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A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

No MeSH data available.


The structure of the screen-printed electrodes, the sensor substrate was 35 mm×10 mm while the working electrode area was 1 mm×2 mm.
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f1-sensors-07-00239: The structure of the screen-printed electrodes, the sensor substrate was 35 mm×10 mm while the working electrode area was 1 mm×2 mm.

Mentions: Screen-printed electrodes were fabricated on polypropylene (PP) sheets using the screen-printer. Three masks were used to form sliver conducting lead wires, carbon paste film for electrochemical reaction and insulating film, the process has been described in detail previously [22]. The structure of the biosensor is shown in Figure 1, consisting of an Ag/AgCl pseudo-reference electrode and two carbon electrodes, which acting as working and counter electrodes, respectively. The function of the silver leads was to improve electric conductivity of the electrodes. The reaction area was defined by the insulating film covering on the carbon paste film. The size of working electrode in reaction area was 1 mm×2 mm while the sensor substrate was 35 mm×10 mm.


A Hydrogen Peroxide Sensor Prepared by Electropolymerization of Pyrrole Based on Screen-Printed Carbon Paste Electrodes
The structure of the screen-printed electrodes, the sensor substrate was 35 mm×10 mm while the working electrode area was 1 mm×2 mm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756719&req=5

f1-sensors-07-00239: The structure of the screen-printed electrodes, the sensor substrate was 35 mm×10 mm while the working electrode area was 1 mm×2 mm.
Mentions: Screen-printed electrodes were fabricated on polypropylene (PP) sheets using the screen-printer. Three masks were used to form sliver conducting lead wires, carbon paste film for electrochemical reaction and insulating film, the process has been described in detail previously [22]. The structure of the biosensor is shown in Figure 1, consisting of an Ag/AgCl pseudo-reference electrode and two carbon electrodes, which acting as working and counter electrodes, respectively. The function of the silver leads was to improve electric conductivity of the electrodes. The reaction area was defined by the insulating film covering on the carbon paste film. The size of working electrode in reaction area was 1 mm×2 mm while the sensor substrate was 35 mm×10 mm.

View Article: PubMed Central

ABSTRACT

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.

No MeSH data available.