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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

Thin-layer chromatogram of lipids in the Harderian glands of golden hamsters.Golden hamsters were injected with 2.5 μCi of [U-14C]leucine into peritoneum. Lipids in the gland were obtained and developed on a TLC plate. A, a representative autoradiogram of a plate. B, H2SO4 detection of lipids on the same plate in panel A. Lipids obtained at 1 h after injection were spotted on lanes 1 and 4, at 3 h on lanes 2 and 5, and at 6 h on lanes 3 and 6. Lanes 1 to 3 contained male lipids, while lanes 4 to 6 contained female lipids. “ADG” and “FFA” indicate the positions of 1-alkyl-2,3-diacylglycerol and free fatty acids, respectively.
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f11-83_077: Thin-layer chromatogram of lipids in the Harderian glands of golden hamsters.Golden hamsters were injected with 2.5 μCi of [U-14C]leucine into peritoneum. Lipids in the gland were obtained and developed on a TLC plate. A, a representative autoradiogram of a plate. B, H2SO4 detection of lipids on the same plate in panel A. Lipids obtained at 1 h after injection were spotted on lanes 1 and 4, at 3 h on lanes 2 and 5, and at 6 h on lanes 3 and 6. Lanes 1 to 3 contained male lipids, while lanes 4 to 6 contained female lipids. “ADG” and “FFA” indicate the positions of 1-alkyl-2,3-diacylglycerol and free fatty acids, respectively.

Mentions: Lipid extracts were obtained from glands at 1, 3, and 6 h after injection, developed on TLC plates, and then analyzed by autoradiography. Fig. 1145) shows a representative autoradiogram when [U-14C] leucine was injected. The most strongly labeled fraction was ADG both in male and females, and the incorporation of the isotope into the ADG fraction reached the maximal level at 6 h after injection. But the radioactivity incorporation profiles (A) differed with the gender, like those observed of the total ADG detected by H2SO4 (B). The cholesterol fraction was labeled in males, but not in females, although a total cholesterol spot was detected in both sexes.


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Thin-layer chromatogram of lipids in the Harderian glands of golden hamsters.Golden hamsters were injected with 2.5 μCi of [U-14C]leucine into peritoneum. Lipids in the gland were obtained and developed on a TLC plate. A, a representative autoradiogram of a plate. B, H2SO4 detection of lipids on the same plate in panel A. Lipids obtained at 1 h after injection were spotted on lanes 1 and 4, at 3 h on lanes 2 and 5, and at 6 h on lanes 3 and 6. Lanes 1 to 3 contained male lipids, while lanes 4 to 6 contained female lipids. “ADG” and “FFA” indicate the positions of 1-alkyl-2,3-diacylglycerol and free fatty acids, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756879&req=5

f11-83_077: Thin-layer chromatogram of lipids in the Harderian glands of golden hamsters.Golden hamsters were injected with 2.5 μCi of [U-14C]leucine into peritoneum. Lipids in the gland were obtained and developed on a TLC plate. A, a representative autoradiogram of a plate. B, H2SO4 detection of lipids on the same plate in panel A. Lipids obtained at 1 h after injection were spotted on lanes 1 and 4, at 3 h on lanes 2 and 5, and at 6 h on lanes 3 and 6. Lanes 1 to 3 contained male lipids, while lanes 4 to 6 contained female lipids. “ADG” and “FFA” indicate the positions of 1-alkyl-2,3-diacylglycerol and free fatty acids, respectively.
Mentions: Lipid extracts were obtained from glands at 1, 3, and 6 h after injection, developed on TLC plates, and then analyzed by autoradiography. Fig. 1145) shows a representative autoradiogram when [U-14C] leucine was injected. The most strongly labeled fraction was ADG both in male and females, and the incorporation of the isotope into the ADG fraction reached the maximal level at 6 h after injection. But the radioactivity incorporation profiles (A) differed with the gender, like those observed of the total ADG detected by H2SO4 (B). The cholesterol fraction was labeled in males, but not in females, although a total cholesterol spot was detected in both sexes.

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus