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A small peptide with therapeutic potential for inflammatory acne vulgaris.

Zhang Z, Mu L, Tang J, Duan Z, Wang F, Wei L, Rong M, Lai R - PLoS ONE (2013)

Bottom Line: In experimental mice skin colonization model, LZ1 significantly reduced the number of P. acnes colonized on the ear, P. acnes-induced ear swelling, and inflammatory cell infiltration.LZ1 showed little cytotoxicity on human keratinocyte and hemolytic activity on human blood red cells.Furthermore, LZ1 was very stable in human plasma.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China ; University of Chinese Academy of Sciences, Beijing, China.

ABSTRACT
A designed peptide named LZ1 with 15 amino acid residues containing strong antimicrobial activity against bacteria pathogens of acne vulgaris including Propionibacterium acnes, Staphylococcus epidermidis and S. aureus. Especially, it exerted strong anti-P. acnes ability. The minimal inhibitory concentration against three strains of P. acnes was only 0.6 µg/ml, which is 4 times lower than that of clindamycin. In experimental mice skin colonization model, LZ1 significantly reduced the number of P. acnes colonized on the ear, P. acnes-induced ear swelling, and inflammatory cell infiltration. It ameliorated inflammation induced by P. acnes by inhibiting the secretion of inflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β. LZ1 showed little cytotoxicity on human keratinocyte and hemolytic activity on human blood red cells. Furthermore, LZ1 was very stable in human plasma. Combined with its potential bactericidal and anti-inflammatory properties, simple structure and high stability, LZ1 might be an ideal candidate for the treatment of acne.

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Related in: MedlinePlus

Effects of LZ1 and clindamycin epicutaneous application on P. acnes-induced ear swelling.The increase in ear thickness of the left ear was calculated as percentage of the right ear. Data represent mean ± SE of four individual experiments. The values for LZ1- or clindamycin-treated group were significant different from the value for the vehicle group (*P< 0.05, **P< 0.001).
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pone-0072923-g003: Effects of LZ1 and clindamycin epicutaneous application on P. acnes-induced ear swelling.The increase in ear thickness of the left ear was calculated as percentage of the right ear. Data represent mean ± SE of four individual experiments. The values for LZ1- or clindamycin-treated group were significant different from the value for the vehicle group (*P< 0.05, **P< 0.001).

Mentions: Ear swelling induced by P. acnes challenge was observed as illustrated in Figure 3. After one-day P. acnes challenge, the ear thickness of vehicle mice was two times of that of un-challenged mice (the control). The corresponding ear thickness for LZ1 and clindamycin-treated mice was 135% and 147% of that of the control, respectively. No swelling was observed in control mice ear injected only with saline. All mice were recovered gradually. At day 2 to day 5, the ear thickness of vehicle mice was 179%, 150%, 130%, and 110% of that of the control while that of LZ1-treated group was 124%, 120%, 110%, and 102% of that of the control, respectively. For the positive group (clindamycin-treated), the ear thickness was 145%, 130%, 125%, and 105% of that of the control, respectively.


A small peptide with therapeutic potential for inflammatory acne vulgaris.

Zhang Z, Mu L, Tang J, Duan Z, Wang F, Wei L, Rong M, Lai R - PLoS ONE (2013)

Effects of LZ1 and clindamycin epicutaneous application on P. acnes-induced ear swelling.The increase in ear thickness of the left ear was calculated as percentage of the right ear. Data represent mean ± SE of four individual experiments. The values for LZ1- or clindamycin-treated group were significant different from the value for the vehicle group (*P< 0.05, **P< 0.001).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3755965&req=5

pone-0072923-g003: Effects of LZ1 and clindamycin epicutaneous application on P. acnes-induced ear swelling.The increase in ear thickness of the left ear was calculated as percentage of the right ear. Data represent mean ± SE of four individual experiments. The values for LZ1- or clindamycin-treated group were significant different from the value for the vehicle group (*P< 0.05, **P< 0.001).
Mentions: Ear swelling induced by P. acnes challenge was observed as illustrated in Figure 3. After one-day P. acnes challenge, the ear thickness of vehicle mice was two times of that of un-challenged mice (the control). The corresponding ear thickness for LZ1 and clindamycin-treated mice was 135% and 147% of that of the control, respectively. No swelling was observed in control mice ear injected only with saline. All mice were recovered gradually. At day 2 to day 5, the ear thickness of vehicle mice was 179%, 150%, 130%, and 110% of that of the control while that of LZ1-treated group was 124%, 120%, 110%, and 102% of that of the control, respectively. For the positive group (clindamycin-treated), the ear thickness was 145%, 130%, 125%, and 105% of that of the control, respectively.

Bottom Line: In experimental mice skin colonization model, LZ1 significantly reduced the number of P. acnes colonized on the ear, P. acnes-induced ear swelling, and inflammatory cell infiltration.LZ1 showed little cytotoxicity on human keratinocyte and hemolytic activity on human blood red cells.Furthermore, LZ1 was very stable in human plasma.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China ; University of Chinese Academy of Sciences, Beijing, China.

ABSTRACT
A designed peptide named LZ1 with 15 amino acid residues containing strong antimicrobial activity against bacteria pathogens of acne vulgaris including Propionibacterium acnes, Staphylococcus epidermidis and S. aureus. Especially, it exerted strong anti-P. acnes ability. The minimal inhibitory concentration against three strains of P. acnes was only 0.6 µg/ml, which is 4 times lower than that of clindamycin. In experimental mice skin colonization model, LZ1 significantly reduced the number of P. acnes colonized on the ear, P. acnes-induced ear swelling, and inflammatory cell infiltration. It ameliorated inflammation induced by P. acnes by inhibiting the secretion of inflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β. LZ1 showed little cytotoxicity on human keratinocyte and hemolytic activity on human blood red cells. Furthermore, LZ1 was very stable in human plasma. Combined with its potential bactericidal and anti-inflammatory properties, simple structure and high stability, LZ1 might be an ideal candidate for the treatment of acne.

Show MeSH
Related in: MedlinePlus