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Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

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The effect of Gelam honey on the protein expressions in rats paw tissues.(A) Cytosolic NF-κB subunits p65 & p50 and (B) Nuclear NF-κB subunits p65 & p50. Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days) and same protein (p65 or p50). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days) and same protein (p65 or p50).
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pone-0072365-g002: The effect of Gelam honey on the protein expressions in rats paw tissues.(A) Cytosolic NF-κB subunits p65 & p50 and (B) Nuclear NF-κB subunits p65 & p50. Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days) and same protein (p65 or p50). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days) and same protein (p65 or p50).

Mentions: The results demonstrated that carrageenan injection induced the nuclear translocation of NF-κB subunits p65 and p50, as shown in Figures 2A & 2B. However, the cytoplasmic level of p65 and p50 increased significantly by the pre-treatment with Gelam honey (1 & 2 g/kg of body weight) in both 1 and 7 days models (Figure 2A). Simultaneously, the nuclear levels of p65 and p50 decreased significantly with Gelam honey pre-treatment (1 & 2 g/kg of body weight) for 1 and 7 days (Figure 2B), with concomitant increased of their cytoplasmic levels (Figure 2A). Pre-treatment with 2 g/kg of body weight of Gelam honey for 7 days showed greater effect in decreasing the nuclear p65 and p50 levels in parallel with their increased cytosolic levels.


Gelam honey attenuates carrageenan-induced rat paw inflammation via NF-κB pathway.

Hussein SZ, Mohd Yusoff K, Makpol S, Mohd Yusof YA - PLoS ONE (2013)

The effect of Gelam honey on the protein expressions in rats paw tissues.(A) Cytosolic NF-κB subunits p65 & p50 and (B) Nuclear NF-κB subunits p65 & p50. Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days) and same protein (p65 or p50). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days) and same protein (p65 or p50).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3756081&req=5

pone-0072365-g002: The effect of Gelam honey on the protein expressions in rats paw tissues.(A) Cytosolic NF-κB subunits p65 & p50 and (B) Nuclear NF-κB subunits p65 & p50. Rats were pretreated orally with Gelam honey (1 or 2 g/kg of body weight) for 1 or 7 days before inflammation, as induced by carrageenan injection. D.W.: distilled water, Carr: Carrageenan, IND: Indomethacin. Data are presented as the mean ± S.E.M. (n = 6). a: Significantly different (p<0.05) from the inflammation group (D.W.+Carr.). b: Significantly different (p<0.05) from the Indomethacin group (10 mg/kg IND+Carr.). c: Significantly different (p<0.05) between different honey doses at the same time point (1 day or 7 days) and same protein (p65 or p50). d: Significantly different (p<0.05) between the same honey dose at different time points (1 day and 7 days) and same protein (p65 or p50).
Mentions: The results demonstrated that carrageenan injection induced the nuclear translocation of NF-κB subunits p65 and p50, as shown in Figures 2A & 2B. However, the cytoplasmic level of p65 and p50 increased significantly by the pre-treatment with Gelam honey (1 & 2 g/kg of body weight) in both 1 and 7 days models (Figure 2A). Simultaneously, the nuclear levels of p65 and p50 decreased significantly with Gelam honey pre-treatment (1 & 2 g/kg of body weight) for 1 and 7 days (Figure 2B), with concomitant increased of their cytoplasmic levels (Figure 2A). Pre-treatment with 2 g/kg of body weight of Gelam honey for 7 days showed greater effect in decreasing the nuclear p65 and p50 levels in parallel with their increased cytosolic levels.

Bottom Line: Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues.The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey.The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
The activation of nuclear factor kappa B (NF-κB) plays a major role in the pathogenesis of a number of inflammatory diseases. In this study, we investigated the anti-inflammatory mechanism of Gelam honey in inflammation induced rats via NF-κB signalling pathway. Rats paw edema was induced by subplantar injection of 1% carrageenan into the right hind paw. Rats were pre-treated with Gelam honey at different doses (1 or 2 g/kg, p.o.) and NSAID Indomethacin (10 mg/kg, p.o.), in two time points (1 and 7 days). Our results showed that Gelam honey at both concentrations suppressed the gene expressions of NF-κB (p65 & p50) and IκBα in inflamed rats paw tissues. In addition, Gelam honey inhibited the nuclear translocation and activation of NF-κB and decreased the cytosolic degradation of IκBα dose dependently in inflamed rats paw tissues. The immunohistochemical expressions of pro-inflammatory mediators COX-2 and TNF-α were also decreased in inflamed rats paw tissues when treated with Gelam honey. The results of our findings suggest that Gelam honey exhibits its inhibitory effects by attenuating NF-κB translocation to the nucleus and inhibiting IκBα degradation, with subsequent decrease of inflammatory mediators COX-2 and TNF-α.

Show MeSH
Related in: MedlinePlus