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An application of outer membrane protein p6-specific enzyme-linked immunosorbent assay for detection of haemophilus influenzae in middle ear fluids and nasopharyngeal secretions.

Hotomi M, Togawa A, Kono M, Sugita G, Sugita R, Fujimaki Y, Kamide Y, Uchizono A, Kanesada K, Sawada S, Okitsu N, Masuda H, Tanaka H, Tanaka Y, Yamanaka N - PLoS ONE (2013)

Bottom Line: An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients.However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly.Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.

ABSTRACT
An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.

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Related in: MedlinePlus

Distribution of the number of copies of the H.influenzae ompP1 gene in MEFs and NPSs based on the results of P6-ELISA and conventional culture.Vertical axis: the number of copies of the ompP1 gene calculated from real-time PCR. Open circle: H. influenzae culture negative, closed circle: H. influenzae culture positive. The number of copies of the H. influenzae ompP1 gene in ODK-0902-positive arid culture-positive populations differed significantly between ODK-0902-positive and -negative populations (p<0.001).
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pone-0071774-g001: Distribution of the number of copies of the H.influenzae ompP1 gene in MEFs and NPSs based on the results of P6-ELISA and conventional culture.Vertical axis: the number of copies of the ompP1 gene calculated from real-time PCR. Open circle: H. influenzae culture negative, closed circle: H. influenzae culture positive. The number of copies of the H. influenzae ompP1 gene in ODK-0902-positive arid culture-positive populations differed significantly between ODK-0902-positive and -negative populations (p<0.001).


An application of outer membrane protein p6-specific enzyme-linked immunosorbent assay for detection of haemophilus influenzae in middle ear fluids and nasopharyngeal secretions.

Hotomi M, Togawa A, Kono M, Sugita G, Sugita R, Fujimaki Y, Kamide Y, Uchizono A, Kanesada K, Sawada S, Okitsu N, Masuda H, Tanaka H, Tanaka Y, Yamanaka N - PLoS ONE (2013)

Distribution of the number of copies of the H.influenzae ompP1 gene in MEFs and NPSs based on the results of P6-ELISA and conventional culture.Vertical axis: the number of copies of the ompP1 gene calculated from real-time PCR. Open circle: H. influenzae culture negative, closed circle: H. influenzae culture positive. The number of copies of the H. influenzae ompP1 gene in ODK-0902-positive arid culture-positive populations differed significantly between ODK-0902-positive and -negative populations (p<0.001).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3756020&req=5

pone-0071774-g001: Distribution of the number of copies of the H.influenzae ompP1 gene in MEFs and NPSs based on the results of P6-ELISA and conventional culture.Vertical axis: the number of copies of the ompP1 gene calculated from real-time PCR. Open circle: H. influenzae culture negative, closed circle: H. influenzae culture positive. The number of copies of the H. influenzae ompP1 gene in ODK-0902-positive arid culture-positive populations differed significantly between ODK-0902-positive and -negative populations (p<0.001).
Bottom Line: An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients.However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly.Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.

ABSTRACT
An enzyme-linked immunosorbent assay specific to outer membrane protein P6 (P6-ELISA) was applied for detecting Haemophilus influenzae in middle ear fluids (MEFs) from acute otitis media (AOM) patients and in nasopharyngeal secretions (NPSs) from acute rhinosinusitis patients. P6-ELISA had a sensitivity of 83.3% for MEFs and 71.5% for NPSs and a specificity of 85.6% for MEFs and 92.5% for NPSs, respectively. Real-time PCR exhibited significant differences in the number of ompP1 gene copies among samples determined by P6-ELISA to be positive and negative for H. influenzae. However, because the P6-ELISA test has the reactivity in Haemophilus species include two commensals H. haemolyticus and H. parainfluenzae, it is thus a weak method in order to detect only NTHi correctly. Consequently, diagnosis using the P6-ELISA should be based on an overall evaluation, including the results of other related examinations and clinical symptoms to prevent misleading conclusions in clinical setting.

Show MeSH
Related in: MedlinePlus