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Effects of Xanthium stramarium and Psoralea corylifolia Extracts Combined with UVA1 Irradiation on the Cell Proliferation and TGF-β1 Expression of Keloid Fibroblasts.

Park SY, Park JY, Kim CH, Kang SU, Kim JH, Bark KM, Kim TH, Shin SC, Kang HY - Ann Dermatol (2013)

Bottom Line: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC), phototoxic oriental medicinal plants, has been used in traditional medicines in Asian countries.The effects of highly purified XAS or PSC extract combined with ultraviolet A1 (UVA1) irradiation on cell proliferation and transforming growth factor-beta1 (TGF-β1) expression of the keloid fibroblast were being investigated to define potential therapeutic uses for keloid treatments.The cell viability, apoptosis, and expression of TGF-β1 and collagen I were investigated.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.

ABSTRACT

Background: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC), phototoxic oriental medicinal plants, has been used in traditional medicines in Asian countries.

Objective: The effects of highly purified XAS or PSC extract combined with ultraviolet A1 (UVA1) irradiation on cell proliferation and transforming growth factor-beta1 (TGF-β1) expression of the keloid fibroblast were being investigated to define potential therapeutic uses for keloid treatments.

Methods: The keloid fibroblasts were treated with XAS or PSC alone or in the combination with UVA1 irradiation. The cell viability, apoptosis, and expression of TGF-β1 and collagen I were investigated.

Results: XAS and PSC in combination with UVA1 irradiation suppressed cell proliferation and induced apoptosis of keloid fibroblasts. Furthermore, the XAS and PSC in combination with UVA1 irradiation inhibited TGF-β1 expression and collagen synthesis in keloid fibroblasts.

Conclusion: These findings may open up the possibility of clinically used XAS or PSC in combination with UVA1 irradiation for keloid treatments.

No MeSH data available.


Related in: MedlinePlus

Xanthium stramarium (XAS) and Psoralea corylifolia (PSC) in combination with ultraviolet A1 (UVA1) irradiation suppressed transforming growth factor-beta1 (TGF-β1) expression and collagen I production in keloid fibroblasts. (A) The expression of TGF-β1 was analyzed by Enzyme-linked immunosorbent assay (ELISA). XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. #p<0.001, as compared with the control, *p<0.001, when compared with the control treated with UVA1 irradiation. The values represent mean±standard deviation of independent three experiments. (B) Reverse transcription-polymerase chain reaction (RT-PCR) study also showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 expression. (C) Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I expression.
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Figure 2: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC) in combination with ultraviolet A1 (UVA1) irradiation suppressed transforming growth factor-beta1 (TGF-β1) expression and collagen I production in keloid fibroblasts. (A) The expression of TGF-β1 was analyzed by Enzyme-linked immunosorbent assay (ELISA). XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. #p<0.001, as compared with the control, *p<0.001, when compared with the control treated with UVA1 irradiation. The values represent mean±standard deviation of independent three experiments. (B) Reverse transcription-polymerase chain reaction (RT-PCR) study also showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 expression. (C) Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I expression.

Mentions: TGF-β1 is an essential profibrotic cytokine for collagen synthesis, and administration of TGF-β1 resulted in a dramatic increase in intracellular collagen I levels in keloid fibroblasts11. To investigate the role of the treatment on the TGF-β1 and collagen expressions in keloid firboblasts, the ELISA analysis was first carried out (Fig. 2A). As a result, the XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. Reverse transcription-polymerase chain reaction study showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 mRNA expressions (Fig. 2B). Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I protein expression (Fig. 2C). These results suggest that XAC or PSC in combined with UVA1 irradiation also regulate collagen production by TGF-β1 in the keloid fibroblasts as well as suppressed keloid fibroblast viabilities. Interestingly, XAS or PSC itself showed inhibitory actions on TGF-β1 expression.


Effects of Xanthium stramarium and Psoralea corylifolia Extracts Combined with UVA1 Irradiation on the Cell Proliferation and TGF-β1 Expression of Keloid Fibroblasts.

