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In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.

Pinhas A, Dubow M, Shah N, Chui TY, Scoles D, Sulai YN, Weitz R, Walsh JB, Carroll J, Dubra A, Rosen RB - Biomed Opt Express (2013)

Bottom Line: We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure.As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses.The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, New York Eye & Ear Infirmary, 310 E 14th St, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, 1428 Madison Ave, New York, NY 10029, USA ; Contributed equally to this manuscript and should be considered joint first authors.

ABSTRACT
The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

No MeSH data available.


Related in: MedlinePlus

Comparison of AOSLO FA image sequences after oral or IV fluorescein administration, showing a retinal area approximately 5° inferotemporal from the fovea. Fluorescein was administered at time zero. Mean pixel value was calculated for each image and then normalized to the maximum value of the oral and IV image series to generate the intensity percentages, presented on the top right corner of each image. All images were then contrast stretched to make optimal use of the gray scale while avoiding saturation. The contrast-stretched images are presented in this figure. The scale bar represents 100 µm.
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g003: Comparison of AOSLO FA image sequences after oral or IV fluorescein administration, showing a retinal area approximately 5° inferotemporal from the fovea. Fluorescein was administered at time zero. Mean pixel value was calculated for each image and then normalized to the maximum value of the oral and IV image series to generate the intensity percentages, presented on the top right corner of each image. All images were then contrast stretched to make optimal use of the gray scale while avoiding saturation. The contrast-stretched images are presented in this figure. The scale bar represents 100 µm.

Mentions: To assess the strength of the fluorescence signal as a function of time in oral versus IV administration, we imaged the same patient with AOSLO FA in two different imaging sessions. Oral fluorescein (20 mg/kg) was used in the first session, and IV fluorescein (500 mg) was used in the second session. AOSLO imaging began 15 minutes post-administration of fluorescein and continued at 20-minute intervals until 115 minutes had elapsed (Fig. 3Fig. 3


In vivo imaging of human retinal microvasculature using adaptive optics scanning light ophthalmoscope fluorescein angiography.

Pinhas A, Dubow M, Shah N, Chui TY, Scoles D, Sulai YN, Weitz R, Walsh JB, Carroll J, Dubra A, Rosen RB - Biomed Opt Express (2013)

Comparison of AOSLO FA image sequences after oral or IV fluorescein administration, showing a retinal area approximately 5° inferotemporal from the fovea. Fluorescein was administered at time zero. Mean pixel value was calculated for each image and then normalized to the maximum value of the oral and IV image series to generate the intensity percentages, presented on the top right corner of each image. All images were then contrast stretched to make optimal use of the gray scale while avoiding saturation. The contrast-stretched images are presented in this figure. The scale bar represents 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756583&req=5

g003: Comparison of AOSLO FA image sequences after oral or IV fluorescein administration, showing a retinal area approximately 5° inferotemporal from the fovea. Fluorescein was administered at time zero. Mean pixel value was calculated for each image and then normalized to the maximum value of the oral and IV image series to generate the intensity percentages, presented on the top right corner of each image. All images were then contrast stretched to make optimal use of the gray scale while avoiding saturation. The contrast-stretched images are presented in this figure. The scale bar represents 100 µm.
Mentions: To assess the strength of the fluorescence signal as a function of time in oral versus IV administration, we imaged the same patient with AOSLO FA in two different imaging sessions. Oral fluorescein (20 mg/kg) was used in the first session, and IV fluorescein (500 mg) was used in the second session. AOSLO imaging began 15 minutes post-administration of fluorescein and continued at 20-minute intervals until 115 minutes had elapsed (Fig. 3Fig. 3

Bottom Line: We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure.As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses.The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, New York Eye & Ear Infirmary, 310 E 14th St, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, 1428 Madison Ave, New York, NY 10029, USA ; Contributed equally to this manuscript and should be considered joint first authors.

ABSTRACT
The adaptive optics scanning light ophthalmoscope (AOSLO) allows visualization of microscopic structures of the human retina in vivo. In this work, we demonstrate its application in combination with oral and intravenous (IV) fluorescein angiography (FA) to the in vivo visualization of the human retinal microvasculature. Ten healthy subjects ages 20 to 38 years were imaged using oral (7 and/or 20 mg/kg) and/or IV (500 mg) fluorescein. In agreement with current literature, there were no adverse effects among the patients receiving oral fluorescein while one patient receiving IV fluorescein experienced some nausea and heaving. We determined that all retinal capillary beds can be imaged using clinically accepted fluorescein dosages and safe light levels according to the ANSI Z136.1-2000 maximum permissible exposure. As expected, the 20 mg/kg oral dose showed higher image intensity for a longer period of time than did the 7 mg/kg oral and the 500 mg IV doses. The increased resolution of AOSLO FA, compared to conventional FA, offers great opportunity for studying physiological and pathological vascular processes.

No MeSH data available.


Related in: MedlinePlus