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A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

No MeSH data available.


Effect of HRP concentration on response current to 1 mM H2O2 (1 µL, -300 mV potential applied).
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f5-sensors-07-00239: Effect of HRP concentration on response current to 1 mM H2O2 (1 µL, -300 mV potential applied).

Mentions: The HRP concentration in the pyrrole monomer solution for preparing the enzyme electrode was a key factor to affect the sensitivity, because the sensor response depended upon the amount of immobilized HRP. Figure 5 shows the response current to 1 mM H2O2 of the sensors using HRP of different concentration. With the increase of HRP concentration from 0.2mg/ml to 0.8mg/ml, the response current was almost doubled. When the HRP concentration was higher than 0.8 mg/ml, the response current of the sensor did not increase further. So the HRP concentration of 0.8mg/ml was used in the experiments for the economic consideration.


A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes
Effect of HRP concentration on response current to 1 mM H2O2 (1 µL, -300 mV potential applied).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756719&req=5

f5-sensors-07-00239: Effect of HRP concentration on response current to 1 mM H2O2 (1 µL, -300 mV potential applied).
Mentions: The HRP concentration in the pyrrole monomer solution for preparing the enzyme electrode was a key factor to affect the sensitivity, because the sensor response depended upon the amount of immobilized HRP. Figure 5 shows the response current to 1 mM H2O2 of the sensors using HRP of different concentration. With the increase of HRP concentration from 0.2mg/ml to 0.8mg/ml, the response current was almost doubled. When the HRP concentration was higher than 0.8 mg/ml, the response current of the sensor did not increase further. So the HRP concentration of 0.8mg/ml was used in the experiments for the economic consideration.

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

No MeSH data available.