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Phenotypic reversion in analbuminemic rats due to an altered splicing mechanism.

Esumi H, Sugimura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters.Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping.The altered albumin molecules thus synthesized accumulated in cellular organelles.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba, Japan .

ABSTRACT
Serum albumin is regarded as an important and indispensable protein, but analbuminemic rats established by Sumi Nagase in 1977 seems to exhibit few symptoms in spite of an almost total lack of albumin in the serum. The albumin gene of analbuminemic rats was found to have a seven-base-pair deletion in an intron, close to exon-intron junction, resulting in the formation of non-functional mRNA in hepatocytes. Immunostaining for albumin was negative in young analbuminemic rat hepatocytes, but a significant number of immunoreactive hepatocytes were observed in aged rats. The incidence of immunoreactive hepatocytes increased with aging. Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters. Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping. The altered albumin molecules thus synthesized accumulated in cellular organelles. Analbuminemic rats exhibited a high sensitivity in various organs to different types of carcinogens. Further challenges remain regarding the biology of analbuminemic rats.

No MeSH data available.


The statue of Dr. Takaoki Sasaki at the Sasaki Institute and picture of old building of the Sasaki Institute.
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f8-83_101: The statue of Dr. Takaoki Sasaki at the Sasaki Institute and picture of old building of the Sasaki Institute.

Mentions: We are very much in debt to Dr. Sumi Nagase of the Sasaki Institute for our work on analbuminemic rats. The Sasaki Institute (Fig. 8) was established in 1939 by Dr. Takaoki Sasaki, who was an eminent scientist and oncologist, and in addition a pioneer in research on amino acid metabolism. Dr. Sasaki is rightly famous for having established the hepatocarcinogenicity of azo-dyes in collaboration with Dr. Tomizo Yoshida in his laboratories. The contributions of Sasaki Institute, a privately owned foundation, deserve our deepest appreciation.Fig. 8


Phenotypic reversion in analbuminemic rats due to an altered splicing mechanism.

Esumi H, Sugimura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

The statue of Dr. Takaoki Sasaki at the Sasaki Institute and picture of old building of the Sasaki Institute.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756734&req=5

f8-83_101: The statue of Dr. Takaoki Sasaki at the Sasaki Institute and picture of old building of the Sasaki Institute.
Mentions: We are very much in debt to Dr. Sumi Nagase of the Sasaki Institute for our work on analbuminemic rats. The Sasaki Institute (Fig. 8) was established in 1939 by Dr. Takaoki Sasaki, who was an eminent scientist and oncologist, and in addition a pioneer in research on amino acid metabolism. Dr. Sasaki is rightly famous for having established the hepatocarcinogenicity of azo-dyes in collaboration with Dr. Tomizo Yoshida in his laboratories. The contributions of Sasaki Institute, a privately owned foundation, deserve our deepest appreciation.Fig. 8

Bottom Line: Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters.Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping.The altered albumin molecules thus synthesized accumulated in cellular organelles.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba, Japan .

ABSTRACT
Serum albumin is regarded as an important and indispensable protein, but analbuminemic rats established by Sumi Nagase in 1977 seems to exhibit few symptoms in spite of an almost total lack of albumin in the serum. The albumin gene of analbuminemic rats was found to have a seven-base-pair deletion in an intron, close to exon-intron junction, resulting in the formation of non-functional mRNA in hepatocytes. Immunostaining for albumin was negative in young analbuminemic rat hepatocytes, but a significant number of immunoreactive hepatocytes were observed in aged rats. The incidence of immunoreactive hepatocytes increased with aging. Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters. Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping. The altered albumin molecules thus synthesized accumulated in cellular organelles. Analbuminemic rats exhibited a high sensitivity in various organs to different types of carcinogens. Further challenges remain regarding the biology of analbuminemic rats.

No MeSH data available.