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Green tea extract increases mRNA expression of enzymes which influence epigenetic marks in newborn female offspring from undernourished pregnant mother.

Sun Y, Mukai Y, Tanaka M, Saito T, Sato S, Kurasaki M - PLoS ONE (2013)

Bottom Line: We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney.The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys.In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, Japan.

ABSTRACT
Biochemical and toxicological properties of catechin remain unclear, e.g.; how catechin affects female offspring from undernourished pregnant dams. Here, to elucidate effects of low prenatal protein on female offspring health status, changes of enzymes which modify epigenetic marks related with metabolism in kidneys from newborns were investigated after continuously administering catechin extracted from green tea to lactating maternal rats after pregnant undernourishment. We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney. This pathway was indicated to be stimulated by SIRT1 gene expression. The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys. In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

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Protein abundance of eNOS in the kidneys of 3 week old postnatal offspring using western blot analysis.A) phosphorylated eNOS to β-actin, B) eNOS to β-actin and C) phosphorylated eNOS to total eNOS. Values are expressed as means ± S.E.M. (n = 4-7). eNOS-p, phosphorylated eNOS; CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
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pone-0074559-g006: Protein abundance of eNOS in the kidneys of 3 week old postnatal offspring using western blot analysis.A) phosphorylated eNOS to β-actin, B) eNOS to β-actin and C) phosphorylated eNOS to total eNOS. Values are expressed as means ± S.E.M. (n = 4-7). eNOS-p, phosphorylated eNOS; CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.

Mentions: Because AMPK is reported to be associated with the phosphorylation of eNOS, resulting in an increased release of NO [27,28], we investigated the expression and phosphorylation of eNOS. Although phosphorylated eNOS in the of low-protein group with high GTE dose significantly increased when compared with the control group, phosphorylated eNOS among groups was not different in low-protein groups (Figure 6A). As shown in Figure 6B and C, no significant difference was found, respectively, for eNOS and phosphorylated eNOS /eNOS ratio among all groups.


Green tea extract increases mRNA expression of enzymes which influence epigenetic marks in newborn female offspring from undernourished pregnant mother.

Sun Y, Mukai Y, Tanaka M, Saito T, Sato S, Kurasaki M - PLoS ONE (2013)

Protein abundance of eNOS in the kidneys of 3 week old postnatal offspring using western blot analysis.A) phosphorylated eNOS to β-actin, B) eNOS to β-actin and C) phosphorylated eNOS to total eNOS. Values are expressed as means ± S.E.M. (n = 4-7). eNOS-p, phosphorylated eNOS; CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756974&req=5

pone-0074559-g006: Protein abundance of eNOS in the kidneys of 3 week old postnatal offspring using western blot analysis.A) phosphorylated eNOS to β-actin, B) eNOS to β-actin and C) phosphorylated eNOS to total eNOS. Values are expressed as means ± S.E.M. (n = 4-7). eNOS-p, phosphorylated eNOS; CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
Mentions: Because AMPK is reported to be associated with the phosphorylation of eNOS, resulting in an increased release of NO [27,28], we investigated the expression and phosphorylation of eNOS. Although phosphorylated eNOS in the of low-protein group with high GTE dose significantly increased when compared with the control group, phosphorylated eNOS among groups was not different in low-protein groups (Figure 6A). As shown in Figure 6B and C, no significant difference was found, respectively, for eNOS and phosphorylated eNOS /eNOS ratio among all groups.

Bottom Line: We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney.The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys.In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, Japan.

ABSTRACT
Biochemical and toxicological properties of catechin remain unclear, e.g.; how catechin affects female offspring from undernourished pregnant dams. Here, to elucidate effects of low prenatal protein on female offspring health status, changes of enzymes which modify epigenetic marks related with metabolism in kidneys from newborns were investigated after continuously administering catechin extracted from green tea to lactating maternal rats after pregnant undernourishment. We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney. This pathway was indicated to be stimulated by SIRT1 gene expression. The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys. In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

Show MeSH
Related in: MedlinePlus