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A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

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Calibration curve of H2O2 biosensor with HRP immobilized by (a) PPy film and (b) physical adsorption. The inset indicates calibration curve obtained for the linear range. Experimental condition: 1 µL H2O2, -300 mV applied potential.
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f8-sensors-07-00239: Calibration curve of H2O2 biosensor with HRP immobilized by (a) PPy film and (b) physical adsorption. The inset indicates calibration curve obtained for the linear range. Experimental condition: 1 µL H2O2, -300 mV applied potential.

Mentions: The calibration curve of the sensor is shown as curve (a) in Figure 8. Eight hydrogen peroxide samples of different concentrations were measured using the biosensor fabricated under the optimized condition as described, and each sample of a certain concentration was measured five times. The inset illustration indicates the linear range of the PPy-HRP electrode response was from 0.1 to 2 mM, and the sensitivity corresponding to the linear range was about 33.24 µA mM-1 cm-2. The equation of the response was [Y(µA)=0.6647X(mM)-0.0234, R2=0.9953].


A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes
Calibration curve of H2O2 biosensor with HRP immobilized by (a) PPy film and (b) physical adsorption. The inset indicates calibration curve obtained for the linear range. Experimental condition: 1 µL H2O2, -300 mV applied potential.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756719&req=5

f8-sensors-07-00239: Calibration curve of H2O2 biosensor with HRP immobilized by (a) PPy film and (b) physical adsorption. The inset indicates calibration curve obtained for the linear range. Experimental condition: 1 µL H2O2, -300 mV applied potential.
Mentions: The calibration curve of the sensor is shown as curve (a) in Figure 8. Eight hydrogen peroxide samples of different concentrations were measured using the biosensor fabricated under the optimized condition as described, and each sample of a certain concentration was measured five times. The inset illustration indicates the linear range of the PPy-HRP electrode response was from 0.1 to 2 mM, and the sensitivity corresponding to the linear range was about 33.24 µA mM-1 cm-2. The equation of the response was [Y(µA)=0.6647X(mM)-0.0234, R2=0.9953].

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

No MeSH data available.


Related in: MedlinePlus