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Epigenetic regulation in the brain after spinal cord injury : a comparative study.

Park BN, Kim SW, Cho SR, Lee JY, Lee YH, Kim SH - J Korean Neurosurg Soc (2013)

Bottom Line: The expression of NGF levels were elevated at 2, 4 weeks compared with control group, but these difference were not significant (p>0.05).The GDNF levels were elevated at 2 week compared with control group, but these differences were not significant (p>0.05).The difference of HDAC1 levels were not significant at 2, 4 and 8 weeks compared with control group (p>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Wonju Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea.

ABSTRACT

Objective: After spinal cord injury (SCI), functional and structural reorganization occurs at multiple levels of brain including motor cortex. However, the underlying mechanism still remains unclear. The current study was performed to investigate the alterations in the expression of the main regulators of neuronal development, survival and death, in the brain following thoracic contusive SCI in a mouse model.

Methods: Eight-week-old female imprinting control region mice (n=60; 30-35 g) were used in this study. We analyzed the expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF) and histone deacetylase (HDAC) 1 in the brain following thoracic contusive SCI.

Results: The expression of BDNF levels were elevated significantly compared with control group at 2 weeks after injury (p<0.05). The expression of NGF levels were elevated at 2, 4 weeks compared with control group, but these difference were not significant (p>0.05). The GDNF levels were elevated at 2 week compared with control group, but these differences were not significant (p>0.05). The difference of HDAC1 levels were not significant at 2, 4 and 8 weeks compared with control group (p>0.05).

Conclusion: These results demonstrate that the upregulation of BDNF may play on important role in brain reorganization after SCI.

No MeSH data available.


Related in: MedlinePlus

Semi-quantitative immunoblot analysis of GDNF expression in brain after contusion of the spinal cord in mice (A and B). GDNF elevated compared with those of control group at 2 weeks, however the difference is not significant (p>0.05). In this figure (B) data are plotted as mean±standard error of the mean. GDNF : glial cell line-derived neurotrophic factor, SCI : spinal cord injury, CONT : control, GAPDH : glyceraldehyde 3-phosphate dehydrogenase.
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Figure 4: Semi-quantitative immunoblot analysis of GDNF expression in brain after contusion of the spinal cord in mice (A and B). GDNF elevated compared with those of control group at 2 weeks, however the difference is not significant (p>0.05). In this figure (B) data are plotted as mean±standard error of the mean. GDNF : glial cell line-derived neurotrophic factor, SCI : spinal cord injury, CONT : control, GAPDH : glyceraldehyde 3-phosphate dehydrogenase.

Mentions: The level of GDNF was elevated in the SCI group at 2 weeks (1.16±0.11) and 8 weeks (1.01±0.09) compared with those of control group after SCI, however the differences were not significant (p>0.05) (Fig. 4).


Epigenetic regulation in the brain after spinal cord injury : a comparative study.

Park BN, Kim SW, Cho SR, Lee JY, Lee YH, Kim SH - J Korean Neurosurg Soc (2013)

Semi-quantitative immunoblot analysis of GDNF expression in brain after contusion of the spinal cord in mice (A and B). GDNF elevated compared with those of control group at 2 weeks, however the difference is not significant (p>0.05). In this figure (B) data are plotted as mean±standard error of the mean. GDNF : glial cell line-derived neurotrophic factor, SCI : spinal cord injury, CONT : control, GAPDH : glyceraldehyde 3-phosphate dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3756125&req=5

Figure 4: Semi-quantitative immunoblot analysis of GDNF expression in brain after contusion of the spinal cord in mice (A and B). GDNF elevated compared with those of control group at 2 weeks, however the difference is not significant (p>0.05). In this figure (B) data are plotted as mean±standard error of the mean. GDNF : glial cell line-derived neurotrophic factor, SCI : spinal cord injury, CONT : control, GAPDH : glyceraldehyde 3-phosphate dehydrogenase.
Mentions: The level of GDNF was elevated in the SCI group at 2 weeks (1.16±0.11) and 8 weeks (1.01±0.09) compared with those of control group after SCI, however the differences were not significant (p>0.05) (Fig. 4).

Bottom Line: The expression of NGF levels were elevated at 2, 4 weeks compared with control group, but these difference were not significant (p>0.05).The GDNF levels were elevated at 2 week compared with control group, but these differences were not significant (p>0.05).The difference of HDAC1 levels were not significant at 2, 4 and 8 weeks compared with control group (p>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Wonju Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea.

ABSTRACT

Objective: After spinal cord injury (SCI), functional and structural reorganization occurs at multiple levels of brain including motor cortex. However, the underlying mechanism still remains unclear. The current study was performed to investigate the alterations in the expression of the main regulators of neuronal development, survival and death, in the brain following thoracic contusive SCI in a mouse model.

Methods: Eight-week-old female imprinting control region mice (n=60; 30-35 g) were used in this study. We analyzed the expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF) and histone deacetylase (HDAC) 1 in the brain following thoracic contusive SCI.

Results: The expression of BDNF levels were elevated significantly compared with control group at 2 weeks after injury (p<0.05). The expression of NGF levels were elevated at 2, 4 weeks compared with control group, but these difference were not significant (p>0.05). The GDNF levels were elevated at 2 week compared with control group, but these differences were not significant (p>0.05). The difference of HDAC1 levels were not significant at 2, 4 and 8 weeks compared with control group (p>0.05).

Conclusion: These results demonstrate that the upregulation of BDNF may play on important role in brain reorganization after SCI.

No MeSH data available.


Related in: MedlinePlus