Limits...
A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo

View Article: PubMed Central

ABSTRACT

In this paper, a new principle biosensor for non-invasive monitoring of the regulation of photosynthetic metabolism based on quantitative measurement of delayed fluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice as excitation light source and a compact Single Photon Counting Module to collect DF signal, is portable and can evaluate plant photosynthesis capacity in vivo. Compared with its primary version in our previous report, the biosensor can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light and CO2 response curves of DF intensity. In the experimental study, the testing of the improved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlings treated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism. Contrast evaluations of seedlings photosynthesis were made from measurements of net photosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testing results have demonstrated that the improved biosensor can accurately determine the regulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensor presented here could be potential useful for real-time monitoring the regulatory effects of plant growth regulators (PGRs) and other exogenous chemical factors on plant growth and photosynthetic metabolism.

No MeSH data available.


Light response curves of Pn (A) and DF intensity (B) of leaves of soybean (Glycine max Zaoshu No. 18) seedlings exposed to 1mM NaHSO3 concentration for 72 h. In the figure each value is the mean ± S.E. of nine independent leaves.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3756711&req=5

f3-sensors-07-00052: Light response curves of Pn (A) and DF intensity (B) of leaves of soybean (Glycine max Zaoshu No. 18) seedlings exposed to 1mM NaHSO3 concentration for 72 h. In the figure each value is the mean ± S.E. of nine independent leaves.

Mentions: To verify the new function of the biosensor for measuring the response of DF intensity to irradiance intensity, we contrastively analyzed the responses of Pn from LI-6400 and DF intensity from the biosensor to irradiance intensity after 1mM NaHSO3 treatment. Figure 3 shows the characteristics of the responsiveness of DF intensity and Pn of leaves of soybean seedlings exposed to 1 mM NaHSO3 for 72 h to irradiation intensity. It was clear that, at any given irradiance intensity, DF intensity and Pn, respectively, were higher in leaves with NaHSO3 treatment than in control at the nearly same extent (Figure 3). As irradiation intensity increased, both Pn and DF intensity increased linearly first, then reached a plateau at the same irradiation intensity of 700 μmol photons m−2 s−1 and leveled off with a further rise in irradiation intensity in both leaves with or without 1 mM NaHSO3 treatment (Figure 3). The results revealed that the changes in the light response curves of DF intensity from the biosensor were quite similar with that in the light response curves of Pn from LI-6400 even under NaHSO3 regulatory conditions.


A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo
Light response curves of Pn (A) and DF intensity (B) of leaves of soybean (Glycine max Zaoshu No. 18) seedlings exposed to 1mM NaHSO3 concentration for 72 h. In the figure each value is the mean ± S.E. of nine independent leaves.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756711&req=5

f3-sensors-07-00052: Light response curves of Pn (A) and DF intensity (B) of leaves of soybean (Glycine max Zaoshu No. 18) seedlings exposed to 1mM NaHSO3 concentration for 72 h. In the figure each value is the mean ± S.E. of nine independent leaves.
Mentions: To verify the new function of the biosensor for measuring the response of DF intensity to irradiance intensity, we contrastively analyzed the responses of Pn from LI-6400 and DF intensity from the biosensor to irradiance intensity after 1mM NaHSO3 treatment. Figure 3 shows the characteristics of the responsiveness of DF intensity and Pn of leaves of soybean seedlings exposed to 1 mM NaHSO3 for 72 h to irradiation intensity. It was clear that, at any given irradiance intensity, DF intensity and Pn, respectively, were higher in leaves with NaHSO3 treatment than in control at the nearly same extent (Figure 3). As irradiation intensity increased, both Pn and DF intensity increased linearly first, then reached a plateau at the same irradiation intensity of 700 μmol photons m−2 s−1 and leveled off with a further rise in irradiation intensity in both leaves with or without 1 mM NaHSO3 treatment (Figure 3). The results revealed that the changes in the light response curves of DF intensity from the biosensor were quite similar with that in the light response curves of Pn from LI-6400 even under NaHSO3 regulatory conditions.

View Article: PubMed Central

ABSTRACT

In this paper, a new principle biosensor for non-invasive monitoring of the regulation of photosynthetic metabolism based on quantitative measurement of delayed fluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice as excitation light source and a compact Single Photon Counting Module to collect DF signal, is portable and can evaluate plant photosynthesis capacity in vivo. Compared with its primary version in our previous report, the biosensor can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light and CO2 response curves of DF intensity. In the experimental study, the testing of the improved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlings treated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism. Contrast evaluations of seedlings photosynthesis were made from measurements of net photosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testing results have demonstrated that the improved biosensor can accurately determine the regulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensor presented here could be potential useful for real-time monitoring the regulatory effects of plant growth regulators (PGRs) and other exogenous chemical factors on plant growth and photosynthetic metabolism.

No MeSH data available.