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Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

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Related in: MedlinePlus

Dynamic expression of Notch pathway components in Leydig cells during mouse post-natal (pn) testis development.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Expression of Dll1, Dll4, Notch2 and Notch3 is observed at pn day 4 (4 d) (A–D), while expression of Dll1, Notch1, Notch2 and Notch3 is present at pn day 15 (15 d) (G–J). Notch1, Notch2 and Notch3 continue to be expressed at 1 month pn (1 M) (M–O). Leydig cells were co-localized in twin slides with the anti-3β-HSD antibody (E,K,P). Control was done with rabbit IgG (F,L,Q).
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pone-0072767-g009: Dynamic expression of Notch pathway components in Leydig cells during mouse post-natal (pn) testis development.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Expression of Dll1, Dll4, Notch2 and Notch3 is observed at pn day 4 (4 d) (A–D), while expression of Dll1, Notch1, Notch2 and Notch3 is present at pn day 15 (15 d) (G–J). Notch1, Notch2 and Notch3 continue to be expressed at 1 month pn (1 M) (M–O). Leydig cells were co-localized in twin slides with the anti-3β-HSD antibody (E,K,P). Control was done with rabbit IgG (F,L,Q).

Mentions: Leydig cells were identified in the interstitial space through the presence of 3β-HSD, an enzyme of the steroidogenic metabolic pathway (Figure 9 E,K,P). At pn day 4, Dll1, Dll4, Notch2 and Notch3 are expressed in these cells (Figure 9 A–D), whereas at pn day 15, expression of Dll1, Notch1, Notch2 and Notch3 is identified (Figure 9 G–J). From 1 month of life onwards only the Notch receptors continue to be expressed in Leydig cells (Figure 9 M–O). However, in adulthood, endothelial cells of blood vessels present in the interstitial space express Jagged1 (Figure 10).


Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Dynamic expression of Notch pathway components in Leydig cells during mouse post-natal (pn) testis development.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Expression of Dll1, Dll4, Notch2 and Notch3 is observed at pn day 4 (4 d) (A–D), while expression of Dll1, Notch1, Notch2 and Notch3 is present at pn day 15 (15 d) (G–J). Notch1, Notch2 and Notch3 continue to be expressed at 1 month pn (1 M) (M–O). Leydig cells were co-localized in twin slides with the anti-3β-HSD antibody (E,K,P). Control was done with rabbit IgG (F,L,Q).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755970&req=5

pone-0072767-g009: Dynamic expression of Notch pathway components in Leydig cells during mouse post-natal (pn) testis development.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Expression of Dll1, Dll4, Notch2 and Notch3 is observed at pn day 4 (4 d) (A–D), while expression of Dll1, Notch1, Notch2 and Notch3 is present at pn day 15 (15 d) (G–J). Notch1, Notch2 and Notch3 continue to be expressed at 1 month pn (1 M) (M–O). Leydig cells were co-localized in twin slides with the anti-3β-HSD antibody (E,K,P). Control was done with rabbit IgG (F,L,Q).
Mentions: Leydig cells were identified in the interstitial space through the presence of 3β-HSD, an enzyme of the steroidogenic metabolic pathway (Figure 9 E,K,P). At pn day 4, Dll1, Dll4, Notch2 and Notch3 are expressed in these cells (Figure 9 A–D), whereas at pn day 15, expression of Dll1, Notch1, Notch2 and Notch3 is identified (Figure 9 G–J). From 1 month of life onwards only the Notch receptors continue to be expressed in Leydig cells (Figure 9 M–O). However, in adulthood, endothelial cells of blood vessels present in the interstitial space express Jagged1 (Figure 10).

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

Show MeSH
Related in: MedlinePlus