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Influence of Different Genotypes on Trypsin Inhibitor Levels and Activity in Soybeans

View Article: PubMed Central

ABSTRACT

This study describes the relationship between the two major trypsin inhibitors (TI) in soybean, i.e., the Kunitz (KTI) and Bowman-Birk (BBI) trypsin inhibitors, as well as between them and the corresponding trypsin inhibitor activity (TIA). Twelve investigated soybean genotypes showed significant differences in TI levels and TIA. A very strong positive correlation was found between the levels of KTI and total BBI (r = 0.94, P < 0.05). No relationship was found between KTI, BBI or total TI and TIA. Based on this data, it appears that the levels of major TI in soybean are related. Understanding the relationship between trypsin inhibitors and their activities could be useful for further improvement of the health impacts of soy proteins.

No MeSH data available.


PAGE analysis of the protein extracts. Lanes 1-12 represent the electrophoretic patterns of the Proteinka, Balkan, Ravnica, Vojvodjanka, Krajina, SG1-1, L94-117, L91-31022, Nena, ZPS-015, Lana and Novosadjanaka genotypes, respectively.
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f1-sensors-07-00067: PAGE analysis of the protein extracts. Lanes 1-12 represent the electrophoretic patterns of the Proteinka, Balkan, Ravnica, Vojvodjanka, Krajina, SG1-1, L94-117, L91-31022, Nena, ZPS-015, Lana and Novosadjanaka genotypes, respectively.

Mentions: PAGE separated total soybean proteins into several bands (Figure 1). According to our results, the Bowman-Birk type of trypsin inhibitors was detected in two zones, I and II, whereas the KTI was detected as one band. The zone with lower electrophoretic mobility represents the polymeric forms of BBI, whereas the other represents monomeric forms of BBI [7]. The presence of polymeric forms of BBI is result of their self-aggregation under non-dissociating conditions. [11, 25].


Influence of Different Genotypes on Trypsin Inhibitor Levels and Activity in Soybeans
PAGE analysis of the protein extracts. Lanes 1-12 represent the electrophoretic patterns of the Proteinka, Balkan, Ravnica, Vojvodjanka, Krajina, SG1-1, L94-117, L91-31022, Nena, ZPS-015, Lana and Novosadjanaka genotypes, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756712&req=5

f1-sensors-07-00067: PAGE analysis of the protein extracts. Lanes 1-12 represent the electrophoretic patterns of the Proteinka, Balkan, Ravnica, Vojvodjanka, Krajina, SG1-1, L94-117, L91-31022, Nena, ZPS-015, Lana and Novosadjanaka genotypes, respectively.
Mentions: PAGE separated total soybean proteins into several bands (Figure 1). According to our results, the Bowman-Birk type of trypsin inhibitors was detected in two zones, I and II, whereas the KTI was detected as one band. The zone with lower electrophoretic mobility represents the polymeric forms of BBI, whereas the other represents monomeric forms of BBI [7]. The presence of polymeric forms of BBI is result of their self-aggregation under non-dissociating conditions. [11, 25].

View Article: PubMed Central

ABSTRACT

This study describes the relationship between the two major trypsin inhibitors (TI) in soybean, i.e., the Kunitz (KTI) and Bowman-Birk (BBI) trypsin inhibitors, as well as between them and the corresponding trypsin inhibitor activity (TIA). Twelve investigated soybean genotypes showed significant differences in TI levels and TIA. A very strong positive correlation was found between the levels of KTI and total BBI (r = 0.94, P < 0.05). No relationship was found between KTI, BBI or total TI and TIA. Based on this data, it appears that the levels of major TI in soybean are related. Understanding the relationship between trypsin inhibitors and their activities could be useful for further improvement of the health impacts of soy proteins.

No MeSH data available.