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Evaluation of Expression of Lipases and Phospholipases of Malassezia restricta in Patients with Seborrheic Dermatitis.

Lee YW, Lee SY, Lee Y, Jung WH - Ann Dermatol (2013)

Bottom Line: The current study aimed to investigate the contribution of lipases and phospholipases in virulence of the M. restricta as being the most frequently isolated Malassezia spp. from the human skin.The results of the current study suggest that majority of the patients display expression of lipase RES_0242.These data imply a possible role of lipase in the host environment to produce free fatty acids for the fungus.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, School of Medicine, Konkuk University, Seoul, Korea.

ABSTRACT

Background: Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Almost all Malassezia spp. are obligatorily lipid-dependent, which might be caused by lack of the myristic acid synthesis. Recent genome analysis of M. restricta and M. globosa suggested that the absence of a gene encoding fatty acid synthesis might be compensated by abundant genes encoding hydrolases, which produce fatty acids, and that lipases and phospholipases may play a role in virulence of the fungus.

Objective: The current study aimed to investigate the contribution of lipases and phospholipases in virulence of the M. restricta as being the most frequently isolated Malassezia spp. from the human skin.

Methods: Swap samples of two different body sites of at least 18 patients with seborrheic dermatitis were obtained and in vivo expression of lipases and phospholipases of M. restricta was analyzed by the gene specific two-step nested RT-PCR.

Results: The results of the current study suggest that majority of the patients display expression of lipase RES_0242.

Conclusion: These data imply a possible role of lipase in the host environment to produce free fatty acids for the fungus.

No MeSH data available.


Related in: MedlinePlus

Summary of the results of two-step nested polymerase chain reaction. Asterisk indicates expression of the lipases and phospholipases in the cells cultured in vitro. F: female, M: male.
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Figure 2: Summary of the results of two-step nested polymerase chain reaction. Asterisk indicates expression of the lipases and phospholipases in the cells cultured in vitro. F: female, M: male.

Mentions: Using the two-step nested RT-PCR analysis, we were able to detect expression of the M. restrictaACT1 gene from the all swap samples except the cheeks of the patient 17. This result proved that M. restricta resides on the body sites of the patients tested in the current study. We also analyzed expression of lipases and phospholipases and found that 12 swap samples displayed expression of lipase RES_0242. Furthermore, our data showed that lipase RES_0571 was only expressed on the forehead of patient 13, and lipase RES_3114 was expressed on the forehead of patient 4 and on the cheeks of patient 7 and 10. A gene encoding phospholipase homolog RES_3947 was expressed on six swap samples (Fig. 1). Expression of the same lipases and phospholipases were also evaluated in the cells cultured in vitro. We found that lipase RES_0242 and lipase RES_3114 were not expressed in vitro and the results were summarized in Fig. 2. Thus, our data showed that majority of the patients displayed expression of lipase RES_0242 and suggested that the gene may play a significant role in the host environment such as contributing fatty acids production for M. restricta. Although it occurred from only three samples, lipase RES_3114 might also contribute to virulence since the gene was only expressed in vivo. Roles of phospholipase homolog RES_3947 and lipase RES_0571 were unclear because they were expressed in vitro.


Evaluation of Expression of Lipases and Phospholipases of Malassezia restricta in Patients with Seborrheic Dermatitis.

Lee YW, Lee SY, Lee Y, Jung WH - Ann Dermatol (2013)

Summary of the results of two-step nested polymerase chain reaction. Asterisk indicates expression of the lipases and phospholipases in the cells cultured in vitro. F: female, M: male.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3756195&req=5

Figure 2: Summary of the results of two-step nested polymerase chain reaction. Asterisk indicates expression of the lipases and phospholipases in the cells cultured in vitro. F: female, M: male.
Mentions: Using the two-step nested RT-PCR analysis, we were able to detect expression of the M. restrictaACT1 gene from the all swap samples except the cheeks of the patient 17. This result proved that M. restricta resides on the body sites of the patients tested in the current study. We also analyzed expression of lipases and phospholipases and found that 12 swap samples displayed expression of lipase RES_0242. Furthermore, our data showed that lipase RES_0571 was only expressed on the forehead of patient 13, and lipase RES_3114 was expressed on the forehead of patient 4 and on the cheeks of patient 7 and 10. A gene encoding phospholipase homolog RES_3947 was expressed on six swap samples (Fig. 1). Expression of the same lipases and phospholipases were also evaluated in the cells cultured in vitro. We found that lipase RES_0242 and lipase RES_3114 were not expressed in vitro and the results were summarized in Fig. 2. Thus, our data showed that majority of the patients displayed expression of lipase RES_0242 and suggested that the gene may play a significant role in the host environment such as contributing fatty acids production for M. restricta. Although it occurred from only three samples, lipase RES_3114 might also contribute to virulence since the gene was only expressed in vivo. Roles of phospholipase homolog RES_3947 and lipase RES_0571 were unclear because they were expressed in vitro.

Bottom Line: The current study aimed to investigate the contribution of lipases and phospholipases in virulence of the M. restricta as being the most frequently isolated Malassezia spp. from the human skin.The results of the current study suggest that majority of the patients display expression of lipase RES_0242.These data imply a possible role of lipase in the host environment to produce free fatty acids for the fungus.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, School of Medicine, Konkuk University, Seoul, Korea.

ABSTRACT

Background: Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Almost all Malassezia spp. are obligatorily lipid-dependent, which might be caused by lack of the myristic acid synthesis. Recent genome analysis of M. restricta and M. globosa suggested that the absence of a gene encoding fatty acid synthesis might be compensated by abundant genes encoding hydrolases, which produce fatty acids, and that lipases and phospholipases may play a role in virulence of the fungus.

Objective: The current study aimed to investigate the contribution of lipases and phospholipases in virulence of the M. restricta as being the most frequently isolated Malassezia spp. from the human skin.

Methods: Swap samples of two different body sites of at least 18 patients with seborrheic dermatitis were obtained and in vivo expression of lipases and phospholipases of M. restricta was analyzed by the gene specific two-step nested RT-PCR.

Results: The results of the current study suggest that majority of the patients display expression of lipase RES_0242.

Conclusion: These data imply a possible role of lipase in the host environment to produce free fatty acids for the fungus.

No MeSH data available.


Related in: MedlinePlus