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A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo

View Article: PubMed Central

ABSTRACT

In this paper, a new principle biosensor for non-invasive monitoring of the regulation of photosynthetic metabolism based on quantitative measurement of delayed fluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice as excitation light source and a compact Single Photon Counting Module to collect DF signal, is portable and can evaluate plant photosynthesis capacity in vivo. Compared with its primary version in our previous report, the biosensor can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light and CO2 response curves of DF intensity. In the experimental study, the testing of the improved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlings treated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism. Contrast evaluations of seedlings photosynthesis were made from measurements of net photosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testing results have demonstrated that the improved biosensor can accurately determine the regulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensor presented here could be potential useful for real-time monitoring the regulatory effects of plant growth regulators (PGRs) and other exogenous chemical factors on plant growth and photosynthetic metabolism.

No MeSH data available.


Effects of NaHSO3 on DF intensity and Pn of leaves of soybean (Glycine max Zaoshu No. 18) seedlings. (A) Changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations for 72 h. (B) The correlation between DF intensity and Pn (R = 0.996, P < 0.0001). In the figure each value is the mean ± S.E. of nine independent leaves.
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f2-sensors-07-00052: Effects of NaHSO3 on DF intensity and Pn of leaves of soybean (Glycine max Zaoshu No. 18) seedlings. (A) Changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations for 72 h. (B) The correlation between DF intensity and Pn (R = 0.996, P < 0.0001). In the figure each value is the mean ± S.E. of nine independent leaves.

Mentions: To test the accuracy of the developed biosensor, we first investigated the relationship between Pn from LI-6400 and DF intensity from the biosensor after different NaHSO3 concentrations treatment. Figure 2A shows the changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations (0, 0.5, 1, 2, 4, 8, 16 mM) for 72 h. As shown in Figure 2, the changes in DF intensity were highly consistent with that in Pn. DF and Pn increased as one man with the increasing of NaHSO3 concentrations upto 2 mM and decreased quickly at higher NaHSO3 concentrations. Moreover, DF and Pn at higher NaHSO3 concentration (> 4 mM) were less than that of control leaves (Figure 2A). This indicated that the photosynthetic efficiency of leaves of soybean was enhanced by the concentrations of NaHSO3 ranging between 0.5 and 2 mM. Only higher NaHSO3 concentrations (> 4 mM) could impair photosynthetic efficiency of leaf indicated by the changes in DF intensity and Pn (Figure 2A). The results are similar to that observed in wheat [3].


A Non-invasive and Real-time Monitoring of the Regulation of Photosynthetic Metabolism Biosensor Based on Measurement of Delayed Fluorescence in Vivo
Effects of NaHSO3 on DF intensity and Pn of leaves of soybean (Glycine max Zaoshu No. 18) seedlings. (A) Changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations for 72 h. (B) The correlation between DF intensity and Pn (R = 0.996, P < 0.0001). In the figure each value is the mean ± S.E. of nine independent leaves.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3756711&req=5

f2-sensors-07-00052: Effects of NaHSO3 on DF intensity and Pn of leaves of soybean (Glycine max Zaoshu No. 18) seedlings. (A) Changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations for 72 h. (B) The correlation between DF intensity and Pn (R = 0.996, P < 0.0001). In the figure each value is the mean ± S.E. of nine independent leaves.
Mentions: To test the accuracy of the developed biosensor, we first investigated the relationship between Pn from LI-6400 and DF intensity from the biosensor after different NaHSO3 concentrations treatment. Figure 2A shows the changes in DF intensity and Pn of leaves of soybean seedlings exposed to various NaHSO3 concentrations (0, 0.5, 1, 2, 4, 8, 16 mM) for 72 h. As shown in Figure 2, the changes in DF intensity were highly consistent with that in Pn. DF and Pn increased as one man with the increasing of NaHSO3 concentrations upto 2 mM and decreased quickly at higher NaHSO3 concentrations. Moreover, DF and Pn at higher NaHSO3 concentration (> 4 mM) were less than that of control leaves (Figure 2A). This indicated that the photosynthetic efficiency of leaves of soybean was enhanced by the concentrations of NaHSO3 ranging between 0.5 and 2 mM. Only higher NaHSO3 concentrations (> 4 mM) could impair photosynthetic efficiency of leaf indicated by the changes in DF intensity and Pn (Figure 2A). The results are similar to that observed in wheat [3].

View Article: PubMed Central

ABSTRACT

In this paper, a new principle biosensor for non-invasive monitoring of the regulation of photosynthetic metabolism based on quantitative measurement of delayed fluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice as excitation light source and a compact Single Photon Counting Module to collect DF signal, is portable and can evaluate plant photosynthesis capacity in vivo. Compared with its primary version in our previous report, the biosensor can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light and CO2 response curves of DF intensity. In the experimental study, the testing of the improved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlings treated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism. Contrast evaluations of seedlings photosynthesis were made from measurements of net photosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testing results have demonstrated that the improved biosensor can accurately determine the regulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensor presented here could be potential useful for real-time monitoring the regulatory effects of plant growth regulators (PGRs) and other exogenous chemical factors on plant growth and photosynthetic metabolism.

No MeSH data available.