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Green tea extract increases mRNA expression of enzymes which influence epigenetic marks in newborn female offspring from undernourished pregnant mother.

Sun Y, Mukai Y, Tanaka M, Saito T, Sato S, Kurasaki M - PLoS ONE (2013)

Bottom Line: We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney.The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys.In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, Japan.

ABSTRACT
Biochemical and toxicological properties of catechin remain unclear, e.g.; how catechin affects female offspring from undernourished pregnant dams. Here, to elucidate effects of low prenatal protein on female offspring health status, changes of enzymes which modify epigenetic marks related with metabolism in kidneys from newborns were investigated after continuously administering catechin extracted from green tea to lactating maternal rats after pregnant undernourishment. We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney. This pathway was indicated to be stimulated by SIRT1 gene expression. The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys. In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

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Protein abundance of p53 to β-actin in the kidneys of 3 week old postnatal offspring using western blot analysis.Values are expressed as means ± S.E.M. (n = 4). CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
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pone-0074559-g004: Protein abundance of p53 to β-actin in the kidneys of 3 week old postnatal offspring using western blot analysis.Values are expressed as means ± S.E.M. (n = 4). CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.

Mentions: To test if longevity related gene expression was sensitive to different regimens of maternal nourishment, SIRT1 and 2 were examined in kidneys from offspring of undernourished pregnant rats with or without GTE addition. Figure 3A shows that SIRT1 expression in low-protein group shows a tendency to decrease (p=0.0507) when compared with the control group. By contrast, GTE treatments were similar to the control (for example p=0.54 for control and high GTE treated groups). Similar patterns were observed in the case of SIRT2 (Figure 3B). In detail, p values in SIRT2 gene expression are 0.091 for low protein group and low GTE treated group, and 0.099 for low protein group and high GTE treated group. To investigate changes of downstream of SIRT1, p53 contents were detected in kidneys from offspring of undernourished pregnant rats with or without GTE addition using western blotting (Figure 4). Unexpectedly, p53 was significantly downregulated in the kidney of high dose GTE treated low protein group compared to the un-treated low protein group.


Green tea extract increases mRNA expression of enzymes which influence epigenetic marks in newborn female offspring from undernourished pregnant mother.

Sun Y, Mukai Y, Tanaka M, Saito T, Sato S, Kurasaki M - PLoS ONE (2013)

Protein abundance of p53 to β-actin in the kidneys of 3 week old postnatal offspring using western blot analysis.Values are expressed as means ± S.E.M. (n = 4). CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756974&req=5

pone-0074559-g004: Protein abundance of p53 to β-actin in the kidneys of 3 week old postnatal offspring using western blot analysis.Values are expressed as means ± S.E.M. (n = 4). CC, control on control; LP, control on protein restricted; LPCL, 0.12% GTE diet on protein restricted; LPCH, 0.24% GTE diet on protein restricted. a*P < 0.05 compared with CC. b*P < 0.05 compared with LP.
Mentions: To test if longevity related gene expression was sensitive to different regimens of maternal nourishment, SIRT1 and 2 were examined in kidneys from offspring of undernourished pregnant rats with or without GTE addition. Figure 3A shows that SIRT1 expression in low-protein group shows a tendency to decrease (p=0.0507) when compared with the control group. By contrast, GTE treatments were similar to the control (for example p=0.54 for control and high GTE treated groups). Similar patterns were observed in the case of SIRT2 (Figure 3B). In detail, p values in SIRT2 gene expression are 0.091 for low protein group and low GTE treated group, and 0.099 for low protein group and high GTE treated group. To investigate changes of downstream of SIRT1, p53 contents were detected in kidneys from offspring of undernourished pregnant rats with or without GTE addition using western blotting (Figure 4). Unexpectedly, p53 was significantly downregulated in the kidney of high dose GTE treated low protein group compared to the un-treated low protein group.

Bottom Line: We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney.The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys.In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido, Japan.

ABSTRACT
Biochemical and toxicological properties of catechin remain unclear, e.g.; how catechin affects female offspring from undernourished pregnant dams. Here, to elucidate effects of low prenatal protein on female offspring health status, changes of enzymes which modify epigenetic marks related with metabolism in kidneys from newborns were investigated after continuously administering catechin extracted from green tea to lactating maternal rats after pregnant undernourishment. We found that green tea extract intake during lactation up-regulated the activation of AMP-activated protein kinase in young female offspring from protein-restricted dams and modulated the AMP-activated protein kinase pathway in the kidney. This pathway was indicated to be stimulated by SIRT1 gene expression. The feeding of green tea extract to protein-restricted dams during lactation is likely to up-regulate AMP-activated protein kinase activation and may partly lead to alterations of the AMP-activated protein kinase pathway in female offspring kidneys. In addition, energy metabolism in fetal and offspring period with green tea extract administration might be related to enzymes which modify epigenetic marks such as DNA methyltransferase 1 and 3a.

Show MeSH
Related in: MedlinePlus