitude) reaches its saturation value and becomes insensitive to the size of the droplets. However, the information about the size of droplets can still be extracted from the FWHM (compare green and blue clouds, Figure 2a). An increase of the FWHM magnitude is observed when the droplet volume increases from 120 nl to 270 nl.

Bottom Line: We realize a magnetoresistive emulsion analyzer capable of detection, multiparametric analysis and sorting of ferrofluid-containing nanoliter-droplets.The operation of the device in a cytometric mode provides high throughput and quantitative information about the dimensions and magnetic content of the emulsion.Our method offers important complementarity to conventional optical approaches involving ferrofluids, and paves the way to the development of novel compact tools for diagnostics and nanomedicine including drug design and screening.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstrasse 20, Dresden, Germany.

ABSTRACT
We realize a magnetoresistive emulsion analyzer capable of detection, multiparametric analysis and sorting of ferrofluid-containing nanoliter-droplets. The operation of the device in a cytometric mode provides high throughput and quantitative information about the dimensions and magnetic content of the emulsion. Our method offers important complementarity to conventional optical approaches involving ferrofluids, and paves the way to the development of novel compact tools for diagnostics and nanomedicine including drug design and screening.

Show MeSH