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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Thin-layer chromatogram of secreted fluid from the Harderian glands of golden hamsters.Golden hamsters (8 weeks old) were subcutaneously injected with 1 mg/kg of testosterone daily. After 1 week they were anesthetized with pentobarbital and irritated with diethyl ether. They immediately excreted fluids from the Harderian gland onto the eyelid and about 1 μl of them were collected using a 5 μl glass capillary (Drummond). The fluid was directly spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The lipids in the fluid were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, intact male; lane 2, castrated male; lane 3, castrated male treated with testosterone; lane 4, intact female; lane 5, female treated with testosterone. ADG: 1-alkyl-2,3-diacylglycerol; Chol: cholesterol; Orig: origin.
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f13-83_077: Thin-layer chromatogram of secreted fluid from the Harderian glands of golden hamsters.Golden hamsters (8 weeks old) were subcutaneously injected with 1 mg/kg of testosterone daily. After 1 week they were anesthetized with pentobarbital and irritated with diethyl ether. They immediately excreted fluids from the Harderian gland onto the eyelid and about 1 μl of them were collected using a 5 μl glass capillary (Drummond). The fluid was directly spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The lipids in the fluid were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, intact male; lane 2, castrated male; lane 3, castrated male treated with testosterone; lane 4, intact female; lane 5, female treated with testosterone. ADG: 1-alkyl-2,3-diacylglycerol; Chol: cholesterol; Orig: origin.

Mentions: We developed a unique bioassay system of androgen receptor activity in the Harderian gland.58) This gland excretes a large amount of lipids through an only one orifice. The fatty acid composition of the exudates reflects the activity of androgen receptor in the gland. We take an aliquot of lipid with glass capillary under a light anesthesia by ether, and plot it directly on thin layer plate (Fig. 13). This method is a noninvasive monitoring system for the assay of androgen effect. It is easy to operate and sensitive enough.


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Thin-layer chromatogram of secreted fluid from the Harderian glands of golden hamsters.Golden hamsters (8 weeks old) were subcutaneously injected with 1 mg/kg of testosterone daily. After 1 week they were anesthetized with pentobarbital and irritated with diethyl ether. They immediately excreted fluids from the Harderian gland onto the eyelid and about 1 μl of them were collected using a 5 μl glass capillary (Drummond). The fluid was directly spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The lipids in the fluid were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, intact male; lane 2, castrated male; lane 3, castrated male treated with testosterone; lane 4, intact female; lane 5, female treated with testosterone. ADG: 1-alkyl-2,3-diacylglycerol; Chol: cholesterol; Orig: origin.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3756879&req=5

f13-83_077: Thin-layer chromatogram of secreted fluid from the Harderian glands of golden hamsters.Golden hamsters (8 weeks old) were subcutaneously injected with 1 mg/kg of testosterone daily. After 1 week they were anesthetized with pentobarbital and irritated with diethyl ether. They immediately excreted fluids from the Harderian gland onto the eyelid and about 1 μl of them were collected using a 5 μl glass capillary (Drummond). The fluid was directly spotted on a silica gel 60 plate (Merck, Darmstadt) and the plate was developed with hexane-diethyl ether-acetic acid (80:20:1, v/v/v). The lipids in the fluid were located by heating the plate at 120 °C after spraying it with 20% sulfuric acid. Samples: lane 1, intact male; lane 2, castrated male; lane 3, castrated male treated with testosterone; lane 4, intact female; lane 5, female treated with testosterone. ADG: 1-alkyl-2,3-diacylglycerol; Chol: cholesterol; Orig: origin.
Mentions: We developed a unique bioassay system of androgen receptor activity in the Harderian gland.58) This gland excretes a large amount of lipids through an only one orifice. The fatty acid composition of the exudates reflects the activity of androgen receptor in the gland. We take an aliquot of lipid with glass capillary under a light anesthesia by ether, and plot it directly on thin layer plate (Fig. 13). This method is a noninvasive monitoring system for the assay of androgen effect. It is easy to operate and sensitive enough.

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus