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Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

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Histological evaluation of anti-inflammatory effects of Gelam honey.Hematoxylin and Eosin staining of paw tissues of rats pre-treated with: (A) D.W., (B) 2 g/kg Gelam honey, (C) D.W.+Carr., (D) 2 g/kg Gelam honey for 1 day+Carr. (E) 2 g/kg Gelam honey for 7 days+Carr. and (F) 10 mg/kg IND.+Carr., in the model of carrageenan-induced edema. Each photo is representative of six specimens for each group. All figures were magnified by 200X. (G) Scores of inflammation in rats paw tissues by Hematoxylin and Eosin staining. Data are expressed as mean ± S.E.M. (n = 6). D.W: Distilled water, Carr.: Carrageenan and IND: Indomethacin. a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days). The arrows indicated inflammatory cells.
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pone-0072365-g004: Histological evaluation of anti-inflammatory effects of Gelam honey.Hematoxylin and Eosin staining of paw tissues of rats pre-treated with: (A) D.W., (B) 2 g/kg Gelam honey, (C) D.W.+Carr., (D) 2 g/kg Gelam honey for 1 day+Carr. (E) 2 g/kg Gelam honey for 7 days+Carr. and (F) 10 mg/kg IND.+Carr., in the model of carrageenan-induced edema. Each photo is representative of six specimens for each group. All figures were magnified by 200X. (G) Scores of inflammation in rats paw tissues by Hematoxylin and Eosin staining. Data are expressed as mean ± S.E.M. (n = 6). D.W: Distilled water, Carr.: Carrageenan and IND: Indomethacin. a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days). The arrows indicated inflammatory cells.

Mentions: To evaluate histologically the anti-inflammatory effect of Gelam honey, samples of the paw tissues from each experimental group were examined by H&E staining. The control groups which were fed with distilled water and Gelam honey at 1 or 2 g/kg of body weight (not induced with inflammation) showed normal paw tissue histology (Figures 4A & 4B). In contrast, the carrageenan injection into the rat right hind paw displayed massive accumulation of infiltrated inflammatory cells (Figure 4C), compared to the control groups. However, the infiltration of inflammatory cells was significantly decreased with treatment of Gelam honey (1 or 2 g/kg of body weight) or Indomethacin (10 mg/kg of body weight), for both 1 and 7 days model (Figures 4D–4F). The degree of inflammation was evaluated by scores of inflammation from 0 to 5 (Figure 4G). The scores of inflammation also indicated that pre-treatment with Gelam honey (1 & 2 g/kg of body weight) for 7 days had greater effect in reducing the inflammatory cells in carrageenan-induced inflammation rats compared to 1 day.


Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

Histological evaluation of anti-inflammatory effects of Gelam honey.Hematoxylin and Eosin staining of paw tissues of rats pre-treated with: (A) D.W., (B) 2 g/kg Gelam honey, (C) D.W.+Carr., (D) 2 g/kg Gelam honey for 1 day+Carr. (E) 2 g/kg Gelam honey for 7 days+Carr. and (F) 10 mg/kg IND.+Carr., in the model of carrageenan-induced edema. Each photo is representative of six specimens for each group. All figures were magnified by 200X. (G) Scores of inflammation in rats paw tissues by Hematoxylin and Eosin staining. Data are expressed as mean ± S.E.M. (n = 6). D.W: Distilled water, Carr.: Carrageenan and IND: Indomethacin. a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days). The arrows indicated inflammatory cells.
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pone-0072365-g004: Histological evaluation of anti-inflammatory effects of Gelam honey.Hematoxylin and Eosin staining of paw tissues of rats pre-treated with: (A) D.W., (B) 2 g/kg Gelam honey, (C) D.W.+Carr., (D) 2 g/kg Gelam honey for 1 day+Carr. (E) 2 g/kg Gelam honey for 7 days+Carr. and (F) 10 mg/kg IND.+Carr., in the model of carrageenan-induced edema. Each photo is representative of six specimens for each group. All figures were magnified by 200X. (G) Scores of inflammation in rats paw tissues by Hematoxylin and Eosin staining. Data are expressed as mean ± S.E.M. (n = 6). D.W: Distilled water, Carr.: Carrageenan and IND: Indomethacin. a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days). The arrows indicated inflammatory cells.
Mentions: To evaluate histologically the anti-inflammatory effect of Gelam honey, samples of the paw tissues from each experimental group were examined by H&E staining. The control groups which were fed with distilled water and Gelam honey at 1 or 2 g/kg of body weight (not induced with inflammation) showed normal paw tissue histology (Figures 4A & 4B). In contrast, the carrageenan injection into the rat right hind paw displayed massive accumulation of infiltrated inflammatory cells (Figure 4C), compared to the control groups. However, the infiltration of inflammatory cells was significantly decreased with treatment of Gelam honey (1 or 2 g/kg of body weight) or Indomethacin (10 mg/kg of body weight), for both 1 and 7 days model (Figures 4D–4F). The degree of inflammation was evaluated by scores of inflammation from 0 to 5 (Figure 4G). The scores of inflammation also indicated that pre-treatment with Gelam honey (1 & 2 g/kg of body weight) for 7 days had greater effect in reducing the inflammatory cells in carrageenan-induced inflammation rats compared to 1 day.

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

Show MeSH
Related in: MedlinePlus