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Effects of Polybrominated Diphenol Ethers from a Marine Sponge Phyllospongia dendyi on IL-8 Production in a PMA-stimulated Promyelocytic Leukemia Cell Line

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ABSTRACT

The effects of five polybrominated diphenol ethers from a Marine Sponge, Phyllospongia dendyi, on the relative plating efficiencies in V79 cells and the cell proliferation and IL-8 production in PMA-stimulated HL-60 cells were examined. Three compounds, possessing two phenol units, were reported to inhibit the assembly of microtubule proteins and showed the inhibition of colony formation of V79 cells and increase in IL-8 production. Interestingly, a monomethoxy compound exhibited the stronger activity than the three active compounds in both bioassays. This compound did not inhibit the assembly of microtubule proteins. A dimethoxy compound was not active in either bioassays. In these experimental conditions, the biological activities were not high, but in future, these compounds will be expected to be made stronger by structural modifications.

No MeSH data available.


Related in: MedlinePlus

Effects of compounds 1–5 on IL-8 production and cell proliferation in PMA-stimulated HL-60 cells. A and B show the effects of compounds 1–5 on IL-8 production by 2 and 20 nM PMA added, respectively. HL-60 cells (1 x 106 cells/ml) were treated with PMA (2 or 20 nM) and the indicated concentration of each compound for 24 h. The IL-8 concentrations in the culture supernatant of the PMA-stimulated cells were determined to be ca. 14 and 40 ng/ml by ELISA, as described in the Materials and Methods. The data are shown as values relative (%) to the PMA-stimulated each IL-8 productions (14 and 40 ng/ml). The data are the mean values of three independent experiments.
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f3-marinedrugs-03-00119: Effects of compounds 1–5 on IL-8 production and cell proliferation in PMA-stimulated HL-60 cells. A and B show the effects of compounds 1–5 on IL-8 production by 2 and 20 nM PMA added, respectively. HL-60 cells (1 x 106 cells/ml) were treated with PMA (2 or 20 nM) and the indicated concentration of each compound for 24 h. The IL-8 concentrations in the culture supernatant of the PMA-stimulated cells were determined to be ca. 14 and 40 ng/ml by ELISA, as described in the Materials and Methods. The data are shown as values relative (%) to the PMA-stimulated each IL-8 productions (14 and 40 ng/ml). The data are the mean values of three independent experiments.

Mentions: To further examine the effects of compounds 1–5, we used PMA-stimulated HL-60 cells because of their IL-8 production. Comparison of the results of relative plating efficiencies and IL-8 production were very interesting, because IL-8 production correlated to the stoppage of cell growth. We examined the effects of compounds 1–5 on IL-8 production by the PMA (2 or 20 nM)-stimulated HL-60 cells, and the results are shown in Figure 3. The high concentration of compounds 1, 2, 3 and 5 showed the increases in the IL-8 production under the additional two doses of PMA; on the other hand, compound 4 did not increase the IL-8 production under the same experimental conditions. Moreover, the cell proliferations of each IL-8 production condition were determined by MTT methods (data not shown). The results, these proliferations at 10 and 50 μM of compounds 1, 2, 3 and 5 were inhibited strongly (20–80%). Thus, IL-8 production correlated with cell proliferation in this case.


Effects of Polybrominated Diphenol Ethers from a Marine Sponge Phyllospongia dendyi on IL-8 Production in a PMA-stimulated Promyelocytic Leukemia Cell Line
Effects of compounds 1–5 on IL-8 production and cell proliferation in PMA-stimulated HL-60 cells. A and B show the effects of compounds 1–5 on IL-8 production by 2 and 20 nM PMA added, respectively. HL-60 cells (1 x 106 cells/ml) were treated with PMA (2 or 20 nM) and the indicated concentration of each compound for 24 h. The IL-8 concentrations in the culture supernatant of the PMA-stimulated cells were determined to be ca. 14 and 40 ng/ml by ELISA, as described in the Materials and Methods. The data are shown as values relative (%) to the PMA-stimulated each IL-8 productions (14 and 40 ng/ml). The data are the mean values of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3756320&req=5

f3-marinedrugs-03-00119: Effects of compounds 1–5 on IL-8 production and cell proliferation in PMA-stimulated HL-60 cells. A and B show the effects of compounds 1–5 on IL-8 production by 2 and 20 nM PMA added, respectively. HL-60 cells (1 x 106 cells/ml) were treated with PMA (2 or 20 nM) and the indicated concentration of each compound for 24 h. The IL-8 concentrations in the culture supernatant of the PMA-stimulated cells were determined to be ca. 14 and 40 ng/ml by ELISA, as described in the Materials and Methods. The data are shown as values relative (%) to the PMA-stimulated each IL-8 productions (14 and 40 ng/ml). The data are the mean values of three independent experiments.
Mentions: To further examine the effects of compounds 1–5, we used PMA-stimulated HL-60 cells because of their IL-8 production. Comparison of the results of relative plating efficiencies and IL-8 production were very interesting, because IL-8 production correlated to the stoppage of cell growth. We examined the effects of compounds 1–5 on IL-8 production by the PMA (2 or 20 nM)-stimulated HL-60 cells, and the results are shown in Figure 3. The high concentration of compounds 1, 2, 3 and 5 showed the increases in the IL-8 production under the additional two doses of PMA; on the other hand, compound 4 did not increase the IL-8 production under the same experimental conditions. Moreover, the cell proliferations of each IL-8 production condition were determined by MTT methods (data not shown). The results, these proliferations at 10 and 50 μM of compounds 1, 2, 3 and 5 were inhibited strongly (20–80%). Thus, IL-8 production correlated with cell proliferation in this case.

View Article: PubMed Central

ABSTRACT

The effects of five polybrominated diphenol ethers from a Marine Sponge, Phyllospongia dendyi, on the relative plating efficiencies in V79 cells and the cell proliferation and IL-8 production in PMA-stimulated HL-60 cells were examined. Three compounds, possessing two phenol units, were reported to inhibit the assembly of microtubule proteins and showed the inhibition of colony formation of V79 cells and increase in IL-8 production. Interestingly, a monomethoxy compound exhibited the stronger activity than the three active compounds in both bioassays. This compound did not inhibit the assembly of microtubule proteins. A dimethoxy compound was not active in either bioassays. In these experimental conditions, the biological activities were not high, but in future, these compounds will be expected to be made stronger by structural modifications.

No MeSH data available.


Related in: MedlinePlus