Limits...
Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

Show MeSH

Related in: MedlinePlus

Jagged1 translocation within the residual bodies.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Jagged1 is located in the elongated spermatids’ cytoplasm at stage V (A). At stage VIII, Jagged1 is located in the residual bodies at the sperm head tip (B). Following release of sperm cells from the seminiferous epithelium, at stage IX, residual bodies containing Jagged1 are progressively translocated towards the basement membrane and localized near the Sertoli cells nuclei (C–D). Bold arrow point to residual bodies containing Jagged1 in the sperm head tip. Arrow heads point to residual bodies containing Jagged1 at the luminal surface of the seminiferous epithelium. Arrows point to residual bodies containing Jagged1 near Sertoli cells nuclei. Asterisks mark Sertoli cells.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3755970&req=5

pone-0072767-g007: Jagged1 translocation within the residual bodies.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Jagged1 is located in the elongated spermatids’ cytoplasm at stage V (A). At stage VIII, Jagged1 is located in the residual bodies at the sperm head tip (B). Following release of sperm cells from the seminiferous epithelium, at stage IX, residual bodies containing Jagged1 are progressively translocated towards the basement membrane and localized near the Sertoli cells nuclei (C–D). Bold arrow point to residual bodies containing Jagged1 in the sperm head tip. Arrow heads point to residual bodies containing Jagged1 at the luminal surface of the seminiferous epithelium. Arrows point to residual bodies containing Jagged1 near Sertoli cells nuclei. Asterisks mark Sertoli cells.

Mentions: Dll1 starts to be expressed in the nuclear part of elongated spermatids when these cells begin to differentiate, at stage IX–X, and is expressed until stage III–IV (Figure 4 E, F, A and B). Notch2, Notch3 and Dll4 are also expressed in elongated spermatids at all stages but only in the cytoplasm (Figure 3 G–L, M–R and Figure 4 G–L, respectively). Jagged1 is located in the residual bodies of elongated spermatids between stage XI–XII and stage VII–VIII (Figure 4 R and M–P). This ligand seems to be translocated from the lumen to the basal region of the seminiferous tubules (Figure 7 A–D). This pattern is associated, following the release of sperm cells within the lumen of seminiferous tubules, with the engulfment of spermatids’ residual bodies by Sertoli cells and their transfer to the basement membrane region of the seminiferous tubule (Figure 7 C–D).


Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Jagged1 translocation within the residual bodies.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Jagged1 is located in the elongated spermatids’ cytoplasm at stage V (A). At stage VIII, Jagged1 is located in the residual bodies at the sperm head tip (B). Following release of sperm cells from the seminiferous epithelium, at stage IX, residual bodies containing Jagged1 are progressively translocated towards the basement membrane and localized near the Sertoli cells nuclei (C–D). Bold arrow point to residual bodies containing Jagged1 in the sperm head tip. Arrow heads point to residual bodies containing Jagged1 at the luminal surface of the seminiferous epithelium. Arrows point to residual bodies containing Jagged1 near Sertoli cells nuclei. Asterisks mark Sertoli cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755970&req=5

pone-0072767-g007: Jagged1 translocation within the residual bodies.Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Jagged1 is located in the elongated spermatids’ cytoplasm at stage V (A). At stage VIII, Jagged1 is located in the residual bodies at the sperm head tip (B). Following release of sperm cells from the seminiferous epithelium, at stage IX, residual bodies containing Jagged1 are progressively translocated towards the basement membrane and localized near the Sertoli cells nuclei (C–D). Bold arrow point to residual bodies containing Jagged1 in the sperm head tip. Arrow heads point to residual bodies containing Jagged1 at the luminal surface of the seminiferous epithelium. Arrows point to residual bodies containing Jagged1 near Sertoli cells nuclei. Asterisks mark Sertoli cells.
Mentions: Dll1 starts to be expressed in the nuclear part of elongated spermatids when these cells begin to differentiate, at stage IX–X, and is expressed until stage III–IV (Figure 4 E, F, A and B). Notch2, Notch3 and Dll4 are also expressed in elongated spermatids at all stages but only in the cytoplasm (Figure 3 G–L, M–R and Figure 4 G–L, respectively). Jagged1 is located in the residual bodies of elongated spermatids between stage XI–XII and stage VII–VIII (Figure 4 R and M–P). This ligand seems to be translocated from the lumen to the basal region of the seminiferous tubules (Figure 7 A–D). This pattern is associated, following the release of sperm cells within the lumen of seminiferous tubules, with the engulfment of spermatids’ residual bodies by Sertoli cells and their transfer to the basement membrane region of the seminiferous tubule (Figure 7 C–D).

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

Show MeSH
Related in: MedlinePlus