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Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

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Related in: MedlinePlus

Schematic illustration of expression patterns of Notch pathway components along the spermatogenic cycle.Adapted draw-scheme [28] representing the mouse stages (I–XII) of the cycle of the seminiferous epithelium in the mouse. Cellular associations of layers of the seminiferous tubules, from the basement membrane to the lumen, are drawn with Sertoli cells separating each stage. Spermatogonia (A, In, B); spermatocytes (Pl- preleptotene, L- leptotene, Z- zygotene, P- pachytene, D- diakinesis, Mi- meiotic division); round spermatids (1–8); elongated spermatids (9–16). Localization of expression of Notch pathway components (top – receptors; bottom – ligands) is drawn in different colors, according to legend.
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pone-0072767-g005: Schematic illustration of expression patterns of Notch pathway components along the spermatogenic cycle.Adapted draw-scheme [28] representing the mouse stages (I–XII) of the cycle of the seminiferous epithelium in the mouse. Cellular associations of layers of the seminiferous tubules, from the basement membrane to the lumen, are drawn with Sertoli cells separating each stage. Spermatogonia (A, In, B); spermatocytes (Pl- preleptotene, L- leptotene, Z- zygotene, P- pachytene, D- diakinesis, Mi- meiotic division); round spermatids (1–8); elongated spermatids (9–16). Localization of expression of Notch pathway components (top – receptors; bottom – ligands) is drawn in different colors, according to legend.

Mentions: These expression patterns are illustrated in Figures 3 and 4, and resumed schematically in Figure 5. At adulthood (3 months of life), throughout the spermatogenic cycle, spermatogonia express Notch1 and Notch3 (Figure 3 A–C and M–O, respectively). When spermatogonia differentiate into preleptotene spermatocytes, at stage VII–VIII, these latter cells start to express Notch2 (Figure 3 J). Stage IX–X leptotene spermatocytes only express Dll4 (Figure 4 K). However, when leptotene spermatocytes differentiate into zygotene spermatocytes, at stage XI–XII, these latter cells cease to express the above Notch components.


Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.

Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, Lopes-da-Costa L - PLoS ONE (2013)

Schematic illustration of expression patterns of Notch pathway components along the spermatogenic cycle.Adapted draw-scheme [28] representing the mouse stages (I–XII) of the cycle of the seminiferous epithelium in the mouse. Cellular associations of layers of the seminiferous tubules, from the basement membrane to the lumen, are drawn with Sertoli cells separating each stage. Spermatogonia (A, In, B); spermatocytes (Pl- preleptotene, L- leptotene, Z- zygotene, P- pachytene, D- diakinesis, Mi- meiotic division); round spermatids (1–8); elongated spermatids (9–16). Localization of expression of Notch pathway components (top – receptors; bottom – ligands) is drawn in different colors, according to legend.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755970&req=5

pone-0072767-g005: Schematic illustration of expression patterns of Notch pathway components along the spermatogenic cycle.Adapted draw-scheme [28] representing the mouse stages (I–XII) of the cycle of the seminiferous epithelium in the mouse. Cellular associations of layers of the seminiferous tubules, from the basement membrane to the lumen, are drawn with Sertoli cells separating each stage. Spermatogonia (A, In, B); spermatocytes (Pl- preleptotene, L- leptotene, Z- zygotene, P- pachytene, D- diakinesis, Mi- meiotic division); round spermatids (1–8); elongated spermatids (9–16). Localization of expression of Notch pathway components (top – receptors; bottom – ligands) is drawn in different colors, according to legend.
Mentions: These expression patterns are illustrated in Figures 3 and 4, and resumed schematically in Figure 5. At adulthood (3 months of life), throughout the spermatogenic cycle, spermatogonia express Notch1 and Notch3 (Figure 3 A–C and M–O, respectively). When spermatogonia differentiate into preleptotene spermatocytes, at stage VII–VIII, these latter cells start to express Notch2 (Figure 3 J). Stage IX–X leptotene spermatocytes only express Dll4 (Figure 4 K). However, when leptotene spermatocytes differentiate into zygotene spermatocytes, at stage XI–XII, these latter cells cease to express the above Notch components.

Bottom Line: The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle.Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern.This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

View Article: PubMed Central - PubMed

Affiliation: Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.

ABSTRACT
The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.

Show MeSH
Related in: MedlinePlus