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In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.

Pinhas A, Dubow M, Shah N, Chui TY, Scoles D, Sulai YN, Weitz R, Walsh JB, Carroll J, Dubra A, Rosen RB - Biomed Opt Express (2013)

Bottom Line: We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure.As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses.The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, New York Eye & Ear Infirmary, 310 E 14th St, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, 1428 Madison Ave, New York, NY 10029, USA ; Contributed equally to this manuscript and should be considered joint first authors.

ABSTRACT
The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

No MeSH data available.


Related in: MedlinePlus

Conventional SLO FA image acquired approximately 30 minutes after oral fluorescein administration (A) showing four peripapillary locations chosen for AOSLO imaging (A insets B, C, D and E). The reflectance AOSLO images of these small areas, focused on the nerve fiber layer (B1, C1, D1 and E1), show large blood vessels, capillaries and nerve fiber bundles. The corresponding AOSLO FA images recorded at the same focus (B2, C2, D2 and E2) approximately 15 minutes after oral fluorescein administration show only vasculature, revealing the radial peripapillary capillary bed with high contrast and detail. Peripapillary capillaries (B2, C2, D2 and E2) can be seen radiating parallel to the nerve fibers (B1, C1, D1 and E1). Capillary free zones can be seen surrounding arteries (B2, C2 and lower E2) and not veins (D2 and upper E2). AOSLO FA images focused closer to the outer retina (B3, C3, D3 and E3) show additional non-radial capillaries (arrow heads). These retinal capillaries can be seen deriving from the same retinal arteries (arrows) that supply the peripapillary capillaries. The scale bar represents 100 µm and applies to all images other than image A.
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g005: Conventional SLO FA image acquired approximately 30 minutes after oral fluorescein administration (A) showing four peripapillary locations chosen for AOSLO imaging (A insets B, C, D and E). The reflectance AOSLO images of these small areas, focused on the nerve fiber layer (B1, C1, D1 and E1), show large blood vessels, capillaries and nerve fiber bundles. The corresponding AOSLO FA images recorded at the same focus (B2, C2, D2 and E2) approximately 15 minutes after oral fluorescein administration show only vasculature, revealing the radial peripapillary capillary bed with high contrast and detail. Peripapillary capillaries (B2, C2, D2 and E2) can be seen radiating parallel to the nerve fibers (B1, C1, D1 and E1). Capillary free zones can be seen surrounding arteries (B2, C2 and lower E2) and not veins (D2 and upper E2). AOSLO FA images focused closer to the outer retina (B3, C3, D3 and E3) show additional non-radial capillaries (arrow heads). These retinal capillaries can be seen deriving from the same retinal arteries (arrows) that supply the peripapillary capillaries. The scale bar represents 100 µm and applies to all images other than image A.

Mentions: We examined the ability of AOSLO FA to axially resolve overlying capillary beds at 4 peripapillary locations (Fig. 5(A)Fig. 5


In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.

Pinhas A, Dubow M, Shah N, Chui TY, Scoles D, Sulai YN, Weitz R, Walsh JB, Carroll J, Dubra A, Rosen RB - Biomed Opt Express (2013)

Conventional SLO FA image acquired approximately 30 minutes after oral fluorescein administration (A) showing four peripapillary locations chosen for AOSLO imaging (A insets B, C, D and E). The reflectance AOSLO images of these small areas, focused on the nerve fiber layer (B1, C1, D1 and E1), show large blood vessels, capillaries and nerve fiber bundles. The corresponding AOSLO FA images recorded at the same focus (B2, C2, D2 and E2) approximately 15 minutes after oral fluorescein administration show only vasculature, revealing the radial peripapillary capillary bed with high contrast and detail. Peripapillary capillaries (B2, C2, D2 and E2) can be seen radiating parallel to the nerve fibers (B1, C1, D1 and E1). Capillary free zones can be seen surrounding arteries (B2, C2 and lower E2) and not veins (D2 and upper E2). AOSLO FA images focused closer to the outer retina (B3, C3, D3 and E3) show additional non-radial capillaries (arrow heads). These retinal capillaries can be seen deriving from the same retinal arteries (arrows) that supply the peripapillary capillaries. The scale bar represents 100 µm and applies to all images other than image A.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756583&req=5

g005: Conventional SLO FA image acquired approximately 30 minutes after oral fluorescein administration (A) showing four peripapillary locations chosen for AOSLO imaging (A insets B, C, D and E). The reflectance AOSLO images of these small areas, focused on the nerve fiber layer (B1, C1, D1 and E1), show large blood vessels, capillaries and nerve fiber bundles. The corresponding AOSLO FA images recorded at the same focus (B2, C2, D2 and E2) approximately 15 minutes after oral fluorescein administration show only vasculature, revealing the radial peripapillary capillary bed with high contrast and detail. Peripapillary capillaries (B2, C2, D2 and E2) can be seen radiating parallel to the nerve fibers (B1, C1, D1 and E1). Capillary free zones can be seen surrounding arteries (B2, C2 and lower E2) and not veins (D2 and upper E2). AOSLO FA images focused closer to the outer retina (B3, C3, D3 and E3) show additional non-radial capillaries (arrow heads). These retinal capillaries can be seen deriving from the same retinal arteries (arrows) that supply the peripapillary capillaries. The scale bar represents 100 µm and applies to all images other than image A.
Mentions: We examined the ability of AOSLO FA to axially resolve overlying capillary beds at 4 peripapillary locations (Fig. 5(A)Fig. 5

Bottom Line: We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure.As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses.The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, New York Eye & Ear Infirmary, 310 E 14th St, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, 1428 Madison Ave, New York, NY 10029, USA ; Contributed equally to this manuscript and should be considered joint first authors.

ABSTRACT
The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

No MeSH data available.


Related in: MedlinePlus