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Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.

Cabiati M, Raucci S, Liistro T, Belcastro E, Prescimone T, Caselli C, Matteucci M, Iozzo P, Mattii L, Giannessi D, Del Ry S - PLoS ONE (2013)

Bottom Line: In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO.TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue.The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells.

View Article: PubMed Central - PubMed

Affiliation: CNR Institute of Clinical Physiology, Pisa, Italy.

ABSTRACT
Natriuretic peptides (NPs) play an important role in obesity and aim of this study was to evaluate, in cardiac tissue of obese Zucker rats (O, n = 29) their transcriptomic profile compared to controls (CO, n = 24) by Real-Time PCR study; CNP protein expression was evaluated by immunostaining and immunometric tests. Myocardial histology was performed, confirming no alteration of organ structure. While ANP and BNP are cardiac peptides, CNP is mainly an endothelial hormone; thus its expression, as well as that of NPR-B and NPR-C, was also evaluated in kidney and lung of an animal subgroup (n = 20). In heart, lower BNP mRNA levels in O vs CO (p = 0.02) as well as ANP and CNP (p = ns), were detected. NPR-B/NPR-A mRNA was similar in O and CO, while NPR-C was numerically lower (p = ns) in O than in CO. In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO. Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged. The trend observed in heart, kidney and lung for CNP protein concentrations and immunohistochemistry reflected the mRNA expression. TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue. The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells. The understanding of mechanisms involved in the modulation of the NP system in obesity could be a useful starting point for future clinical study devoted to identifying new obesity treatment strategies.

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CNP, NPR-B and NPR-C mRNA expression in renal and pulmonary tissue of control lean (CO, white bar) and obese Zucker rats (O, black bar).On the left side: a) CNP, b) NPR-B, c) NPR-C relative levels in kidney. On the right side: d) CNP, e) NPR-B, f) NPR-C relative levels in lung.
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pone-0072959-g004: CNP, NPR-B and NPR-C mRNA expression in renal and pulmonary tissue of control lean (CO, white bar) and obese Zucker rats (O, black bar).On the left side: a) CNP, b) NPR-B, c) NPR-C relative levels in kidney. On the right side: d) CNP, e) NPR-B, f) NPR-C relative levels in lung.

Mentions: In the kidney, CNP, NPR-B and NPR-C mRNA expression was similar in both O and CO rats (Fig. 4 a–c), while in the lung CNP and NPR-C transcription levels decreased in parallel with an increased NPR-B expression, although not significantly, in obese Zucker rats with respect to controls (Fig. 4 d–f). Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged.


Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model.

Cabiati M, Raucci S, Liistro T, Belcastro E, Prescimone T, Caselli C, Matteucci M, Iozzo P, Mattii L, Giannessi D, Del Ry S - PLoS ONE (2013)

CNP, NPR-B and NPR-C mRNA expression in renal and pulmonary tissue of control lean (CO, white bar) and obese Zucker rats (O, black bar).On the left side: a) CNP, b) NPR-B, c) NPR-C relative levels in kidney. On the right side: d) CNP, e) NPR-B, f) NPR-C relative levels in lung.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756951&req=5

pone-0072959-g004: CNP, NPR-B and NPR-C mRNA expression in renal and pulmonary tissue of control lean (CO, white bar) and obese Zucker rats (O, black bar).On the left side: a) CNP, b) NPR-B, c) NPR-C relative levels in kidney. On the right side: d) CNP, e) NPR-B, f) NPR-C relative levels in lung.
Mentions: In the kidney, CNP, NPR-B and NPR-C mRNA expression was similar in both O and CO rats (Fig. 4 a–c), while in the lung CNP and NPR-C transcription levels decreased in parallel with an increased NPR-B expression, although not significantly, in obese Zucker rats with respect to controls (Fig. 4 d–f). Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged.

Bottom Line: In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO.TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue.The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells.

View Article: PubMed Central - PubMed

Affiliation: CNR Institute of Clinical Physiology, Pisa, Italy.

ABSTRACT
Natriuretic peptides (NPs) play an important role in obesity and aim of this study was to evaluate, in cardiac tissue of obese Zucker rats (O, n = 29) their transcriptomic profile compared to controls (CO, n = 24) by Real-Time PCR study; CNP protein expression was evaluated by immunostaining and immunometric tests. Myocardial histology was performed, confirming no alteration of organ structure. While ANP and BNP are cardiac peptides, CNP is mainly an endothelial hormone; thus its expression, as well as that of NPR-B and NPR-C, was also evaluated in kidney and lung of an animal subgroup (n = 20). In heart, lower BNP mRNA levels in O vs CO (p = 0.02) as well as ANP and CNP (p = ns), were detected. NPR-B/NPR-A mRNA was similar in O and CO, while NPR-C was numerically lower (p = ns) in O than in CO. In kidney, CNP/NPR-B/NPR-C mRNA was similar in O and CO, while in lung CNP/NPR-C expression decreased and NPR-B increased (p = ns) in O vs CO. Subdividing into fasting and hyperglycemic rats, the pattern of mRNA expression for each gene analyzed remained unchanged. The trend observed in heart, kidney and lung for CNP protein concentrations and immunohistochemistry reflected the mRNA expression. TNF-α and IL-6 mRNA were measured in each tissue and no significant genotype effect was detected in any tissue. The main NP variations were observed at the cardiac level, suggesting a reduced release by cardiac cells. The understanding of mechanisms involved in the modulation of the NP system in obesity could be a useful starting point for future clinical study devoted to identifying new obesity treatment strategies.

Show MeSH
Related in: MedlinePlus