Park SY, Park JY, Kim CH, Kang SU, Kim JH, Bark KM, Kim TH, Shin SC, Kang HY - Ann Dermatol (2013)

Xanthium stramarium (XAS) and Psoralea corylifolia (PSC) in combination with ultraviolet A1 (UVA1) irradiation suppressed transforming growth factor-beta1 (TGF-β1) expression and collagen I production in keloid fibroblasts. (A) The expression of TGF-β1 was analyzed by Enzyme-linked immunosorbent assay (ELISA). XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. #p<0.001, as compared with the control, *p<0.001, when compared with the control treated with UVA1 irradiation. The values represent mean±standard deviation of independent three experiments. (B) Reverse transcription-polymerase chain reaction (RT-PCR) study also showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 expression. (C) Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I expression.
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Related In: Results  -  Collection

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Figure 2: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC) in combination with ultraviolet A1 (UVA1) irradiation suppressed transforming growth factor-beta1 (TGF-β1) expression and collagen I production in keloid fibroblasts. (A) The expression of TGF-β1 was analyzed by Enzyme-linked immunosorbent assay (ELISA). XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. #p<0.001, as compared with the control, *p<0.001, when compared with the control treated with UVA1 irradiation. The values represent mean±standard deviation of independent three experiments. (B) Reverse transcription-polymerase chain reaction (RT-PCR) study also showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 expression. (C) Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I expression.
Mentions: TGF-β1 is an essential profibrotic cytokine for collagen synthesis, and administration of TGF-β1 resulted in a dramatic increase in intracellular collagen I levels in keloid fibroblasts11. To investigate the role of the treatment on the TGF-β1 and collagen expressions in keloid firboblasts, the ELISA analysis was first carried out (Fig. 2A). As a result, the XAS (50 µg/ml) or PSC (10 µg/ml) treatment in combination with UVA1 irradiation inhibited the TGF-β1 expression in the keloid fibroblasts. The inhibitory effects of the XAS and PSC in combination with UVA1 were better than the effects of psoralen in combination with UVA1. Reverse transcription-polymerase chain reaction study showed that XAS (50 µg/ml) or PSC (10 µg/ml) reduced collagen I and TGF-β1 mRNA expressions (Fig. 2B). Western blotting further confirmed the inhibitory actions of XAS or PSC in combination with UVA1 irradiation on collagen I protein expression (Fig. 2C). These results suggest that XAC or PSC in combined with UVA1 irradiation also regulate collagen production by TGF-β1 in the keloid fibroblasts as well as suppressed keloid fibroblast viabilities. Interestingly, XAS or PSC itself showed inhibitory actions on TGF-β1 expression.

Bottom Line: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC), phototoxic oriental medicinal plants, has been used in traditional medicines in Asian countries.The effects of highly purified XAS or PSC extract combined with ultraviolet A1 (UVA1) irradiation on cell proliferation and transforming growth factor-beta1 (TGF-β1) expression of the keloid fibroblast were being investigated to define potential therapeutic uses for keloid treatments.The cell viability, apoptosis, and expression of TGF-β1 and collagen I were investigated.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.

ABSTRACT

Background: Xanthium stramarium (XAS) and Psoralea corylifolia (PSC), phototoxic oriental medicinal plants, has been used in traditional medicines in Asian countries.

Objective: The effects of highly purified XAS or PSC extract combined with ultraviolet A1 (UVA1) irradiation on cell proliferation and transforming growth factor-beta1 (TGF-β1) expression of the keloid fibroblast were being investigated to define potential therapeutic uses for keloid treatments.

Methods: The keloid fibroblasts were treated with XAS or PSC alone or in the combination with UVA1 irradiation. The cell viability, apoptosis, and expression of TGF-β1 and collagen I were investigated.

Results: XAS and PSC in combination with UVA1 irradiation suppressed cell proliferation and induced apoptosis of keloid fibroblasts. Furthermore, the XAS and PSC in combination with UVA1 irradiation inhibited TGF-β1 expression and collagen synthesis in keloid fibroblasts.

Conclusion: These findings may open up the possibility of clinically used XAS or PSC in combination with UVA1 irradiation for keloid treatments.

No MeSH data available.


Related in: MedlinePlus