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The allergenic potency of Japanese hop pollen is increasing with environmental changes in Korea.

Jin HJ, Choi GS, Shin YS, Kim JH, Kim JE, Ye YM, Park HS - Allergy Asthma Immunol Res (2013)

Bottom Line: IgE binding components were compared by IgE immunoblot analysis.Serum specific IgE levels to the 09 and urban extracts in both groups increased significantly compared to those of the 98 and suburban extracts.When the 98 and 09 extracts were compared, intensification of the major allergen of 09 extract had occurred in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Yeungnam University College of Medicine, Daegu, Korea. ; Center for Respiratory Disease, Yeungnam University Medical Center, Daegu, Korea.

ABSTRACT

Purpose: The sensitization rate to Japanese Hop (Hop J) in respiratory allergy patients has increased in recent years in Korea. We evaluated changes in the allergenic potency of Hop J pollen collected in 1998 and 2009.

Methods: Thirty-five patients with allergic rhinitis and/or asthma were enrolled. Group I included 21 subjects sensitized to Hop J at an initial visit and group II included 14 subjects who developed a new sensitization. Hop J pollens were collected in 1998 and 2009 (98 and 09 extracts) and both urban and suburban environments (urban and suburban extracts). Serum specific IgE levels to Hop J pollen extracts were compared using an enzyme-linked immunosorbent assay (ELISA). IgE binding components were compared by IgE immunoblot analysis.

Results: Serum specific IgE levels to the 09 and urban extracts in both groups increased significantly compared to those of the 98 and suburban extracts. IgE immunoblot demonstrated that the major 10 kDa allergen was intensified in group I, while it was newly generated in group II with additional components ranging from 12-95 kDa. When the 98 and 09 extracts were compared, intensification of the major allergen of 09 extract had occurred in both groups. The IgE binding components of the urban extract was stronger than those of suburban one.

Conclusions: The allergenic potency of Hop J pollen may be increased with environmental changes.

No MeSH data available.


Related in: MedlinePlus

Changes in specific IgE binding components as determined by immunoblot using the Urban and Suburban extracts in Group I (A) and Group II (B). a, Suburban Hop J pollen extracts; b, Urban Hop J pollen extracts.
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Figure 3: Changes in specific IgE binding components as determined by immunoblot using the Urban and Suburban extracts in Group I (A) and Group II (B). a, Suburban Hop J pollen extracts; b, Urban Hop J pollen extracts.

Mentions: Fig. 2 shows the distribution of the IgE binding component in group I, in which a major IgE binding component at 10 kDa was detected. In addition, this component was newly generated (data not shown), and other IgE binding components ranging from 12-95 kDa were generated in group II. When these IgE binding components were compared between the 98 and 09 extracts in groups I and II, the intensity of the major IgE binding component at 10 kDa was further intensified (1.39 and 1.79×the mean optical density increased in group I and II) in the 09 extract in both groups. The new IgE binding components were more prominent within the 09 extract. In addition, when we compared the 09 Hop J extracts derived from urban and suburban areas, both the major 10 kDa IgE binding component and the new IgE binding components (12-95 kDa) were enhanced in the urban extracts in both groups (Fig. 3).


The allergenic potency of Japanese hop pollen is increasing with environmental changes in Korea.

Jin HJ, Choi GS, Shin YS, Kim JH, Kim JE, Ye YM, Park HS - Allergy Asthma Immunol Res (2013)

Changes in specific IgE binding components as determined by immunoblot using the Urban and Suburban extracts in Group I (A) and Group II (B). a, Suburban Hop J pollen extracts; b, Urban Hop J pollen extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3756178&req=5

Figure 3: Changes in specific IgE binding components as determined by immunoblot using the Urban and Suburban extracts in Group I (A) and Group II (B). a, Suburban Hop J pollen extracts; b, Urban Hop J pollen extracts.
Mentions: Fig. 2 shows the distribution of the IgE binding component in group I, in which a major IgE binding component at 10 kDa was detected. In addition, this component was newly generated (data not shown), and other IgE binding components ranging from 12-95 kDa were generated in group II. When these IgE binding components were compared between the 98 and 09 extracts in groups I and II, the intensity of the major IgE binding component at 10 kDa was further intensified (1.39 and 1.79×the mean optical density increased in group I and II) in the 09 extract in both groups. The new IgE binding components were more prominent within the 09 extract. In addition, when we compared the 09 Hop J extracts derived from urban and suburban areas, both the major 10 kDa IgE binding component and the new IgE binding components (12-95 kDa) were enhanced in the urban extracts in both groups (Fig. 3).

Bottom Line: IgE binding components were compared by IgE immunoblot analysis.Serum specific IgE levels to the 09 and urban extracts in both groups increased significantly compared to those of the 98 and suburban extracts.When the 98 and 09 extracts were compared, intensification of the major allergen of 09 extract had occurred in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Yeungnam University College of Medicine, Daegu, Korea. ; Center for Respiratory Disease, Yeungnam University Medical Center, Daegu, Korea.

ABSTRACT

Purpose: The sensitization rate to Japanese Hop (Hop J) in respiratory allergy patients has increased in recent years in Korea. We evaluated changes in the allergenic potency of Hop J pollen collected in 1998 and 2009.

Methods: Thirty-five patients with allergic rhinitis and/or asthma were enrolled. Group I included 21 subjects sensitized to Hop J at an initial visit and group II included 14 subjects who developed a new sensitization. Hop J pollens were collected in 1998 and 2009 (98 and 09 extracts) and both urban and suburban environments (urban and suburban extracts). Serum specific IgE levels to Hop J pollen extracts were compared using an enzyme-linked immunosorbent assay (ELISA). IgE binding components were compared by IgE immunoblot analysis.

Results: Serum specific IgE levels to the 09 and urban extracts in both groups increased significantly compared to those of the 98 and suburban extracts. IgE immunoblot demonstrated that the major 10 kDa allergen was intensified in group I, while it was newly generated in group II with additional components ranging from 12-95 kDa. When the 98 and 09 extracts were compared, intensification of the major allergen of 09 extract had occurred in both groups. The IgE binding components of the urban extract was stronger than those of suburban one.

Conclusions: The allergenic potency of Hop J pollen may be increased with environmental changes.

No MeSH data available.


Related in: MedlinePlus