Limits...
Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

Show MeSH

Related in: MedlinePlus

The effect of Gelam honey on the cytosolic IκBα protein expression in rats paw tissues.Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3756081&req=5

pone-0072365-g003: The effect of Gelam honey on the cytosolic IκBα protein expression in rats paw tissues.Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days).

Mentions: Figure 3 showed that carrageenan injection induced the cytoplasmic degradation of IκBα protein showing low levels of IκBα when compared with the control groups (distilled water and two doses of Gelam honey) not induced with inflammation which exhibited high level of cytoplasmic IκBα protein. However, the cytoplasmic level of IκBα increased significantly by the pre-treatment with Gelam honey (1 and 2 g/kg of body weight) in both 1 and 7 days models. Interestingly, pre-treatment with 2 g/kg of body weight of Gelam honey inhibited the IκBα cytoplasmic degradation dose dependently showing more levels of cytosolic IκBα compared to the effect of lower dose of Gelam honey (1 g/kg of body weight) in both 1 and 7 days models (Figure 3).


Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

The effect of Gelam honey on the cytosolic IκBα protein expression in rats paw tissues.Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756081&req=5

pone-0072365-g003: The effect of Gelam honey on the cytosolic IκBα protein expression in rats paw tissues.Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days).
Mentions: Figure 3 showed that carrageenan injection induced the cytoplasmic degradation of IκBα protein showing low levels of IκBα when compared with the control groups (distilled water and two doses of Gelam honey) not induced with inflammation which exhibited high level of cytoplasmic IκBα protein. However, the cytoplasmic level of IκBα increased significantly by the pre-treatment with Gelam honey (1 and 2 g/kg of body weight) in both 1 and 7 days models. Interestingly, pre-treatment with 2 g/kg of body weight of Gelam honey inhibited the IκBα cytoplasmic degradation dose dependently showing more levels of cytosolic IκBα compared to the effect of lower dose of Gelam honey (1 g/kg of body weight) in both 1 and 7 days models (Figure 3).

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

Show MeSH
Related in: MedlinePlus