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Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N - Springerplus (2013)

Bottom Line: Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii.P. mandelii CBS-1 grew optimally at 20°C.When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB.

View Article: PubMed Central - PubMed

Affiliation: Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.

ABSTRACT
A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

No MeSH data available.


Related in: MedlinePlus

1H-NMR spectra of a PHB standard and the PHB produced byP. mandeliiCBS-1.
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Fig4: 1H-NMR spectra of a PHB standard and the PHB produced byP. mandeliiCBS-1.

Mentions: After extracting cells with SDS and isolating PHB as a dried residue as described in Materials and methods, we conducted 1H-NMR analysis (Figure 4). PHB peaks were detected at δ = 5.2, 2.5, and 1.2 ppm, which correspond to a –CH doublet, a –CH2 multiplet, and a –CH3 doublet, respectively (Verlinden et al., 2011; De Rooy et al., 2007). The large peak at δ = 7.3 ppm represents the solvent (CD3Cl), and the peaks at δ = 0.0 ppm and δ = 0.8 ppm represent the internal standard (CH3)4Si. A small peak at δ = 1.6 ppm was attributed to minor contamination of the solvent with H2O. These results were identical to those for the PHB standard. Therefore, we concluded that the only PHA synthesized by strain CBS-1 grown in the presence of sucrose as the carbon source was PHB.Figure 4


Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N - Springerplus (2013)

1H-NMR spectra of a PHB standard and the PHB produced byP. mandeliiCBS-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3755782&req=5

Fig4: 1H-NMR spectra of a PHB standard and the PHB produced byP. mandeliiCBS-1.
Mentions: After extracting cells with SDS and isolating PHB as a dried residue as described in Materials and methods, we conducted 1H-NMR analysis (Figure 4). PHB peaks were detected at δ = 5.2, 2.5, and 1.2 ppm, which correspond to a –CH doublet, a –CH2 multiplet, and a –CH3 doublet, respectively (Verlinden et al., 2011; De Rooy et al., 2007). The large peak at δ = 7.3 ppm represents the solvent (CD3Cl), and the peaks at δ = 0.0 ppm and δ = 0.8 ppm represent the internal standard (CH3)4Si. A small peak at δ = 1.6 ppm was attributed to minor contamination of the solvent with H2O. These results were identical to those for the PHB standard. Therefore, we concluded that the only PHA synthesized by strain CBS-1 grown in the presence of sucrose as the carbon source was PHB.Figure 4

Bottom Line: Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii.P. mandelii CBS-1 grew optimally at 20°C.When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB.

View Article: PubMed Central - PubMed

Affiliation: Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.

ABSTRACT
A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

No MeSH data available.


Related in: MedlinePlus