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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.The Harderian glands of golden hamsters were obtained and they were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature. The solvents were evaporated off under N2 gas. Small portions of the lipids were dissolved in chloroform-methanol (2:1, v/v) and ADGs and their subfractions were prepared as described by Seyama et al. (1995). The lipid fractions were then spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, crude lipid from glands of females (F); lane 2, purified female ADG (F-1); lane 3, purified female ADG (F-2); lane 4, crude lipid from glands of males (M); lane 5, purified male ADG (M-1); lane 6, purified male ADG (M-2); lane 7, purified male ADG (M-3). ADG, 1-alkyl-2,3-diacylglycerol; Chol, cholesterol; Orig, origin.
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f3-83_077: Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.The Harderian glands of golden hamsters were obtained and they were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature. The solvents were evaporated off under N2 gas. Small portions of the lipids were dissolved in chloroform-methanol (2:1, v/v) and ADGs and their subfractions were prepared as described by Seyama et al. (1995). The lipid fractions were then spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, crude lipid from glands of females (F); lane 2, purified female ADG (F-1); lane 3, purified female ADG (F-2); lane 4, crude lipid from glands of males (M); lane 5, purified male ADG (M-1); lane 6, purified male ADG (M-2); lane 7, purified male ADG (M-3). ADG, 1-alkyl-2,3-diacylglycerol; Chol, cholesterol; Orig, origin.

Mentions: The diversity of ADG according to gender was clearly demonstrated on thin layer plates by us as shown in Fig. 3.26) ADG (F, lane 1) from females gave two subfractions, F-1 (lane 2) and F-2 (lane 3), and that from males (M, lane 4) was separated into three subfractions, M-1 (lane 5), M-2 (lane 6), and M-3 (lane 7).


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.The Harderian glands of golden hamsters were obtained and they were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature. The solvents were evaporated off under N2 gas. Small portions of the lipids were dissolved in chloroform-methanol (2:1, v/v) and ADGs and their subfractions were prepared as described by Seyama et al. (1995). The lipid fractions were then spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, crude lipid from glands of females (F); lane 2, purified female ADG (F-1); lane 3, purified female ADG (F-2); lane 4, crude lipid from glands of males (M); lane 5, purified male ADG (M-1); lane 6, purified male ADG (M-2); lane 7, purified male ADG (M-3). ADG, 1-alkyl-2,3-diacylglycerol; Chol, cholesterol; Orig, origin.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3756879&req=5

f3-83_077: Thin-layer chromatogram of lipid in Harderian glands of golden hamsters.The Harderian glands of golden hamsters were obtained and they were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature. The solvents were evaporated off under N2 gas. Small portions of the lipids were dissolved in chloroform-methanol (2:1, v/v) and ADGs and their subfractions were prepared as described by Seyama et al. (1995). The lipid fractions were then spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The spots were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, crude lipid from glands of females (F); lane 2, purified female ADG (F-1); lane 3, purified female ADG (F-2); lane 4, crude lipid from glands of males (M); lane 5, purified male ADG (M-1); lane 6, purified male ADG (M-2); lane 7, purified male ADG (M-3). ADG, 1-alkyl-2,3-diacylglycerol; Chol, cholesterol; Orig, origin.
Mentions: The diversity of ADG according to gender was clearly demonstrated on thin layer plates by us as shown in Fig. 3.26) ADG (F, lane 1) from females gave two subfractions, F-1 (lane 2) and F-2 (lane 3), and that from males (M, lane 4) was separated into three subfractions, M-1 (lane 5), M-2 (lane 6), and M-3 (lane 7).

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus