Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat.
Bottom Line: We used a novel noninfectious HIV-1 transgenic (HIV-1Tg) rat model that mimics HIV-1 patients in terms of altered immune responses and deficits in cognitive learning and memory to investigate EtOH concentration-dependent effects on 48 alcohol-modulated genes during binge EtOH administration.Serum blood EtOH concentration (BEC) was measured, and gene expression in the liver and spleen was determined using a specifically designed PCR array.Our data indicate that, in the presence of HIV-1 infection, EtOH concentration-dependent binge drinking can have significantly different molecular effects.
Affiliation: Institute of Neuroimmune Pharmacology, Seton Hall University, South Orange, NJ 07079, USA.Show MeSH
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Mentions: To confirm our observations, we measured the gene expression of 1 representative gene (Gabra1, Grm2, and Drd1a) from each of the 3 neurotransmitter receptor families (GABA, mGlu, and dopamine; Fig. 7). As observed in our PCR array, no significant EtOH concentration-dependent differences were observed in the F344 rats. For all 3 genes (Gabra1 [Fig. 7A], Grm2 [Fig. 7B], and Drd1a [Fig. 7C]), the 8% EtOH group had a higher fold increase (1-fold for Gabra1; 1-fold for Grm2; 2.2-fold for Drd1a) than the 52% EtOH group (−2.2-fold for Gabra1; −2.7-fold for Grm2; 1.5-fold for Drd1a), when compared to control, although the changes were not statistically significant.
Affiliation: Institute of Neuroimmune Pharmacology, Seton Hall University, South Orange, NJ 07079, USA.