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A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts.

Roh SS, Park SB, Park SM, Choi BW, Lee MH, Hwang YL, Kim CH, Jeong HA, Kim CD, Lee JH - Ann Dermatol (2013)

Bottom Line: Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts.Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin.Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Background: The fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.

Objective: This study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.

Methods: Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.

Results: Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.

Conclusion: These results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture.

No MeSH data available.


Related in: MedlinePlus

Effects of rasatiol on collagen and elastin production in dermal fibroblasts. (A, B) Cells were treated with rasatiol at the indicated concentrations for 2 d. A conditioned medium was collected, and the amounts of secreted procollagen type 1 and fibronectin were measured using ELISA. Rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid. Results are shown as a percentage of a control±the standard deviation (*p<0.05 versus control). (C) Cellular proteins were harvested and the protein levels for collagen type 1 α1 and elastin were verified using western blot analysis. Consistent with ELISA results, intracellular protein level of collagen type 1 α1 was increased in western blot analysis. Rasatiol also slightly increased the production of elastin. ELISA: enzyme-linked immunosorbent assay, Vit.: vitamin.
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Figure 3: Effects of rasatiol on collagen and elastin production in dermal fibroblasts. (A, B) Cells were treated with rasatiol at the indicated concentrations for 2 d. A conditioned medium was collected, and the amounts of secreted procollagen type 1 and fibronectin were measured using ELISA. Rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid. Results are shown as a percentage of a control±the standard deviation (*p<0.05 versus control). (C) Cellular proteins were harvested and the protein levels for collagen type 1 α1 and elastin were verified using western blot analysis. Consistent with ELISA results, intracellular protein level of collagen type 1 α1 was increased in western blot analysis. Rasatiol also slightly increased the production of elastin. ELISA: enzyme-linked immunosorbent assay, Vit.: vitamin.

Mentions: Since the proliferative property of fibroblasts is frequently linked to ECM production in fibroblasts, we next determined the effects of rasatiol on the production of collagen, fibronectin, and elastin. An ELISA assay showed that rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid (Fig, 3A, B). Consistent with this result, western blot analysis showed that rasatiol increased intracellular protein level of collagen type 1 α1 (Fig. 3C). Rasatiol also slightly increased the production of elastin, suggesting that rasatiol has a potential to activate fibroblasts.


A Novel Compound Rasatiol Isolated from Raphanus sativus Has a Potential to Enhance Extracellular Matrix Synthesis in Dermal Fibroblasts.

Roh SS, Park SB, Park SM, Choi BW, Lee MH, Hwang YL, Kim CH, Jeong HA, Kim CD, Lee JH - Ann Dermatol (2013)

Effects of rasatiol on collagen and elastin production in dermal fibroblasts. (A, B) Cells were treated with rasatiol at the indicated concentrations for 2 d. A conditioned medium was collected, and the amounts of secreted procollagen type 1 and fibronectin were measured using ELISA. Rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid. Results are shown as a percentage of a control±the standard deviation (*p<0.05 versus control). (C) Cellular proteins were harvested and the protein levels for collagen type 1 α1 and elastin were verified using western blot analysis. Consistent with ELISA results, intracellular protein level of collagen type 1 α1 was increased in western blot analysis. Rasatiol also slightly increased the production of elastin. ELISA: enzyme-linked immunosorbent assay, Vit.: vitamin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3756196&req=5

Figure 3: Effects of rasatiol on collagen and elastin production in dermal fibroblasts. (A, B) Cells were treated with rasatiol at the indicated concentrations for 2 d. A conditioned medium was collected, and the amounts of secreted procollagen type 1 and fibronectin were measured using ELISA. Rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid. Results are shown as a percentage of a control±the standard deviation (*p<0.05 versus control). (C) Cellular proteins were harvested and the protein levels for collagen type 1 α1 and elastin were verified using western blot analysis. Consistent with ELISA results, intracellular protein level of collagen type 1 α1 was increased in western blot analysis. Rasatiol also slightly increased the production of elastin. ELISA: enzyme-linked immunosorbent assay, Vit.: vitamin.
Mentions: Since the proliferative property of fibroblasts is frequently linked to ECM production in fibroblasts, we next determined the effects of rasatiol on the production of collagen, fibronectin, and elastin. An ELISA assay showed that rasatiol increased secretion of type 1 procollagen and fibronectin in a dose-dependent manner, and the effects were comparable with a positive control ascorbic acid (Fig, 3A, B). Consistent with this result, western blot analysis showed that rasatiol increased intracellular protein level of collagen type 1 α1 (Fig. 3C). Rasatiol also slightly increased the production of elastin, suggesting that rasatiol has a potential to activate fibroblasts.

Bottom Line: Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts.Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin.Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Background: The fibrous proteins of extracellular matrix (ECM) produced by dermal fibroblast contributes to the maintenance of connective tissue integrity.

Objective: This study is carried out to identify the bioactive ingredient from natural products that enhances ECM production in dermal fibroblasts.

Methods: Bioassay-directed fractionation was used to isolate the active ingredient from natural extracts. The effects of rasatiol (isolated from Raphanus sativus) on ECM production in primary cultured human dermal fibroblasts was investigated by enzyme linked immunosorbent assay and western blot analysis.

Results: Rasatiol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen, fibronectin and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was remarkably increased by rasatiol, indicating that enhanced ECM production is linked to the activation of intracellular signaling cascades.

Conclusion: These results indicate that rasatiol stimulates the fibrous components of ECM production, and may be applied to the maintenance of skin texture.

No MeSH data available.


Related in: MedlinePlus