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DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

View Article: PubMed Central

ABSTRACT

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mM range has been realized.

No MeSH data available.


A) Fringes shifts due to ssDNA-cDNA interaction. B) Dose-response curve as a function of the cDNA concentration.
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f5-sensors-07-00214: A) Fringes shifts due to ssDNA-cDNA interaction. B) Dose-response curve as a function of the cDNA concentration.

Mentions: In Figure 5A the reflectivity spectra of the PSi layer for different cDNA concentration are reported, while in Figure 5B a dose-response curve is reported. A control measurement has been made using a ncDNA sequence: a very small shift (less than 2 nm) has been recorded in the reflectivity spectrum respect to the one obtained after the probe linking.


DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips
A) Fringes shifts due to ssDNA-cDNA interaction. B) Dose-response curve as a function of the cDNA concentration.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756702&req=5

f5-sensors-07-00214: A) Fringes shifts due to ssDNA-cDNA interaction. B) Dose-response curve as a function of the cDNA concentration.
Mentions: In Figure 5A the reflectivity spectra of the PSi layer for different cDNA concentration are reported, while in Figure 5B a dose-response curve is reported. A control measurement has been made using a ncDNA sequence: a very small shift (less than 2 nm) has been recorded in the reflectivity spectrum respect to the one obtained after the probe linking.

View Article: PubMed Central

ABSTRACT

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mM range has been realized.

No MeSH data available.