Limits...
Phenotypic reversion in analbuminemic rats due to an altered splicing mechanism.

Esumi H, Sugimura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Bottom Line: Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters.Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping.The altered albumin molecules thus synthesized accumulated in cellular organelles.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba, Japan .

ABSTRACT
Serum albumin is regarded as an important and indispensable protein, but analbuminemic rats established by Sumi Nagase in 1977 seems to exhibit few symptoms in spite of an almost total lack of albumin in the serum. The albumin gene of analbuminemic rats was found to have a seven-base-pair deletion in an intron, close to exon-intron junction, resulting in the formation of non-functional mRNA in hepatocytes. Immunostaining for albumin was negative in young analbuminemic rat hepatocytes, but a significant number of immunoreactive hepatocytes were observed in aged rats. The incidence of immunoreactive hepatocytes increased with aging. Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters. Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping. The altered albumin molecules thus synthesized accumulated in cellular organelles. Analbuminemic rats exhibited a high sensitivity in various organs to different types of carcinogens. Further challenges remain regarding the biology of analbuminemic rats.

No MeSH data available.


Polyacrylamide gel electrophoregrams of serum from a Sprague-Dawley rat and an analbuminemic rat.The serum proteins were separated on 5% polyacrylamide and stained with Coomassie Brilliant Blue. The arrow indicates the albumin band (Adopted with permission from Biochem. Biophys. Res. Commun. 87, 1191–1199 (1979)).21)
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3756734&req=5

f1-83_101: Polyacrylamide gel electrophoregrams of serum from a Sprague-Dawley rat and an analbuminemic rat.The serum proteins were separated on 5% polyacrylamide and stained with Coomassie Brilliant Blue. The arrow indicates the albumin band (Adopted with permission from Biochem. Biophys. Res. Commun. 87, 1191–1199 (1979)).21)

Mentions: We had the good fortune to learn of this unique rat strain from Nagase, who telephoned one of the authors (TS) to convey the exciting news about her discovery of the mutant rat. She complained that nobody believed her surprising discovery but Nagase brought an electrophoregram of the serum proteins of an analbuminemic rat to our laboratory and, as shown in Fig. 1, the absolute lack of albumin band was clear. We therefore launched a collaborative study to elucidate the underlying molecular mechanisms.


Phenotypic reversion in analbuminemic rats due to an altered splicing mechanism.

Esumi H, Sugimura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2007)

Polyacrylamide gel electrophoregrams of serum from a Sprague-Dawley rat and an analbuminemic rat.The serum proteins were separated on 5% polyacrylamide and stained with Coomassie Brilliant Blue. The arrow indicates the albumin band (Adopted with permission from Biochem. Biophys. Res. Commun. 87, 1191–1199 (1979)).21)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756734&req=5

f1-83_101: Polyacrylamide gel electrophoregrams of serum from a Sprague-Dawley rat and an analbuminemic rat.The serum proteins were separated on 5% polyacrylamide and stained with Coomassie Brilliant Blue. The arrow indicates the albumin band (Adopted with permission from Biochem. Biophys. Res. Commun. 87, 1191–1199 (1979)).21)
Mentions: We had the good fortune to learn of this unique rat strain from Nagase, who telephoned one of the authors (TS) to convey the exciting news about her discovery of the mutant rat. She complained that nobody believed her surprising discovery but Nagase brought an electrophoregram of the serum proteins of an analbuminemic rat to our laboratory and, as shown in Fig. 1, the absolute lack of albumin band was clear. We therefore launched a collaborative study to elucidate the underlying molecular mechanisms.

Bottom Line: Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters.Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping.The altered albumin molecules thus synthesized accumulated in cellular organelles.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Innovative Oncology, National Cancer Center Hospital East, Chiba, Japan .

ABSTRACT
Serum albumin is regarded as an important and indispensable protein, but analbuminemic rats established by Sumi Nagase in 1977 seems to exhibit few symptoms in spite of an almost total lack of albumin in the serum. The albumin gene of analbuminemic rats was found to have a seven-base-pair deletion in an intron, close to exon-intron junction, resulting in the formation of non-functional mRNA in hepatocytes. Immunostaining for albumin was negative in young analbuminemic rat hepatocytes, but a significant number of immunoreactive hepatocytes were observed in aged rats. The incidence of immunoreactive hepatocytes increased with aging. Surprisingly, many immunoreactive hepatocytes were observed after hepatocarcinogen treatment sometimes in large clusters. Albumin transcripts in analbuminemic rat liver after treatment with carcinogen, showed an altered pattern of exon-skipping. The altered albumin molecules thus synthesized accumulated in cellular organelles. Analbuminemic rats exhibited a high sensitivity in various organs to different types of carcinogens. Further challenges remain regarding the biology of analbuminemic rats.

No MeSH data available.