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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Effect of androgen on the expression of IVD and androgen receptor (AR) in the Harderian gland of golden hamsters. Golden hamsters (8 weeks old) were injected subcutaneously with 5 mg/kg of testosterone enanthate and Harderian glands were obtained after indicated time. Total RNA was extracted from them and mRNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for IVD, androgen receptor (AR), and beta-actin. Representative ethidium bromide-stained gels are shown. Castration of male hamsters was done 2 weeks before injection. Lane 1, male; lanes 2 to 4, castrated male; lane 2, 0 day; lane 3, 3 days, lane 4, 7 days after injection.
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f17-83_077: Effect of androgen on the expression of IVD and androgen receptor (AR) in the Harderian gland of golden hamsters. Golden hamsters (8 weeks old) were injected subcutaneously with 5 mg/kg of testosterone enanthate and Harderian glands were obtained after indicated time. Total RNA was extracted from them and mRNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for IVD, androgen receptor (AR), and beta-actin. Representative ethidium bromide-stained gels are shown. Castration of male hamsters was done 2 weeks before injection. Lane 1, male; lanes 2 to 4, castrated male; lane 2, 0 day; lane 3, 3 days, lane 4, 7 days after injection.

Mentions: Total RNA was prepared from male hamster Harderian glands and used for reverse transcriptase-polymerase chain reaction (RT-PCR) using oligonucleotide containing partial sequence of golden hamster cDNA.60) The amplified cDNA was ligated into plasmids and the inserted sequence was determined. Northern blotting showed that IVD mRNA was detected in male gland (lanes 1 and 2), but was not in female (lanes 7 and 8) or castrated male gland (lane 3 and 4) (Fig. 15). Treatment of androgen (both testosterone and testosterone enanthate) induced IVD mRNA expression greatly in female (lanes 9 and 10) and castrated male gland (lanes 5 and 6) (Fig. 15). Tissue distribution of IVD mRNA was also analyzed and only Harderian gland showed a marked sexual difference (lane 1, male; lane 7, female) (Fig. 16). From RT-PCR analysis, it was suggested that the expression of IVD and AR mRNA was induced by androgen as soon as 3 days after TE injection (Fig. 17).


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Effect of androgen on the expression of IVD and androgen receptor (AR) in the Harderian gland of golden hamsters. Golden hamsters (8 weeks old) were injected subcutaneously with 5 mg/kg of testosterone enanthate and Harderian glands were obtained after indicated time. Total RNA was extracted from them and mRNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for IVD, androgen receptor (AR), and beta-actin. Representative ethidium bromide-stained gels are shown. Castration of male hamsters was done 2 weeks before injection. Lane 1, male; lanes 2 to 4, castrated male; lane 2, 0 day; lane 3, 3 days, lane 4, 7 days after injection.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756879&req=5

f17-83_077: Effect of androgen on the expression of IVD and androgen receptor (AR) in the Harderian gland of golden hamsters. Golden hamsters (8 weeks old) were injected subcutaneously with 5 mg/kg of testosterone enanthate and Harderian glands were obtained after indicated time. Total RNA was extracted from them and mRNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for IVD, androgen receptor (AR), and beta-actin. Representative ethidium bromide-stained gels are shown. Castration of male hamsters was done 2 weeks before injection. Lane 1, male; lanes 2 to 4, castrated male; lane 2, 0 day; lane 3, 3 days, lane 4, 7 days after injection.
Mentions: Total RNA was prepared from male hamster Harderian glands and used for reverse transcriptase-polymerase chain reaction (RT-PCR) using oligonucleotide containing partial sequence of golden hamster cDNA.60) The amplified cDNA was ligated into plasmids and the inserted sequence was determined. Northern blotting showed that IVD mRNA was detected in male gland (lanes 1 and 2), but was not in female (lanes 7 and 8) or castrated male gland (lane 3 and 4) (Fig. 15). Treatment of androgen (both testosterone and testosterone enanthate) induced IVD mRNA expression greatly in female (lanes 9 and 10) and castrated male gland (lanes 5 and 6) (Fig. 15). Tissue distribution of IVD mRNA was also analyzed and only Harderian gland showed a marked sexual difference (lane 1, male; lane 7, female) (Fig. 16). From RT-PCR analysis, it was suggested that the expression of IVD and AR mRNA was induced by androgen as soon as 3 days after TE injection (Fig. 17).

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus