Limits...
Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Structures of 1-alkyl-2,3-diacylglycerol (ADG) and fatty acids in the golden hamster Harderian gland.A, R1- is an alkyl residue; R2CO- and R3CO- are acyl residues. B, Fatty acids form ester linkages at the number 2 and 3 positions of glycerol, to make up the acyl portion of ADG. Fatty alcohols, formed from the corresponding fatty acid, form ether linkages at the number 1 position of glycerol, to make up the alkyl portion of ADG. Methyl branching residues at the (n-1) position in iso-branched chain fatty acids and the (n-2) position in anteiso-branched chain fatty acids.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3756879&req=5

f2-83_077: Structures of 1-alkyl-2,3-diacylglycerol (ADG) and fatty acids in the golden hamster Harderian gland.A, R1- is an alkyl residue; R2CO- and R3CO- are acyl residues. B, Fatty acids form ester linkages at the number 2 and 3 positions of glycerol, to make up the acyl portion of ADG. Fatty alcohols, formed from the corresponding fatty acid, form ether linkages at the number 1 position of glycerol, to make up the alkyl portion of ADG. Methyl branching residues at the (n-1) position in iso-branched chain fatty acids and the (n-2) position in anteiso-branched chain fatty acids.

Mentions: The total lipids in the Harderian gland accounted for about 20% of the fresh tissue. When the lipid components of the hamster Harderian gland were studied by Lin and Nadakavukaren,11) thin layer chromatography (TLC) findings suggested the presence of 1-alkyl-2,3-diacylglycerol (ADG) (Fig. 2). About 92% of the total lipid consisted of ADG, trace amounts of free cholesterol and phospholipids being also present.


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Structures of 1-alkyl-2,3-diacylglycerol (ADG) and fatty acids in the golden hamster Harderian gland.A, R1- is an alkyl residue; R2CO- and R3CO- are acyl residues. B, Fatty acids form ester linkages at the number 2 and 3 positions of glycerol, to make up the acyl portion of ADG. Fatty alcohols, formed from the corresponding fatty acid, form ether linkages at the number 1 position of glycerol, to make up the alkyl portion of ADG. Methyl branching residues at the (n-1) position in iso-branched chain fatty acids and the (n-2) position in anteiso-branched chain fatty acids.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756879&req=5

f2-83_077: Structures of 1-alkyl-2,3-diacylglycerol (ADG) and fatty acids in the golden hamster Harderian gland.A, R1- is an alkyl residue; R2CO- and R3CO- are acyl residues. B, Fatty acids form ester linkages at the number 2 and 3 positions of glycerol, to make up the acyl portion of ADG. Fatty alcohols, formed from the corresponding fatty acid, form ether linkages at the number 1 position of glycerol, to make up the alkyl portion of ADG. Methyl branching residues at the (n-1) position in iso-branched chain fatty acids and the (n-2) position in anteiso-branched chain fatty acids.
Mentions: The total lipids in the Harderian gland accounted for about 20% of the fresh tissue. When the lipid components of the hamster Harderian gland were studied by Lin and Nadakavukaren,11) thin layer chromatography (TLC) findings suggested the presence of 1-alkyl-2,3-diacylglycerol (ADG) (Fig. 2). About 92% of the total lipid consisted of ADG, trace amounts of free cholesterol and phospholipids being also present.

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus