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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.A silica gel 60 plate (Merck, Darmstadt) was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Lane 1, crude lipid from glands of intact males; lane 2, castrated males; lane 3, intact females; lane 4, testosterone-treated females. ADG: 1-alkyl-2,3-diacylglycerol; FFA: free fatty acid.
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f9-83_077: Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.A silica gel 60 plate (Merck, Darmstadt) was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Lane 1, crude lipid from glands of intact males; lane 2, castrated males; lane 3, intact females; lane 4, testosterone-treated females. ADG: 1-alkyl-2,3-diacylglycerol; FFA: free fatty acid.

Mentions: The primary endocrine control of Harderian ADG appeared to be due to androgens. Thin layer chromatograms (Fig. 947)) from lipid extracts of intact male hamsters (lane 1) showed ADGs as an elongated spots. Castrated male hamsters (lane 2) showed two distinct spots, similar to those seen intact female. Intact female hamsters (lane 3) showed two distinct spots, a faint upper spot and a lower intense spot. ADGs of female hamsters supplied with exogenous testosterone (lane 4) showed TLC patterns indistinguishable from those of intact males, with an elongated triplet spot.


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.A silica gel 60 plate (Merck, Darmstadt) was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Lane 1, crude lipid from glands of intact males; lane 2, castrated males; lane 3, intact females; lane 4, testosterone-treated females. ADG: 1-alkyl-2,3-diacylglycerol; FFA: free fatty acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756879&req=5

f9-83_077: Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.A silica gel 60 plate (Merck, Darmstadt) was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Lane 1, crude lipid from glands of intact males; lane 2, castrated males; lane 3, intact females; lane 4, testosterone-treated females. ADG: 1-alkyl-2,3-diacylglycerol; FFA: free fatty acid.
Mentions: The primary endocrine control of Harderian ADG appeared to be due to androgens. Thin layer chromatograms (Fig. 947)) from lipid extracts of intact male hamsters (lane 1) showed ADGs as an elongated spots. Castrated male hamsters (lane 2) showed two distinct spots, similar to those seen intact female. Intact female hamsters (lane 3) showed two distinct spots, a faint upper spot and a lower intense spot. ADGs of female hamsters supplied with exogenous testosterone (lane 4) showed TLC patterns indistinguishable from those of intact males, with an elongated triplet spot.

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus