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Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus

The incorporation of radioactivity into lipids in tissues of golden hamsters after [U-14C]leucine injection.Golden hamsters were injected intraperitoneally with 2.5 μCi of [U-14C]leucine (Du Pont, Boston, MA, USA) diluted with Dulbecco’s phosphate-buffered saline (D-PBS). Three, 6, 9 and 24 h after injection, Harderian gland, liver, brain, and heart were obtained under anesthesia with sodium pentobarbital. They were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature and then lipids were extracted in the liquid phase. Five hundred micro liters of this fraction was evaporated under N2 gas, and then dissolved in 2 ml of Clear-sol I (Nacalai Tesque, Kyoto). Radioactivity in the solution was measured with a liquid scintillation spectrometer (Tri-Carb 15000; Packard Instruments, Downers Grove, IL, USA).
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f10-83_077: The incorporation of radioactivity into lipids in tissues of golden hamsters after [U-14C]leucine injection.Golden hamsters were injected intraperitoneally with 2.5 μCi of [U-14C]leucine (Du Pont, Boston, MA, USA) diluted with Dulbecco’s phosphate-buffered saline (D-PBS). Three, 6, 9 and 24 h after injection, Harderian gland, liver, brain, and heart were obtained under anesthesia with sodium pentobarbital. They were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature and then lipids were extracted in the liquid phase. Five hundred micro liters of this fraction was evaporated under N2 gas, and then dissolved in 2 ml of Clear-sol I (Nacalai Tesque, Kyoto). Radioactivity in the solution was measured with a liquid scintillation spectrometer (Tri-Carb 15000; Packard Instruments, Downers Grove, IL, USA).

Mentions: The radioactivity incorporated into lipids was monitored after the incorporation of [U-14C] leucine into peritoneum of golden hamsters (Fig. 1045)). The leucine metabolites were efficiently incorporated into the lipids in the Harderian gland in both sexes as compared with those in other tissues such as liver, heart, and brain. The level of tracer incorporation reached the maximum after 6 to 9 h and then decreased gradually. Similar results were obtained when [U-14C] isoleucine or [U-14C] valine was injected.


Novel function of lipids as a pheromone from the Harderian gland of golden hamster.

Seyama Y, Uchijima Y - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

The incorporation of radioactivity into lipids in tissues of golden hamsters after [U-14C]leucine injection.Golden hamsters were injected intraperitoneally with 2.5 μCi of [U-14C]leucine (Du Pont, Boston, MA, USA) diluted with Dulbecco’s phosphate-buffered saline (D-PBS). Three, 6, 9 and 24 h after injection, Harderian gland, liver, brain, and heart were obtained under anesthesia with sodium pentobarbital. They were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature and then lipids were extracted in the liquid phase. Five hundred micro liters of this fraction was evaporated under N2 gas, and then dissolved in 2 ml of Clear-sol I (Nacalai Tesque, Kyoto). Radioactivity in the solution was measured with a liquid scintillation spectrometer (Tri-Carb 15000; Packard Instruments, Downers Grove, IL, USA).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756879&req=5

f10-83_077: The incorporation of radioactivity into lipids in tissues of golden hamsters after [U-14C]leucine injection.Golden hamsters were injected intraperitoneally with 2.5 μCi of [U-14C]leucine (Du Pont, Boston, MA, USA) diluted with Dulbecco’s phosphate-buffered saline (D-PBS). Three, 6, 9 and 24 h after injection, Harderian gland, liver, brain, and heart were obtained under anesthesia with sodium pentobarbital. They were kept overnight in 5 ml of chloroform/methanol (2:1, v/v) at room temperature and then lipids were extracted in the liquid phase. Five hundred micro liters of this fraction was evaporated under N2 gas, and then dissolved in 2 ml of Clear-sol I (Nacalai Tesque, Kyoto). Radioactivity in the solution was measured with a liquid scintillation spectrometer (Tri-Carb 15000; Packard Instruments, Downers Grove, IL, USA).
Mentions: The radioactivity incorporated into lipids was monitored after the incorporation of [U-14C] leucine into peritoneum of golden hamsters (Fig. 1045)). The leucine metabolites were efficiently incorporated into the lipids in the Harderian gland in both sexes as compared with those in other tissues such as liver, heart, and brain. The level of tracer incorporation reached the maximum after 6 to 9 h and then decreased gradually. Similar results were obtained when [U-14C] isoleucine or [U-14C] valine was injected.

Bottom Line: The incorporation of [U-(14)C] leucine into lipids was monitored by TLC.The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases.We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant.

View Article: PubMed Central - PubMed

Affiliation: Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .

ABSTRACT
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.

No MeSH data available.


Related in: MedlinePlus