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Spermatological characteristics of Pleurogenidae (Digenea) inferred from the ultrastructural study of Pleurogenes claviger, Pleurogenoides medians and Prosotocus confusus.

Miquel J, Vilavella D, Swiderski Z, Shimalov VV, Torres J - Parasite (2013)

Bottom Line: The mature spermatozoa of these pleurogenids present two axonemes of the 9+"1" trepaxonematan pattern, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation, spine-like bodies and granules of glycogen.Thus, the anterior spermatozoon extremity is filiform and a continuous and submembranous layer of parallel cortical microtubules surrounds the axonemes at their anterior end.Slight differences were noted between the spermatozoon of P. confusus and those of the two remaining species in the location of mitochondria.

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Affiliation: Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, sn, 08028 Barcelona, Spain - Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.

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Spermatozoon of Pleurogenes claviger, posterior or nuclear part (Region III); all cross-sections. (a–c) Consecutive sections of the nuclear or posterior area of the sperm cell showing the appearance of the nucleus (N) and its progressively increasing size. CM, cortical microtubules; G, granules of glycogen; M2, second mitochondrion. (d) Absence of cortical microtubules. M2, second mitochondrion; N, nucleus. (e) Level of the disorganization of the first axoneme into doublets (D) and singlets (S). G, granules of glycogen; M2, second mitochondrion; N, nucleus. (f) Disappearance of the second mitochondrion and the reduction of the nucleus’ section (N). (g, h) Posterior spermatozoon extremity with and without granules of glycogen (G). Scales in μm: 0.3.
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Figure 2: Spermatozoon of Pleurogenes claviger, posterior or nuclear part (Region III); all cross-sections. (a–c) Consecutive sections of the nuclear or posterior area of the sperm cell showing the appearance of the nucleus (N) and its progressively increasing size. CM, cortical microtubules; G, granules of glycogen; M2, second mitochondrion. (d) Absence of cortical microtubules. M2, second mitochondrion; N, nucleus. (e) Level of the disorganization of the first axoneme into doublets (D) and singlets (S). G, granules of glycogen; M2, second mitochondrion; N, nucleus. (f) Disappearance of the second mitochondrion and the reduction of the nucleus’ section (N). (g, h) Posterior spermatozoon extremity with and without granules of glycogen (G). Scales in μm: 0.3.

Mentions: Posterior part or Region III (Figures 2a–h, 3l, 4a–h, 5m, n, 6a–h and 8III) corresponds to the nuclear region of the spermatozoon. Consecutive cross-sections show: (a) the increasing size of the nucleus (Figures 2a, b, 3l, 4a, and 5m, n); (b) the progressive reduction of cortical microtubules until their disappearance (Figures 2b–d, 4a, b, 6a, b and 8III); (c) the disorganization of the first axoneme (Figures 2e, 4c, 6c and 8III); (d) the end of second mitochondrion (Figures 2e, f, 4d, e, 5d, e and 8III) and (e) the reduction of nucleus size until its end (Figures 2f, g, 4e–g and 6e–g). Consequently, the posterior spermatozoon extremity of these species only exhibits the second axoneme (Figures 2g, h, 4h and 6g). Figure 4a clearly shows the transition of characters in the posterior spermatozoon extremity of P. medians, and Figure 6h shows the presence of some granules of glycogen in the posterior spermatozoon tip of P. confusus.


Spermatological characteristics of Pleurogenidae (Digenea) inferred from the ultrastructural study of Pleurogenes claviger, Pleurogenoides medians and Prosotocus confusus.

Miquel J, Vilavella D, Swiderski Z, Shimalov VV, Torres J - Parasite (2013)

Spermatozoon of Pleurogenes claviger, posterior or nuclear part (Region III); all cross-sections. (a–c) Consecutive sections of the nuclear or posterior area of the sperm cell showing the appearance of the nucleus (N) and its progressively increasing size. CM, cortical microtubules; G, granules of glycogen; M2, second mitochondrion. (d) Absence of cortical microtubules. M2, second mitochondrion; N, nucleus. (e) Level of the disorganization of the first axoneme into doublets (D) and singlets (S). G, granules of glycogen; M2, second mitochondrion; N, nucleus. (f) Disappearance of the second mitochondrion and the reduction of the nucleus’ section (N). (g, h) Posterior spermatozoon extremity with and without granules of glycogen (G). Scales in μm: 0.3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3756337&req=5

Figure 2: Spermatozoon of Pleurogenes claviger, posterior or nuclear part (Region III); all cross-sections. (a–c) Consecutive sections of the nuclear or posterior area of the sperm cell showing the appearance of the nucleus (N) and its progressively increasing size. CM, cortical microtubules; G, granules of glycogen; M2, second mitochondrion. (d) Absence of cortical microtubules. M2, second mitochondrion; N, nucleus. (e) Level of the disorganization of the first axoneme into doublets (D) and singlets (S). G, granules of glycogen; M2, second mitochondrion; N, nucleus. (f) Disappearance of the second mitochondrion and the reduction of the nucleus’ section (N). (g, h) Posterior spermatozoon extremity with and without granules of glycogen (G). Scales in μm: 0.3.
Mentions: Posterior part or Region III (Figures 2a–h, 3l, 4a–h, 5m, n, 6a–h and 8III) corresponds to the nuclear region of the spermatozoon. Consecutive cross-sections show: (a) the increasing size of the nucleus (Figures 2a, b, 3l, 4a, and 5m, n); (b) the progressive reduction of cortical microtubules until their disappearance (Figures 2b–d, 4a, b, 6a, b and 8III); (c) the disorganization of the first axoneme (Figures 2e, 4c, 6c and 8III); (d) the end of second mitochondrion (Figures 2e, f, 4d, e, 5d, e and 8III) and (e) the reduction of nucleus size until its end (Figures 2f, g, 4e–g and 6e–g). Consequently, the posterior spermatozoon extremity of these species only exhibits the second axoneme (Figures 2g, h, 4h and 6g). Figure 4a clearly shows the transition of characters in the posterior spermatozoon extremity of P. medians, and Figure 6h shows the presence of some granules of glycogen in the posterior spermatozoon tip of P. confusus.

Bottom Line: The mature spermatozoa of these pleurogenids present two axonemes of the 9+"1" trepaxonematan pattern, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation, spine-like bodies and granules of glycogen.Thus, the anterior spermatozoon extremity is filiform and a continuous and submembranous layer of parallel cortical microtubules surrounds the axonemes at their anterior end.Slight differences were noted between the spermatozoon of P. confusus and those of the two remaining species in the location of mitochondria.

View Article: PubMed Central - PubMed

Affiliation: Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, sn, 08028 Barcelona, Spain - Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.

Show MeSH
Related in: MedlinePlus